Effects of storage duration and storage temperature on viability of stored ova of kutum (Rutilus frisii kutum) in ovarian fluid

Effects of post-stripping oocyte ageing and temperature on egg viability rates were studied in kutum (Rutilus frisii kutum). Eggs were retained outside the ovarian cavity (in vitro storage) with ovarian fluid at various temperatures of 4, 10, 12 and 26°C. Stored ova of six female kutum were fertilized separately after 0 (control eggs fertilized prior to storage), 2, 4, 6, and 8 h post-stripping (HPS) using fresh and pooled sperm obtained from five males. Eyeing, hatching and eyed egg mortality rates were recorded as an index of egg viability. The results indicated that the maximum eyeing (87%) and hatching (75%) rates of eggs took place at 0 HPS followed by 8 HPS (> 80 and > 70%, respectively) at 4°C. As storage temperature increased, egg viability decreased to 80, 70 and 50% viable at 8 HPS at 4, 10 and 12°C, respectively. The eggs stored at 26°C lost their viability almost completely after 4 HPS. Eyed-egg mortality increased from 13% at 0 HPS to 48.2% and at 4 HPS, at 26°C. This study demonstrated that the in vitro storage method is an applicable effective fertilization technique that can be used for restocking programs of kutum within 8 h at temperatures ranging from 4 to 12°C.Key words: Rutilus frisii kutum, egg storage, temperature, ovarian fluid

Study of few-body nuclei by Feynman’s continual integrals and hyperspherical functions

Feynman’s continual integrals method for solving N-body ground state problem was implemented using parallel computing. The correctness of calculations for 3-body systems 6Li, 12C was checked by comparison with the results of the expansion into hyperspherical functions. New effective method for the solution of the system of hyperradial equations with cubic splines approximation is used. Feynman’s continual integrals method was also used to study the 7Li nucleus as a 4-body system

Biochemical and histological studies of over-ripened oocyte in the Caspian brown trout (Salmo trutta caspius) to determine biomarkers for egg quality

The aims of the present study were to determine the best time for egg stripping after ovulation and to study oocyte over-ripening in the Caspian brown trout (Salmo trutta caspius). Eggs were retained in the female abdominal cavity for 40 days post ovulation (DPO). Partial volumes of eggs stripped from 10 individually identified females at 10 days intervals and fertilized with a pool of semen obtained from 8 males. The biochemistry and histology of the eggs and the biochemistry of the ovarian fluid were studied. The eyeing and hatching rate of the eggs declined with over-ripening time, which decreased from 90.60±6.28% for eyeing and 86.33±6.82% for hatching in newly ovulated eggs (0–10 DPO) to 1.34±0.67% for eyeing and 0.98±0.49% for hatching in over-ripened eggs (30–40 DPO). However, larval abnormalities remained constant for 30 days after ovulation. During the course of oocyte over-ripening, the pH of the ovarian fluid significantly decreased and the concentration of glucose, protein, calcium, iron, and aspartate aminotransferase activity significantly increased. Moreover, the concentration of protein, triglycerides, and aspartate aminotransferase activity in the eggs changed with over-ripening. In the newly ovulated eggs, the yolk consisted of homogenous tissue and its perivitelline space diameter had no considerable differences. With over-ripening, the yolk became heterogeneous, and while chorion diameter did not change, the perivitelline space diameter varied among different areas. The present study demonstrated that the best time to take Caspian brown trout eggs after ovulation at 7±0.6°C was up to 10 DPO. Among the studied parameters of the egg and ovarian fluid, egg quality was related to both ovarian fluid parameters (pH, protein, aspartate aminotransferase, glucose, cholesterol, triglycerides, calcium) and egg parameters (iron, aspartate aminotransferase), suggesting that these parameters can be used as egg quality biomarkers for Caspian brown trout

The experience and prospective use of alternative energy sources for the development of the remote human settlements of the Murmansk region

The experience and prospects for the use of alternative energy sources for the development of remote settlements in the Murmansk region have been considered in the pape