Relationship of metabolic syndrome and its components with -844 G/A and HindIII C/G PAI-1 gene polymorphisms in Mexican children

<p>Abstract</p> <p>Background</p> <p>Several association studies have shown that -844 G/A and <it>HindIII </it>C/G <it>PAI-1 </it>polymorphisms are related with increase of PAI-1 levels, obesity, insulin resistance, glucose intolerance, hypertension and dyslipidemia, which are components of metabolic syndrome. The aim of this study was to analyze the allele and genotype frequencies of these polymorphisms in <it>PAI-1 </it>gene and its association with metabolic syndrome and its components in a sample of Mexican mestizo children.</p> <p>Methods</p> <p>This study included 100 children with an age range between 6-11 years divided in two groups: a) 48 children diagnosed with metabolic syndrome and b) 52 children metabolically healthy without any clinical and biochemical alteration. Metabolic syndrome was defined as the presence of three or more of the following criteria: fasting glucose levels ≥ 100 mg/dL, triglycerides ≥ 150 mg/dL, HDL-cholesterol < 40 mg/dL, obesity BMI ≥ 95^thpercentile, systolic blood pressure (SBP) and diastolic blood pressure (DBP) ≥ 95^thpercentile and insulin resistance HOMA-IR ≥ 2.4. The -844 G/A and <it>HindIII </it>C/G <it>PAI-1 </it>polymorphisms were analyzed by PCR-RFLP.</p> <p>Results</p> <p>For the -844 G/A polymorphism, the G/A genotype (OR = 2.79; 95% CI, 1.11-7.08; <it>p </it>= 0.015) and the A allele (OR = 2.2; 95% CI, 1.10-4.43; <it>p </it>= 0.015) were associated with metabolic syndrome. The -844 G/A and A/A genotypes were associated with increase in plasma triglycerides levels (OR = 2.6; 95% CI, 1.16 to 6.04; <it>p </it>= 0.02), decrease in plasma HDL-cholesterol levels (OR = 2.4; 95% CI, 1.06 to 5.42; <it>p </it>= 0.03) and obesity (OR = 2.6; 95% CI, 1.17-5.92; <it>p </it>= 0.01). The C/G and G/G genotypes of the <it>HindIII </it>C/G polymorphism contributed to a significant increase in plasma total cholesterol levels (179 vs. 165 mg/dL; <it>p </it>= 0.02) in comparison with C/C genotype.</p> <p>Conclusions</p> <p>The -844 G/A <it>PAI-1 </it>polymorphism is related with the risk of developing metabolic syndrome, obesity and atherogenic dyslipidemia, and the <it>HindIII </it>C/G <it>PAI-1 </it>polymorphism was associated with the increase of total cholesterol levels in Mexican children.</p

Presence of indicator bacteria, diarrhoeagenic Escherichia coli pathotypes and Salmonella in fresh carrot juice from Mexican restaurants

We studied the association of age, gender, and distribution of body fat with prehypertension in a sample of Mexican adults. This study was performed in a sample of 900 adults (275 men and 625 women), with the median age of 42 years. Resting blood pressure was measured in duplicate, and prehypertension and hypertension were defined according to JNC 7 criteria. The prevalence of hypertension and prehypertension in our population was 11.56% and 26.5%, respectively. The prevalence of prehypertension was significantly higher in men than in women. Prehypertension was associated with middle and old age (odds ratio [OR] = 2.6 and 2.4, respectively, P < .001), abdominal obesity (OR = 1.3, P = .008), upper quintiles of body mass index (OR = 2.05, P = .005), waist (OR = 1.97, P = .01) and hip (OR = 2.04, P = .005) circumferences, and body fat (OR = 2.37, P = .001). The main factors associated with the development of prehypertension are age, central obesity, and body fat. " 2013 Informa Healthcare USA, Inc.",,,,,,"10.3109/10641963.2012.690470",,,"http://hdl.handle.net/20.500.12104/43813","http://www.scopus.com/inward/record.url?eid=2-s2.0-84871733758&partnerID=40&md5=4647987ceca5c48a7090113d77945259",,,,,,"1",,"Clinical and Experimental Hypertension",,"6

Prehypertension in a Mexican population: Influence of age, gender, and body fat

We studied the association of age, gender, and distribution of body fat with prehypertension in a sample of Mexican adults. This study was performed in a sample of 900 adults (275 men and 625 women), with the median age of 42 years. Resting blood pressure was measured in duplicate, and prehypertension and hypertension were defined according to JNC 7 criteria. The prevalence of hypertension and prehypertension in our population was 11.56% and 26.5%, respectively. The prevalence of prehypertension was significantly higher in men than in women. Prehypertension was associated with middle and old age (odds ratio [OR] = 2.6 and 2.4, respectively, P < .001), abdominal obesity (OR = 1.3, P = .008), upper quintiles of body mass index (OR = 2.05, P = .005), waist (OR = 1.97, P = .01) and hip (OR = 2.04, P = .005) circumferences, and body fat (OR = 2.37, P = .001). The main factors associated with the development of prehypertension are age, central obesity, and body fat. © 2013 Informa Healthcare USA, Inc

CD36 haplotypes are associated with lipid profile in normal-weight subjects

Background: Dyslipidemia is a common metabolic disorder that may result from abnormalities in the synthesis, processing and catabolism of lipoprotein particles. Disorders of lipoprotein concentrations and elevated concentration of oxidized lipoproteins (oxLDL) are risk factors in the pathogenesis of cardiovascular diseases (CVD). CD36 plays an important role in lipid metabolism and polymorphisms in the CD36 gene are related to cardiovascular risk factors. The purpose of this study was to evaluate whether there is an association between genotypes and haplotypes of five polymorphisms in the CD36 gene with lipid levels in young normal-weight subjects. Methods. A total of 232 unrelated subjects with normal-weight of 18 to 25 years old (157 women and 75 men) were randomly selected. The lipid profile and glucose levels were measured by enzymatic colorimetric assays. Genotyping of the polymorphisms -33137A/G (rs1984112), -31118G/A (rs1761667), -22674 T/C (rs2151916), 27645 Ins/Del (rs3840546) and 30294G/C (rs1049673) in the CD36 receptor gene was performed by polymerase chain reaction and restriction fragment length polymorphism, linkage disequilibrium analysis among the five polymorphisms and an analysis of haplotype were estimated. Results: HDL-C levels was lower in men than in women (P = 0.03). However, the median oxLDL levels in men was higher than in women (P = 0.05). There was no significant difference in the levels of TC, TG, LDL-C and glucose (P > 0.05). HDL-C levels were lower in the subjects with TC genotype of polymorphism -22674 T/C (P = 0.04), but the carriers of TT genotype had lower oxLDL levels (P = 0.01). LDL-C levels were higher in young carriers of CC genotype for 30294G/C polymorphism than non-carriers (P = 0.03). The subjects carrying the AATDC haplotype had 3.2 times presumably higher risk of LDL-C > 100 mg/dL than the carrying the AGTIG haplotype (P = 0.02), whereas the subjects carrying the AATIC haplotype had 2.0 times presumably higher risk of TC > 200 mg/dL than the carrying the AGTIC haplotype (P = 0.02). Conclusion: The study provides evidence of a genetic association of CD36 haplotypes with the variability in LDL-C and TC levels in a sample of normal-weight subjects. Zapotitlán 2013 Ramos-Arellano et al.; licensee BioMed Central Ltd

Increase levels of apo-A1 and apo B are associated in knee osteoarthritis: Lack of association with VEGF-460 T/C and +405 C/G polymorphisms

To investigate the genotype and allele frequency of vascular endothelial growth factor gene polymorphisms in knee osteoarthritis (OA) and their relationship with disease activity and lipid profile, we enrolled 49 knee OA patients and 75 healthy subjects (HS) as a control group. Body mass index (BMI), laboratorial assessment and genotyped by polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) were studied in both groups. Disease activity was determined using Lequesne and WOMAC indexes; a P value < 0.05 was considered significant. The -460 and +405 VEGF polymorphisms did not shown significant association between OA patients and HS. However, between OA patients and HS a significant differences were observed in BMI, age, apo A-I and apo B, independently of both polymorphisms studied (P < 0.05). In conclusion, increased apo A-1 and apo B levels are associated in knee OA, but the -460 T/C and +405 C/G VEGF polymorphisms are not associated with knee OA susceptibility. � 2008 Springer-Verlag

Regulation of the organic pollution level in anaerobic digesters by using off-line COD measurements

Background: Several association studies have shown that -844 G/A and HindIII C/G PAI-1 polymorphisms are related with increase of PAI-1 levels, obesity, insulin resistance, glucose intolerance, hypertension and dyslipidemia, which are components of metabolic syndrome. The aim of this study was to analyze the allele and genotype frequencies of these polymorphisms in PAI-1 gene and its association with metabolic syndrome and its components in a sample of Mexican mestizo children.Methods: This study included 100 children with an age range between 6-11 years divided in two groups: a) 48 children diagnosed with metabolic syndrome and b) 52 children metabolically healthy without any clinical and biochemical alteration. Metabolic syndrome was defined as the presence of three or more of the following criteria: fasting glucose levels ? 100 mg/dL, triglycerides ? 150 mg/dL, HDL-cholesterol &lt; 40 mg/dL, obesity BMI ? 95 thpercentile, systolic blood pressure (SBP) and diastolic blood pressure (DBP) ? 95 thpercentile and insulin resistance HOMA-IR ? 2.4. The -844 G/A and HindIII C/G PAI-1 polymorphisms were analyzed by PCR-RFLP.Results: For the -844 G/A polymorphism, the G/A genotype (OR = 2.79; 95% CI, 1.11-7.08; p = 0.015) and the A allele (OR = 2.2; 95% CI, 1.10-4.43; p = 0.015) were associated with metabolic syndrome. The -844 G/A and A/A genotypes were associated with increase in plasma triglycerides levels (OR = 2.6; 95% CI, 1.16 to 6.04; p = 0.02), decrease in plasma HDL-cholesterol levels (OR = 2.4; 95% CI, 1.06 to 5.42; p = 0.03) and obesity (OR = 2.6; 95% CI, 1.17-5.92; p = 0.01). The C/G and G/G genotypes of the HindIII C/G polymorphism contributed to a significant increase in plasma total cholesterol levels (179 vs. 165 mg/dL; p = 0.02) in comparison with C/C genotype.Conclusions: The -844 G/A PAI-1 polymorphism is related with the risk of developing metabolic syndrome, obesity and atherogenic dyslipidemia, and the HindIII C/G PAI-1 polymorphism was associated with the increase of total cholesterol levels in Mexican children. " 2012 De la Cruz-Mosso et al; licensee BioMed Central Ltd.",,,,,,"10.1186/1471-2431-12-41",,,"http://hdl.handle.net/20.500.12104/44169","http://www.scopus.com/inward/record.url?eid=2-s2.0-84858966556&partnerID=40&md5=aaa17193e779c0fa53b5fcc85c4eb66a",,,,,,,,"BMC Pediatrics",,,,"12",,"Scopu