3 research outputs found
Effects of Three-Dimensional Culture of Mouse Calvaria-Derived Osteoblastic Cells in a Collagen Gel with a Multichannel Structure on the Morphogenesis Behaviors of Engineered Bone Tissues
Bone
has a complex hierarchical structure that contributes to its
superior mechanical properties. Therefore, reproducing the complex
hierarchical structure of bone tissue is a promising strategy to construct
functional engineered bone tissues. In this study, we aimed to reproduce
this complex hierarchical structure by developing a method for the
three-dimensional culture of MC3T3-E1 osteoblastic cells in a collagen
gel with a multichannel structure (MCCG), which mimics the parallel
arrangement of Haversian canals in bone tissue. MCCG was homogeneously
calcified via the biomineralization properties of MC3T3-E1s. Confocal
laser scanning microscopy revealed that MCCG could support the growth
and proliferation of MC3T3-E1 cells in the deeper parts of the engineered
bone tissue and that the cells formed a toroidal structure on the
channel surface and a network-like structure in the gel matrix region.
Furthermore, quasi-quantitative measurement of osteocalcin and dentin
matrix protein 1 expression indicated the coexistence of two types
of cells with different morphologies and differentiation phenotypes.
Thus, three-dimensional culture of MC3T3-E1 cells in MCCG yielded
engineered tissues mimicking the hierarchical structures of bone tissues.
Engineered bone tissues with a biomimetic hierarchical structure could
be used as a model system for investigating bone metabolism and evaluating
the efficacy of novel drugs
Application of Multichannel Collagen Gels in Construction of Epithelial Lumen-like Engineered Tissues
Introduction
of epithelial lumen-like structures such as blood
and lymphatic vessels, as well as renal tubules, is a prerequisite
for successful construction and function of artificially engineered
giant tissues. Here, we demonstrate a methodology for construction
of various epithelial lumen-like structures by using multichannel
collagen gels (MCCGs). MCCGs were prepared and used as template scaffolds
for constructing epithelial lumen structures in a controlled fashion.
The effect of NaCl concentration on the multichannel structure of
MCCGs was investigated by using confocal laser scanning microscopy
along with fluorescent staining. The channel diameter increased with
increasing NaCl concentrations in the collagen solution and the phosphate
buffer solution. In contrast, the channel number decreased with increasing
NaCl concentrations. Engineered tissues with various lumen-like structures
were constructed by seeding and culturing Madin–Darby canine
kidney cells on MCCGs. The diameter of the lumen and the number of
lumens per unit area were controllable by regulating the multichannel
structure of cylindrical MCCG. We believe that our methodology for
the construction of engineered tissues possessing epithelial lumen-like
structures will prove helpful in regeneration of giant tissues with
various hierarchical structures
Application of Multichannel Collagen Gels in Construction of Epithelial Lumen-like Engineered Tissues
Introduction
of epithelial lumen-like structures such as blood
and lymphatic vessels, as well as renal tubules, is a prerequisite
for successful construction and function of artificially engineered
giant tissues. Here, we demonstrate a methodology for construction
of various epithelial lumen-like structures by using multichannel
collagen gels (MCCGs). MCCGs were prepared and used as template scaffolds
for constructing epithelial lumen structures in a controlled fashion.
The effect of NaCl concentration on the multichannel structure of
MCCGs was investigated by using confocal laser scanning microscopy
along with fluorescent staining. The channel diameter increased with
increasing NaCl concentrations in the collagen solution and the phosphate
buffer solution. In contrast, the channel number decreased with increasing
NaCl concentrations. Engineered tissues with various lumen-like structures
were constructed by seeding and culturing Madin–Darby canine
kidney cells on MCCGs. The diameter of the lumen and the number of
lumens per unit area were controllable by regulating the multichannel
structure of cylindrical MCCG. We believe that our methodology for
the construction of engineered tissues possessing epithelial lumen-like
structures will prove helpful in regeneration of giant tissues with
various hierarchical structures