Length and position of base in the ITS-5.8S-ITS2-28S sequence of the selected isolates (NGES1- NGES10).

<p>Length and position of base in the ITS-5.8S-ITS2-28S sequence of the selected isolates (NGES1- NGES10).</p

Amplicon bands revealed by genus and species specific primers against <i>Naegleria</i> isolates for representative samples.

<p>Genus <i>Naegleria</i> specific amplification (Lane 1–13), Lane 1 Ladder 100bp, Lane 2 Negative control, Lane 3 Positive control, Lane 4–13 Positive samples. <i>N</i>. <i>fowleri</i> specific amplification (14–17), Lane 14. Ladder 100bp, Lane 15 Negative control, Lane 16 Positive control, Lane 17 Positive sample.</p

Multiple sequence alignment for amplicons NGES1-NGES10 using ClustalW program.

<p>Black colour dash (—-) indicates gap, red colour fonts base substitution or base insertion, forward and reverse primers are underlined.</p

Morphological observation of <i>Naegleria</i> -like cyst at 100X magnification.

<p>Zone of <i>Escherichia coli</i> clearing progressed and the number of cysts increased markedly.</p

Overview of geographic sites sampled for <i>Naegleria</i> spp.

<p>^<b>#</b><b>Sample was positive for <i>N</i>. <i>fowleri</i></b></p><p>Overview of geographic sites sampled for <i>Naegleria</i> spp.</p

Result of DNA sequencing of the selected amplicons (NGES1-NGES10).

<p>Result of DNA sequencing of the selected amplicons (NGES1-NGES10).</p

Neighbour-joining tree depicting the relationships between test isolates with amplicons (NGES1-NGES10) and reference strains of <i>Naegleria</i>.

<p>Numbers at the notes are percentage-bootstrapping values on 1000 replicates, and only values of > 50% are shown. GenBank accession numbers for reference sequences are indicated at the ends of the species designations.</p

Graph depicting the association between temperature of the sampling sites and isolation frequency of <i>Naegleria</i> spp.

<p>Y axis denotes the number of isolation and X axis denotes minimum and maximum temperature range of the sampling sites during the entire season (March to August).</p

Surveillance for Influenza-like-Illness in and around Delhi, North India, 2007–2010.

<p>*Denominator for the percentage is # influenza positive for that year.</p

Phylogenetic analysis of HA gene of Influenza A(H1N1)pdm09 strains isolated from Urban (blue) and peri-urban (green) sites from Delhi region, North India in Aug 2009 or 2010.

<p>The representative strains from 2009/A/California(H1N1) strain and from other parts of the world and India (Orange) were used to generate the phylogenetic tree. The characteristic amino acids unique to clade 7 (S203T) is shown at the root of the branch.</p