Preliminary Analysis of the Anti-biofilm Efficacy of Manuka Honey on Extended Spectrum Β-lactamase Producing Escherichia Coli Tem-3 and Klebsiella Pneumoniae Shv18, Associated with Urinary Tract Infections

open access articleUrinary Tract Infections (UTIs) are one of the most common infections in the UK and many other parts of the world. The prevalence of the Extended Spectrum β-Lactamases (ESBLs) producing UTIs, combined with their ability to form a bio film, has significantly risen and is limiting therapeutic options. This study investigated the anti-bio film activity of Manuka honey on two ESBL producing pathogens, Escherichia coli TEM-3 and Klebsiella pneumonia SHV18, commonly found in UTIs. The ESBL production was confirmed by the double disk synergy method used to confirm the ESBL production. The antibacterial activity of Manuka honey was determined using the agar well diffusion method. The Minimum Inhibitory Concentration (MIC) was established using serially diluted honey ranging from 50% to 1.56%. The effect of Manuka honey on the pathogen bio films was analysed using the Tissue Culture Plate method, with an established MIC and under 24h incubation with the honey. The results indicated that K. pneumonia SHV18 is a stronger bio film producer than E. coli TEM 3. 50% (w/v) MIC Manuka honey appears to fully prevent the plank tonic growth of both strains. A significant reduction of 81% of the E. coli TEM3 (p < 0.001) and 52% of the K. pneumonia SHV18 (p = 0.001) bio film biomass was observed. The E. coli bio films were found to be more sensitive to the 50% (w/v) honey dilution than those produced by K. pneumonia. The study indicated the anti-bio film potency of Manuka honey and its potential to become an alternative treatment for the ESBL producing pathogens associated with UTIs

On the Feasibility of Fine-Grained TLS Security Configurations in Web Browsers Based on the Requested Domain Name

Most modern web browsers today sacrifice optimal TLS security for backward compatibility. They apply coarse-grained TLS configurations that support (by default) legacy versions of the protocol that have known design weaknesses, and weak ciphersuites that provide fewer security guarantees (e.g. non Forward Secrecy), and silently fall back to them if the server selects to. This introduces various risks including downgrade attacks such as the POODLE attack [15] that exploits the browsers silent fallback mechanism to downgrade the protocol version in order to exploit the legacy version flaws. To achieve a better balance between security and backward compatibility, we propose a mechanism for fine-grained TLS configurations in web browsers based on the sensitivity of the domain name in the HTTPS request using a whitelisting technique. That is, the browser enforces optimal TLS configurations for connections going to sensitive domains while enforcing default configurations for the rest of the connections. We demonstrate the feasibility of our proposal by implementing a proof-of-concept as a Firefox browser extension. We envision this mechanism as a built-in security feature in web browsers, e.g. a button similar to the \quotes{Bookmark} button in Firefox browsers and as a standardised HTTP header, to augment browsers security

The Distribution of ESBL-Producing Enterobacteriaceae: Leicestershire UK Compared to Worldwide

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Pre competition Anxiety level among junior Rugby football Team Players: Sri Lankan Perspective

This paper examines that the action taken to reduce the pre competition anxiety level among   selected junior male Rugby football team in the Sri Lankan context. The major purpose of  this study  is  to  find  the answer for  the  question,  how  to  reduce  the  pre  competition  anxiety  level  among  selected rugby players. The research was based on  the qualitative method. One group pretest, posttest design of the pre experimental design has been selected in this research. It has got only the treatment group but not the control group. The  sample was  taken  from population  at  the  under  19  years  male  Rugby  football  school  teams  of  western province  in Stratified  sampling  technique. Twenty three male rugby football players were the sample size for this research. The specific training schedule was utilized by researcher  to  reduce  the pre competition  anxiety  level  in  selected  sample within  twelve weeks  of  time  period. Interviews, questionnaire, observations, field notes, tests, photographs, audio and videotaping were used to gather data.  Interviews were used  to  identify  the  research  topic;  questionnaires were  used  to  analyze  the  anxiety level. Charts, graphs, line charts, tables, mean value and Minitab software were used to analyze the data. The researcher found that the implemented action plan helps to answer the research questions. This type of research can be utilized in the future in same context. Key Words: Anxiety level, Pre competition and Rugby foot ball Team Player

Vertical temperature structure of 250 m. water layer around Sri Lanka at the tail-end of the north-east monsoon

Data collected during cruises of the Hoyo Maru in Jan-Mar 1975 are analysed. Data is tabulated to show vertical temp profile, surface water temp and temp gradient. Each of these features is discussed. Thermoclines are shown to be established off the coast of India, their depth varying according to time of year. Upwelling off the Cochin coast is discussed. This occurs during Oct-Nov. Surface temp is considerably influenced by the north-east monsoon. The 'clockwise current' (or 'transparent current) characterised by high salinity, high transparency, and rich nutrient conchs, and which prevails in Jan-May brings oceanic water into the Bay of Bengal and sweeps along the Ceylon coast