Soft corrugated channel with synergistic exclusive discrimination gating for CO₂ recognition in gas mixture

二酸化炭素に対してのみゲートを開いて吸着する フレキシブル多孔性材料を開発. 京都大学プレスリリース. 2023-08-02.Interactive networks for capturing gas with high selectivity. 京都大学プレスリリース. 2023-08-02.Developing artificial porous systems with high molecular recognition performance is critical but very challenging to achieve selective uptake of a particular component from a mixture of many similar species, regardless of the size and affinity of these competing species. A porous platform that integrates multiple recognition mechanisms working cooperatively for highly efficient guest identification is desired. Here, we designed a flexible porous coordination polymer (PCP) and realised a corrugated channel system that cooperatively responds to only target gas molecules by taking advantage of its stereochemical shape, location of binding sites, and structural softness. The binding sites and structural deformation act synergistically, exhibiting exclusive discrimination gating (EDG) effect for selective gate-opening adsorption of CO₂ over nine similar gas molecules, including N₂, CH₄, CO, O₂, H₂, Ar, C₂H₆, and even higher-affinity gases such as C₂H₂ and C₂H4. Combining in-situ crystallographic experiments with theoretical studies, it is clear that this unparalleled ability to decipher the CO₂ molecule is achieved through the coordination of framework dynamics, guest diffusion, and interaction energetics. Furthermore, the gas co-adsorption and breakthrough separation performance render the obtained PCP an efficient adsorbent for CO₂ capture from various gas mixtures

Cold Spray Powders and Equipment

Chapter 3A particle shock consolidation encountered in the coldspray (CS) process is defined by a highvelocity impact of powder particles onto the substrate. The particle impact results in the generation of high stresses and strains both in the particles and the substrate. As shown in the basic monographs (Champagne, 2007; Papyrin et al., 2007), the powder material to be sprayed must feature sufficient ductility to ensure particle strains and cold welding without its failure. In some cases (at very high particle velocities) Ti alloy particle melting occurs (Vlcek et al., 2002). However, impact of other metallic powder materials is characterized by high stresses and particle shear strains. Because a lot of information is available on various powders and powder mixtures developed and applied for cold spraying (Jeandin et al., 2014; Moridi et al., 2014, and others), the goal of this chapter is to describe and discuss the concept of CS material selection, basic criteria for evaluation of its suitability for cold spraying, and particle behavior during the deposition process (acceleration and formation of interfaces)Book chapterPaolo Matteazzi , Alberto Colella , Volf Leshchynsky , Kazuhiko Sakaki et al. "Chapter 3. Cold Spray Powders and Equipment". Cold Gas Dynamic Spray. Roman Gr. Maev, Volf Leshchynsky, eds. CRC Press, 2016, p.95-118.boo

Expression of interferon-stimulated gene 20 in vascular endothelial cells.

ISG20 is an ribonuclease specific for single-stranded RNA and considered to play a role in innate immunity against virus infections. We herein show that both poly IC, an authentic double-stranded RNA, and IFN-gamma induced ISG20 expression in cultured HUVEC. Poly IC-induced ISG20 expression was inhibited by LY294002, an inhibitor of PI3K, or by RNA interference against IFN regulatory factor three. ISG20 expression was not induced by IFN-beta, loxoribine or CpG oligonucleotide. These results suggest that ISG20 induction by poly IC may not be dependent on the IRF-3-mediated type I IFN induction pathway in HUVEC. ISG20 may be involved in innate immunity against viral infection in vascular endothelial cells

HLA-DR8 Subtyping by Polymerase Chain Reaction (PCR) - DNA Conformation Polymorphism (DCP) Analysis : a simple and practical genotyping method

Two hundred Japanese panels were serologically typed for human leukocyte antigen (HLA) - DR to assign 65 HLA-DRS haplotypes, which were then subdivided into two genotypes, i.e., DRB1 *0802 and DRB1 *0803, by a polymerase chain reaction (PCR) - based, simple, and practical method. The panels possessing DR8 specificity were firstly subjected to PCR with a couple of primers specifically to amplify their DR52 associated group - DRB1 genes. PCR products were then denatured in the presence of formamide, electrophoresed in a non-denaturing polyacrylamide gel, and visualized by silver staining. The same DRB1 products of these samples were also mixed with the DRB1 *1302, and simultaneously analyzed by the same procedure. Electrophoretic mobilities of the samples were compared with those of the typing standards to genotype their DRS-DRB1 alleles by using the characteristic polymorphism in the single-stranded DNAs and the heteroduplexes. This method, designated PCR - DNA conformation polymorphism (DCP) analysis, allowed for genotyping of the DR8-DRB1 alleles without using sequence - specific oligonucleotide probes (SSOP) or restriction endonucleases. The entire process after PCR was completed within a few hours. The tested panels were also genotyped for DRB1 gene by the PCR-SSOP method for comparison with results obtained by the PCR-DCP method. Satisfactory coincidence was achieved and it represented how accurately the new system genotyped DRB1 *0802 and DRB1 *0803. PCR-DCP analysis was thus shown to be practical and useful for subtyping of serologically defined DR8 specificities.This work was supported in part by grants-in-aid from the Ministry of Education, Culture and Science, Japan, and research grants from the Ministry of Health and Welfare, Japan