Linear morphometric variables used in the analysis of morphological differentiation of <i>Calotriton arnoldi</i>.

<p>Linear morphometric variables used in the analysis of morphological differentiation of <i>Calotriton arnoldi</i>.</p

Map showing the geographical distribution of Cyt <i>b</i> DNA haplotypes.

<p>(A) Pie diagram size indicates number of individuals. (B) Statistical parsimony networks showing cytochrome <i>b</i> haplotypes found for <i>Calotriton arnoldi</i> (left) and <i>C. asper</i> (right); circle size are proportional to haplotype abundance, straight lines and black dots reflect mutations and unsampled or extinct haplotype.</p

Discriminant canonical analysis scatterplot of males and females of <i>Calotriton arnoldi</i> from eastern and western sectors.

<p>Red squares and circles indicate eastern males and females, and blue squares and circles show western males and females, respectively. Black points are the mean values for each group.</p

Analysis of molecular variance (AMOVA) between <i>Calotriton asper</i> and <i>C. arnoldi</i>.

*<p>P<0.05, ** P<0.0001.</p

Weight coefficients of eight linear morphometric measurements for the two first canonic from discriminant analysis.

<p>Eigenvalues and percentage of accumulated variance are listed.</p

Predicted distribution models of <i>Calotriton asper</i>.

<p>(A) Present distribution using climate and topographic variables, landcover and lithology. Last Glacial Maximum based on (B) CCSM model and (C) MIROC model, and (D) Last Interglacial distributions using climate variables and lithology. Warmer colors represent areas of high habitat suitability. Red marks and arrows indicate the Montseny Mountain Natural Park.</p

Map showing the geographical distribution of RAG-1 DNA haplotypes.

<p>(A) Pie diagram size indicates number of individuals. (B) Statistical parsimony network showing nuclear haplotypes found for <i>Calotriton</i> genus; circle sizes are proportional to haplotype abundance, straight lines and black dots reflect mutations and unsampled or extinct haplotype.</p

Mitochondrial (Cyt <i>b</i>) and nuclear (RAG-1) DNA diversity statistics for <i>Calotriton asper</i> and <i>C. arnoldi</i>.

<p>Number of sequences (n), haplotypes (<i>h</i>) and variable sites (S), and nuclear (Π) and haplotype diversities (Hd). Standard deviations in brackets.</p

Spatial analysis of molecular variance (SAMOVA) and analysis of molecular variance (AMOVA) for <i>Calotriton arnoldi</i>.

*<p>P<0.05, ** P<0.0001</p><p>Two geographical groupings are showed corresponding to eastern and western sectors.</p

Characteristic patterns of coloration of newts belonging to the two sectors of <i>Calotriton arnoldi</i> range.

<p>(A) Adult female from the eastern populations showing yellow spots found in 78.8% of the individuals, and (B) adult male from the western populations with the whitish margin of the snout.</p