Capturing regional differences in flood vulnerability improves flood loss estimation

Flood vulnerability is quantified by loss models which are developed using either empirical or synthetic approaches. In reality, processes influencing flood risk are stochastic and loss predictions bear significant uncertainty, especially due to differences in vulnerability across exposed objects and regions. However, many state-of-the-art flood loss models are deterministic, i.e., they do not account for data and model uncertainty. The Bayesian Data-Driven Synthetic (BDDS) model was one of the first approaches that used empirical data to reduce the prediction errors at object-level and enhance the reliability of synthetic flood loss models. However, the BDDS model does not account for regional differences in vulnerability which may result in over-/under-estimation of losses in some regions. In order to overcome this limitation, this study introduces a hierarchical parameterization of the BDDS model which enhances synthetic flood loss model predictions by quantifying regional differences in vulnerability. The hierarchical parameterization makes optimal use of the process information contained in the overall data set for the various regional applications, so that it is particularly suitable for cases in which only a small amount of empirical data is available. The implementation and performance of the hierarchical parametrization is demonstrated with the Multi-Colored Manual (MCM) loss functions and empirical damage dataset from the UK consisting of residential buildings from the regions Appleby, Carlisle, Kendal and Cockermouth that suffered losses during the 2015 flood event. The developed model improves prediction accuracy of flood loss compared to MCM by reducing the absolute error and bias by at least 23 and 90%, respectively. The model reliability in terms of hit rate (i.e., the probability that the observed value lies in the 90% high density interval of predictions) is 88% for residential buildings from the same regions used for calibration and 73% for residential buildings from new regions. The approach is of high practical relevance for all regions where only limited amounts of empirical flood loss data is available

Involvement of luteinizing hormone in the implantation process of the rat

The use of specific anti-FSH and anti-LH substances has shown that LH is the only pituitary gonadotrophin involved in the implantation process. Using different dosages of LH antiserum at different time intervals, it has been possible to arrive at a minimum effective dose (0.05 ml) which, when given on the 4th day at 10.00 hours, results in inhibition of implantation on the 8th day. We have shown that, at this dose, the antiserum is mainly inhibiting the oestrogen surge. It is proposed that an LH surge precedes an oestrogen surge on Day 4 of pregnancy

Relative ability of ovine follicle stimulating hormone and its β-subunit to generate antibodies having bioneutralization potential in nonhuman primates

The relative ability of ovine follicle stimulating hormone and its β-subunit, two potential candidates for male contraceptive vaccine, to generate antibodies in monkeys capable of bioneutralizing follicle stimulating hormone was assessed usingin vitro model systems. Antiserum against native ovine follicle stimulating hormone was found to be highly specific to the intact form with no cross-reactivity with either of the two subunits while the antiserum against β-subunit of follicle stimulating hormone could bind to the β-subunit in its free form as well as when it is combined with α-subunit to form the intact hormone. Both antisera could block the binding of the hormone to the receptor if the hormone was preincubated with the antibody. However, the follicle stimulating hormone β-antisera could only inhibit the binding of the hormone partially (33% inhibition) if the antibody and receptor were mixed prior to the addition of the hormone, while antisera to the native follicle stimulating hormone could block the binding completely (100% inhibition) in the same experiment. Similarly antisera to the native follicle stimulating hormone was significantly effective in blocking (100%) response to follicle stimulating hormone but not the β-subunit antisera (0%) as checked using anin vitro granulosa cell system. Thus the probability of obtaining antibodies of greater bioneutralization potential is much higher if intact hormone is used as an antigen rather than its β-subunit as a vaccine

Assessment of luteal rescue and desensitization of macaque corpus luteum brought about by human chorionic gonadotrophin and deglycosylated human chorionic gonadotrophin treatment

The objective of the current study was to investigate the mechanism by which the corpus luteum (CL) of the monkey undergoes desensitization to luteinizing hormone following exposure to increasing concentration of human chorionic gonadotrophin (hCG) as it occurs in pregnancy. Female bonnet monkeys were injected (im) increasing doses of hCG or dghCG beginning from day 6 or 12 of the luteal phase for either 10 or 4 or 2 days. The day of oestrogen surge was considered as day '0' of luteal phase. Luteal cells obtained from CL of these animals were incubated with hCG (2 and 200 pg/ml) or dbcAMP (2.5,25 and 100 M) for 3h at 37°C and progesterone secreted was estimated. Corpora lutea of normal cycling monkeys on day 10/16/22 of the luteal phase were used as controls. In addition thein vivo response to CG and deglycosylated hCG (dghCG) was assessed by determining serum steroid profiles following their administration. hCG (from 15-90 IU) but not dghCG (15-90 IU) treatment in vivo significantly (P < 0.05) elevated serum progesterone and oestradiol levels. Serum progesterone, however, could not be maintained at a elevated level by continuous treatment with hCG (from day 6-15), the progesterone level declining beyond day 13 of luteal phase. Administering low doses of hCG (15-90 IU/day) from day 6-9 or high doses (600 IU/day) on days 8 and 9 of the luteal phase resulted in significant increase (about 10-fold over corresponding control P < 0.005) in the ability of luteal cells to synthesize progesterone (incubated controls) in vitro. The luteal cells of the treated animals responded to dbcAMP (P < 0.05) but not to hCC added in vitro. The in vitro response of luteal cells to added hCG was inhibited by 0,50 and 100% if the animals were injected with low (15-90 IU) or medium (100 IU) between day 6-9 of luteal phase and high (600 IU on day 8 and 9 of luteal phase) doses of dghCG respectively; such treatment had no effect on responsivity of the cells to dbcAMP. The luteal cell responsiveness to dbcAMP in vitro was also blocked if hCG was administered for 10 days beginning day 6 of the luteal phase. Though short term hCG treatment during late luteal phase (from days 12-15) had no effect on luteal function, 10 day treatment beginning day 12 of luteal phase resulted in regain ofin vitro responsiveness to both hCG (P < 0.05) and dbcAMP (P < 0.05) suggesting that luteal rescue can occur even at this late stage. In conclusion, desensitization of the CL to hCG appears to be governed by the dose/period for which it is exposed to hCG/dghCG. That desensitization is due to receptor occupancy is brought out by the fact that (i) this can be achieved by giving a larger dose of hCG over a 2 day period instead of a lower dose of the hormone for a longer (4 to 10 days) period and (ii) the effect can largely be reproduced by using dghCG instead of hCG to block the receptor sites. It appears that to achieve desensitization to dbcAMP also it is necessary to expose the luteal cell to relatively high dose of hCG for more than 4 days

Androgenic Response of Cultured Anthers and Microspores of Sorghum

Sorghum anthers with uninucleate microspores were dissected out of spikelets and plated on MS, B5 and N6 media with kinetin, NAA and 2,4-D. The callus induction frequencies (percentage of anthers plated) for the 3 hybrids tested were 60, 20-30 and 15-20%, for CSH 9, CSH 5 and CSH 12R, respectively. Uninucleate microspores showed the most cell division, and cultures of anthers at earlier (small cells without vacuoles) or later (binucleate microspores) stages did not show any androgenic response. The highest response was obtained at 26°C. After 12 d the anthers on all media, especially B5 and N6 started to turn black. Sucrose was more effective at inducing divisions (50-60% of microspores) than maltose (15-20%). Combined treatment with NAA and 2,4-D at 2.0 mg/litre induced divisions. When NAA and 2,4-D (2 mg/litre) were combined with kinetin at 0.05, 0.1, 0.15 and 0.2 mg/litre, the response increased, with a maximum number of multicellular microspores observed at 0.2 mg/litre kinetin. Microcalli that were growing within the anthers after 20-30 d of culture were transferred to MS medium; irregular masses of calli were observed after 30 d. It is concluded that sorghum microspore cultures may be less dependent on genotype or culture conditions than are anther cultures

Needed: a systems approach to improve flood risk mitigation through private precautionary measures

Private precautionary measures play an increasingly important role in flood risk management. The degree to which private precautionary measures mitigate flood risk depends mainly on the type of measure (and how effective it is) and how frequently and successfully it is implemented. These aspects are influenced by a complex interaction of physical and socio-economic processes, which makes the assessment and the prediction of the mitigation of flood risk via private precautionary measures a challenge. This paper provides an overview of factors and processes that influence the implementation and effectiveness of private precaution in mitigating flood risk, underpinning it with highlights from international examples. We recommend private precautionary measures for further use to improve flood risk mitigation, but stress that they need to be considered and implemented through a holistic systems approach to maximize their effectiveness

ARUBA: An Architecture-Agnostic Balanced Loss for Aerial Object Detection

Deep neural networks tend to reciprocate the bias of their training dataset. In object detection, the bias exists in the form of various imbalances such as class, background-foreground, and object size. In this paper, we denote size of an object as the number of pixels it covers in an image and size imbalance as the over-representation of certain sizes of objects in a dataset. We aim to address the problem of size imbalance in drone-based aerial image datasets. Existing methods for solving size imbalance are based on architectural changes that utilize multiple scales of images or feature maps for detecting objects of different sizes. We, on the other hand, propose a novel ARchitectUre-agnostic BAlanced Loss (ARUBA) that can be applied as a plugin on top of any object detection model. It follows a neighborhood-driven approach inspired by the ordinality of object size. We evaluate the effectiveness of our approach through comprehensive experiments on aerial datasets such as HRSC2016, DOTAv1.0, DOTAv1.5 and VisDrone and obtain consistent improvement in performance.Comment: Accepted to WACV 202

Alterations in sperm characteristics of follicle-stimulating hormone (FSH)-immunized men are similar to those of FSH-deprived infertile male bonnet monkeys

The quality of sperm ejaculated by bonnet monkeys and normal, healthy proven fertile volunteer men, both actively immunized with ovine follicle-stimulating hormone (oFSH), was examined at different times of study for chromatin packaging and acrosomal glycoprotein concentration by flow cytometry. Susceptibility of sperm nuclear DNA to dithiothreitol (DTT)-induced decondensation, as measured by ethidium bromide binding, was markedly high compared with values at day 0 in men and monkeys during periods when FSH antibody titer was high. Sperm chromatin structure assay yields alphat values, which is another index of chromatin packaging. Higher alphat values, signifying poor packaging, occurred in both species following immunization with heterologous pituitary FSH. The binding of fluorosceinated pisum sativum agglutinin (PSA-FITC) to acrosome of sperm of monkeys and men was significantly low, compared with values at day 0 (control) during periods when cross-reactive FSH antibody titer was high and endogenous FSH was not detectable. Blockade of FSH function in monkeys by active immunization with a recombinant oFSH receptor protein corresponding to a naturally occurring messenger RNA (mRNA) also resulted in production of sperm with similar defects in chromatin packaging and reduced acrosomal glycoprotein concentration. Thus, it appears that in monkeys and men, lack of FSH signaling results in production of sperm that exhibit defective chromatin packaging and reduction in acrosomal glycoprotein content. These characteristics are similar to that exhibited by sperm of some class of infertile men. Interestingly, these alterations in sperm quality occur well ahead of decreased sperm counts in the ejaculate