The Evidence of Non n-glycan Linked Mannose in Exochitinase 42kDa, from Trichoderma harzianum BIO10671 Glycosylation

Chitinase 42 kDa produced by Trichoderma harzianum has been proven as a prime compound to be excreted onto the hyphae of the pathogen causing localised cell wall lysis at the point of interaction. Later it will initiate the process of the host cell becomes empty of cytoplasm, disintegrates and shows a rapid collapse. This study investigates the existence of N-glycan linked mannose in chitinase 42 kDa produced by the Malaysian T. harzianum strain BIO10671. The chitinase 42 kDa from T. harzianum BIO10671 was initially purified using anion exchange chromatography prior to a series of experiments such as immunoblotting against the chitinase 42 kDa antibody, lectin staining for detecting any terminal linked mannose, and galactofuranose detection to determine the presence of galatofuranase components in glycoproteins. The enzyme purification harvested about 12-fold of chitinase 42 kDa from T. harzianum BIO10671 with strong indication of the chitinase 42 kDa presence on SDS-Page. This was confirmed by immunoblotting with a strong response around 42 kDa after overnight incubation in chitinase 42 kDa antibody suggesting that the gene for chitinase 42 kDa was greatly expressed in this strain. There are no intervation of galatofuranose on any of the terminal mannose in chitinase 42 kDa as shown by negative results on samples treated with or without endoglycosidase-H and lectin staining. Therefore, it can be concludeed that glycosylation occurred in the chitinase 42 kDa from T. harzianum 42 kDa was not in the form of N-glycan linked mannose as expected

How social media crisis response and social interaction is helping people recover from Covid-19: an empirical investigation

Community resilience following a crisis has become essential to avoid panic. In contrast, social media usage has been practical to improve public resilience. However, the impacts of social media crisis response and social interaction have not been fully addressed. Therefore, this study aims to investigate the effects of social media crisis communication on public resilience. The study data were collected through an online medium, and the final responses consist of 393 observations, mainly of Malaysians who have experienced Covid-19 isolation, quarantine, or lockdown. The assessments of the reflective measurement models based on path analysis in PLS-SEM are reliable and valid. The Cronbach’s alpha, rho_A, composite reliability, and discriminant validity revealed acceptable values. PLS prediction algorithm was run to assess the model’s predictive power, and the findings show that the predictive relevance is satisfactory. Furthermore, the IPMA was applied to evaluate the model’s usefulness, which compares the level of the variables from the performance scale mean value against the importance level. The result shows that all the variables are useful and reveal good performance. Thus, crisis management and communication activities should pay more attention to these variables for effective social media crisis communication. Thus, the study offers theoretical and practical implications in the field of social media-based crisis communication and crisis informatics