Growth kinetics of <i>B</i>. <i>multivorans</i> ATCC 17616 in the presence of triclosan (1.0 μg/ml) and the indicated outer membrane permeabilizer.

Each value represents the mean of at least three independent determinations. Symbols: (●) control, (▼) triclosan, (■) permeabilizer, (♦) triclosan plus permeabilizer. (TIF)</p

Growth kinetics of <i>P</i>. <i>aeruginosa</i> PAO1 in the presence of novobiocin (10.0 μg/mL) and the indicated outer membrane permeabilizer.

Each value represents the mean of at least three independent determinations. Symbols: (●) control, (○) novobiocin, (▼) permeabilizer, (△) novobiocin plus permeabilizer.</p

Potentiation of NPN uptake by outer membrane permeabilization of test organisms.

Each value represents the mean of at least three independent determinations plus standard error. (A) P. aeruginosa PAO1, (B) B. multivorans BAA-247, (C) B. multivorans ATCC 17616, and (D) B. multivorans CGD2. Abbreviations: Pos, P. multocida ATCC 11039 (positive control); Neg, cells lacking permeabilizer (negative control); 48/80, compound 48/80; PMBN, polymyxin B nonapeptide; PMB, polymyxin B; and EDTA, ethylenediaminetetracetic acid. Comparisons represented by * indicate significant differences (P < 0.05) between positive control and treatment groups, while ** indicate significant differences (P < 0.05) between negative control and treatment groups. Statistically significant differences between treatment group means was determined using a one-way ANOVA with Tukey’s post-hoc pairwise comparisons.</p

Susceptibility to outer membrane impermeant antibacterial agents and polymyxin B.

Susceptibility to outer membrane impermeant antibacterial agents and polymyxin B.</p

Growth kinetics of <i>P</i>. <i>aeruginosa</i> PAO1 in the presence of triclosan (2.0 μg/mL) and the indicated outer membrane permeabilizer.

Each value represents the mean of at least three independent determinations. Symbols: (●) control, (○) triclosan, (▼) permeabilizer, (△) triclosan plus permeabilizer.</p

Growth kinetics of <i>B</i>. <i>multivorans</i> CGD2 in the presence of novobiocin (1.0 μg/ml) and the indicated outer membrane permeabilizer.

Each value represents the mean of at least three independent determinations. Symbols: (●) control, (▼) novobiocin, (■) permeabilizer, (♦) novobiocin plus permeabilizer. (TIF)</p

Growth kinetics of <i>B</i>. <i>multivorans</i> ATCC 17616 in the presence of novobiocin (1.0 μg/ml) and the indicated outer membrane permeabilizer.

Each value represents the mean of at least three independent determinations. Symbols: (●) control, (▼) novobiocin, (■) permeabilizer, (♦) novobiocin plus permeabilizer. (TIF)</p

Growth kinetics of <i>B</i>. <i>multivorans</i> CGD2 in the presence of triclosan (1.0 μg/ml) and the indicated outer membrane permeabililzer.

Each value represents the mean of at least three independent determinations. Symbols: (●) control, (▼) triclosan, (■) permeabilizer, (♦) novobiocin plus permeabilizer. (TIF)</p

Growth kinetics of <i>B</i>. <i>multivorans</i> ATCC BAA-247 (type strain) in the presence of triclosan (1.0 μg/mL) and the indicated outer membrane permeabilizer.

Each value represents the mean of at least three independent determinations. Symbols: (●) control, (○) triclosan, (▼) permeabilizer, (△) triclosan plus permeabilizer.</p

Growth kinetics of <i>B</i>. <i>multivorans</i> ATCC BAA-247 (type strain) in the presence of novobiocin (1.0 μg/mL) and the indicated outer membrane permeabilizer.

Each value represents the mean of at least three independent determinations. Symbols: (●) control, (○) novobiocin, (▼) permeabilizer, (△) novobiocin plus permeabilizer.</p