On the Embrittlement and Toughness of High Purity Fe-30Cr-2Mo Alloy

Experiments were conducted to explain unexpected embrittlement phenomena encountered in fabricating a high purity Fe-30Cr-2Mo alloy. By means of a hydrostatic tensile test with a Bridgman-Type specimen it was found that the fracture behaviour of the alloy is highly dependent on stress state. Under conditions of low triaxial stress, the alloy displays excellent ductility. Under conditions of high triaxial stress, however, the alloy shows less strain to fracture and a transition from ductile to cleavage fracture. The toughness of the Fe-30Cr-2Mo alloy can be significantly improved by thermomechanical processing

The Infrared Imaging Spectrograph (IRIS) for TMT: optical design of IRIS imager with "Co-axis double TMA"

IRIS (InfraRed Imaging Spectrograph) is one of the first-generation instruments for the Thirty Meter Telescope (TMT). IRIS is composed of a combination of near-infrared (0.84--2.4 $\mu$m) diffraction limited imager and integral field spectrograph. To achieve near-diffraction limited resolutions in the near-infrared wavelength region, IRIS uses the advanced adaptive optics system NFIRAOS (Narrow Field Infrared Adaptive Optics System) and integrated on-instrument wavefront sensors (OIWFS). However, IRIS itself has challenging specifications. First, the overall system wavefront error should be less than 40 nm in Y, z, J, and H-band and 42 nm in K-band over a 34.0 $\times$ 34.0 arcsecond field of view. Second, the throughput of the imager components should be more than 42 percent. To achieve the extremely low wavefront error and high throughput, all reflective design has been newly proposed. We have adopted a new design policy called "Co-Axis double-TMA", which cancels the asymmetric aberrations generated by "collimator/TMA" and "camera/TMA" efficiently. The latest imager design meets all specifications, and, in particular, the wavefront error is less than 17.3 nm and throughput is more than 50.8 percent. However, to meet the specification of wavefront error and throughput as built performance, the IRIS imager requires both mirrors with low surface irregularity after high-reflection coating in cryogenic and high-level Assembly Integration and Verification (AIV). To deal with these technical challenges, we have done the tolerance analysis and found that total pass rate is almost 99 percent in the case of gauss distribution and more than 90 percent in the case of parabolic distribution using four compensators. We also have made an AIV plan and feasibility check of the optical elements. In this paper, we will present the details of this optical system.Comment: 18 pages, 14 figures, Proceeding 9908-386 of the SPIE Astronomical Telescopes + Instrumentation 201

Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues

Adipose-derived stem cells (ASCs) are an attractive cell source for cell therapy. Despite the increasing number of clinical applications, the methodology for ASC isolation is not optimized for every individual. In this study, we developed an effective material to stabilize explant cultures from small-fragment adipose tissues. Methods: Polypropylene/polyethylene nonwoven sheets were coated with hydroxyapatite (HA) particles. Adipose fragments were then placed on these sheets, and their ability to trap tissue was monitored during explant culture. The yield and properties of the cells were compared to those of cells isolated by conventional collagenase digestion. Results: Hydroxyapatite-coated nonwovens immediately trapped adipose fragments when placed on the sheets. The adhesion was stable even in culture media, leading to cell migration and proliferation from the tissue along with the nonwoven fibers. A higher fiber density further enhanced cell growth. Although cells on nonwoven explants could not be fully collected with cell dissociation enzymes, the cell yield was significantly higher than that of conventional monolayer culture without impacting stem cell properties. Conclusions: Hydroxyapatite-coated nonwovens are useful for the effective primary explant culture of connective tissues without enzymatic cell dissociation

A new approach to axial coupling constants in the QCD sum rule

We derive new QCD sum rules for the axial coupling constants by considering two-point correlation functions of the axial-vector currents in a one nucleon state. The QCD sum rules tell us that the axial coupling constants are expressed by nucleon matrix elements of quark and gluon operators which are related to the sigma terms and the moments of parton distribution functions. The results for the iso-vector axial coupling constants and the 8th component of the SU(3) octet are in good agreement with experiment.Comment: 10 pages, 1 figure include

The Infrared Imaging Spectrograph (IRIS) for TMT: Optical design of IRIS imager with "co-axis double TMA"

IRIS (InfraRed Imaging Spectrograph) is one of the first-generation instruments for the Thirty Meter Telescope (TMT). IRIS is composed of a combination of near-infrared (0.84-2.4 μm) diffraction limited imager and integral field spectrograph. To achieve near-diffraction limited resolutions in the near-infrared wavelength region, IRIS uses the advanced adaptive optics system NFIRAOS (Narrow Field Infrared Adaptive Optics System) and integrated on-instrument wavefront sensors (OIWFS). However, IRIS itself has challenging specifications. First, the overall system wavefront error should be less than 40 nm in Y, z, J, and H-band and 42 nm in K-band over a 34.0 × 34.0 arcsecond field of view. Second, the throughput of the imager components should be more than 42 percent. To achieve the extremely low wavefront error and high throughput, all reflective design has been newly proposed. We have adopted a new design policy called "Co-Axis double-TMA", which cancels the asymmetric aberrations generated by "collimator/TMA" and "camera/TMA" efficiently. The latest imager design meets all specifications, and, in particular, the wavefront error is less than 17.3 nm and throughput is more than 50.8 percent. However, to meet the specification of wavefront error and throughput as built performance, the IRIS imager requires both mirrors with low surface irregularity after high-reflection coating in cryogenic and high-level Assembly Integration and Verification (AIV). To deal with these technical challenges, we have done the tolerance analysis and found that total pass rate is almost 99 percent in the case of gauss distribution and more than 90 percent in the case of parabolic distribution using four compensators. We also have made an AIV plan and feasibility check of the optical elements. In this paper, we will present the details of this optical syste

Runx3 regulates folliculogenesis and steroidogenesis in granulosa cells of immature mice

We previously demonstrated that female Runx3 knockout (Runx3-/-) mice were anovulatory and their uteri were atrophic and that Runx3 mRNA was expressed in granulosa cells. To clarify how Runx3 regulates folliculogenesis and ovulation, we examine the effects of Runx3 knockout on the gene expression of growth factors associated with folliculogenesis and enzymes associated with steroidogenesis. In Runx3-/- mouse ovaries, the numbers of primary and antral follicles were lower than those in wild-type (wt) mice at 3 weeks of age, indicating that the loss of Runx3 affects folliculogenesis. The expression of genes encoding activin and inhibin subunits (Inha, Inhba and Inhbb) was also decreased in ovaries from the Runx3-/- mice compared with that in wt mice. Moreover, the expression of the genes Cyp11a1 and Cyp19a1 encoding steroidogenic enzymes was also decreased. In cultured granulosa cells from 3-week-old mouse ovaries, Cyp19a1 mRNA levels were lower in Runx3-/- mice than those in wt mice. Follicle-stimulating hormone (FSH) treatment increased Cyp19a1 mRNA levels in both wt and Runx3-/- granulosa cells in culture but the mRNA level in Runx3-/- granulosa cells was lower than that in wt ones, indicating that granulosa cells could not fully function in the absence of Runx3. At 3 weeks of age, gonadotropin α subunit, FSHβ subunit and luteinizing hormone (LH) β subunit mRNA levels were decreased in Runx3-/- mice. These findings suggest that Runx3 plays a key role in female reproduction by regulating folliculogenesis and steroidogenesis in granulosa cells

The Infrared Imaging Spectrograph (IRIS) for TMT: Instrument Overview

We present an overview of the design of IRIS, an infrared (0.84 - 2.4 micron) integral field spectrograph and imaging camera for the Thirty Meter Telescope (TMT). With extremely low wavefront error (<30 nm) and on-board wavefront sensors, IRIS will take advantage of the high angular resolution of the narrow field infrared adaptive optics system (NFIRAOS) to dissect the sky at the diffraction limit of the 30-meter aperture. With a primary spectral resolution of 4000 and spatial sampling starting at 4 milliarcseconds, the instrument will create an unparalleled ability to explore high redshift galaxies, the Galactic center, star forming regions and virtually any astrophysical object. This paper summarizes the entire design and basic capabilities. Among the design innovations is the combination of lenslet and slicer integral field units, new 4Kx4k detectors, extremely precise atmospheric dispersion correction, infrared wavefront sensors, and a very large vacuum cryogenic system.Comment: Proceedings of the SPIE, 9147-76 (2014