Cytotoxicity of the Bacillus thuringiensis Crystal Protein against Mammalian Cells

The crystal proteins produced by Bacillus thuringiensis subsp, israelensis (Bti) and subsp. coreanensis A1519 strain were examined for the cytotoxicity against MOLT-4 and HeLa cells by MTT assay and LDH assay, The A1519 crystal proteins processed by proteinase K exhibited the specific cell-killing activity toward MOLT-4 with little damage to the cell membrane, On the other hand, the Bti crystal proteins processed by proteinase K caused the substantial damage to the cell membrane of both MOLT-4 and HeLa, leading to the cell lysis. The non-digested crystal proteins of both strains exhibited no cytotoxicity, These data suggested that while the Bti crystal proteins caused the colloid-osmotic swelling and cell lysis of MOLT-4 and HeLa, the proteinase K-digested A1519 crystal proteins induced the specific cell death of MOLT-4 through a mechanism other than that of Bti

Polarity reversals of remanent magnetization in a sedimentary core from Northwind Ridge, western Arctic Ocean

We studied the remanent magnetization of a sedimentary core PC2 (6.48m length) drilled during the MR99-K05 cruise of JAMSTEC in the western Arctic Ocean. Discrete specimens and u-channel samples were used in the study. In the discrete specimens, the change of remanent magnetization with depth show many clear polarity reversals in both inclination and declination. Furthermore, most reversals in discrete specimens correlated well with those in the u-channel samples. Core PC2 is characterized by distinct alternations of dark gray and brownish layers. Through comparison of lithostratigraphic cycles with glacial-interglacial cycles (referring to R.L. Phillips and A. Grantz, Geol. Soc. Am. Bull., 109, 1101, 1997), sedimentary cycles of core PC2 are correlated to marine isotope stages up to MIS-8. This indicates that polarity reversals of remanent magnetization in core PC2 are geomagnetic excursions in the Brunhes epoch. Comparison with the previously known geomagnetic excursions shows that the polarity reversals are clear and have long duration in core PC2. This feature may be related to characteristic geomagnetism around the western Arctic Ocean

多変数有理型函数の接続の理論

金沢大学理学部研究課題/領域番号:X00090----154018, 研究期間(年度):1976出典：「多変数有理型函数の接続の理論」研究成果報告書　課題番号X00090----154018（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-X00090----154018/）を加工して作

有理型函数の接続の理論(多変数)

金沢大学理学部研究課題/領域番号:X42440----401056, 研究期間(年度):1967出典：研究課題「有理型函数の接続の理論(多変数)」課題番号X42440----401056（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-X42440----401056/）を加工して作

複素解析とその境界領域の研究

金沢大学理学部研究課題/領域番号:X00050----434005, 研究期間(年度):1979 – 1980出典：「複素解析とその境界領域の研究」研究成果報告書　課題番号X00050----434005（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-X00050----434005/）を加工して作

純不連続群と保型函数の研究

金沢大学理学部研究課題/領域番号:X46095-----84510, 研究期間(年度): 1971出典：研究課題「純不連続群と保型函数の研究」課題番号 X46095-----84510（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-X46095-----84510/）を加工して作

The cytotoxicity of Bacillus thuringiensis subsp. coreanensis A2316 strain against the human leukemic T cell

Bacillus thuringiensis subsp. coreanensis A2316 is a newly isolated strain from Yonakunijima Island in Japan. It produces the proteinaceous inclusion body (crystal) which has no insecticidal and hemolytic activities. When the crystal proteins were digested by proteinase K, they exhibited the strong cytotoxicity against human leukemic T cell, MOLT-4. The proteinase K-digested A2316 crystal proteins have little damage upon the cell membrane of MOLT-4, suggesting that the cell death of MOLT-4 was induced through a mechanism other than the colloid-osmotic swelling and cell lysis as caused by hitherto known B. thuringiensis crystal proteins. The 29-kDa polypeptide proved to be an active component of the proteinase K-digested A2316 crystal proteins. EC(50) of the purified 29-kDa polypeptide was 0.0579 μg/ml. The N-terminal amino acid sequence of the 29-kDa polypeptide was identical with that of p29 produced by B. thuringiensis A1519 strain and shared no significant homology with all the known proteins, suggesting that this polypeptide belong to a new family of B. thuringiensis crystal proteins

Isolation of Novel Adenovirus from Fruit Bat (Pteropus dasymallus yayeyamae)

Isolation of Novel Adenovirus from Fruit Ba