Characterization of pyridine nucleotide binding site of UDP-glucose 4-epimerase from Saccharomyces fragilis

UDP-glucose 4-epimerase from Saccharomyces fragilis has 1 mol of NAD firmly bound per mol of the dimeric apoenzyme. This prevents a direct study of the coenzyme binding site of the protein. Dissociation of the dimer with p-chloromercuribenzoate and its reconstitution with exogenous NAD or one of its analogues and 2-mercaptoethanol provides an indirect method of study of the site. Depending on the reconstitution properties, the analogues can be classified in the following groups: (i) analogues that have no affinity for the site; (ii) analogues that have affinity but are not incorporated into the apoenzyme; (iii) analogues that produce catalytically inactive holoenzymes; and (iv) analogues that produce catalytically active holoenzymes. Minimum structural requirements that lead to affinity for the coenzyme site and to binding to the apoenzyme can also be discerned from these studies. Reconstitution with etheno-NAD, a fluorescent analogue of NAD, indicates the presence of a hydrophobic pocket for the adenosine subsite

Study on Noncommutative Representations of Galilean Generators

The representations of Galilean generators are constructed on a space where both position and momentum coordinates are noncommutating operators. A dynamical model invariant under noncommutative phase space transformations is constructed. The Dirac brackets of this model reproduce the original noncommutative algebra. Also, the generators in terms of noncommutative phase space variables are abstracted from this model in a consistent manner. Finally, the role of Jacobi identities is emphasised to produce the noncommuting structure that occurs when an electron is subjected to a constant magnetic field and Berry curvature.Comment: Title changed, new references added, published in Int. J. Mod. Phys.

Many-body localized to ergodic transitions in a system with correlated disorder

We study the transition from a many-body localized phase to an ergodic phase in spin chain with correlated random magnetic fields. Using multiple statistical measures like gap statistics and extremal entanglement spectrum distributions, we find the phase diagram in the disorder-correlation plane, where the transition happens at progressively larger values of the correlation with increasing values of disorder. We then show that one can use the average of sample variance of magnetic fields as a single parameter which encodes the effects of the correlated disorder. The distributions and averages of various statistics collapse into a single curve as a function of this parameter. This also allows us to analytically calculate the phase diagram in the disorder-correlation plane