1 research outputs found
Skin Delivery of Hydrophilic Biomacromolecules Using Marine Sponge Spicules
We
report the development of sponge <i>Haliclona</i> sp.
spicules, referred to as SHS, and its topical application in skin
delivery of hydrophilic biomacromolecules, a series of fluorescein
isothiocyanate-dextrans (FDs). SHS are silicious oxeas which are sharp-edged
and rod-shaped (∼120 μm in length and ∼7 μm
in diameter). SHS can physically disrupt skin in a dose-dependent
manner and retain within the skin over at least 72 h, which allows
sustained skin penetration of hydrophilic biomacromolecules. The magnitude
of enhancement of FD delivery into skin induced by SHS treatment was
dependent on its molecular weight. Specifically, SHS topical application
enhanced FD-10 (MW: 10 kDa) penetration into porcine skin <i>in vitro</i> by 33.09 ± 7.16-fold compared to control group
(<i>p</i> < 0.01). SHS dramatically increased the accumulation
of FD-10 into and across the dermis by 62.32 ± 13.48-fold compared
to the control group (<i>p</i> < 0.01). <i>In vivo</i> experiments performed using BALB/c mice also confirmed the effectiveness
of SHS topical application; the skin absorption of FD-10 with SHS
topical application was 72.14 ± 48.75-fold (<i>p</i> < 0.05) and 15.39 ± 9.91-fold (<i>p</i> < 0.05)
higher than those from the PBS and Dermaroller microneedling, respectively.
Further, skin irritation study and transepidermal water loss (TEWL)
measurement using guinea pig skin <i>in vivo</i> indicated
that skin disruption induced by SHS treatment is self-limited and
can be recovered with time and efficiently. SHS can offer a safe,
effective, and sustained skin delivery of hydrophilic biomacromolecules
and presents a promising platform technology for a wide range of cosmetic
and medical applications