Population structure, long-term connectivity, and effective size of mutton snapper (Lutjanus analis) in the Caribbean Sea and Florida Keys

Genetic structure and average long-term connectivity and effective size of mutton snapper (Lutjanus analis) sampled from offshore localities in the U.S. Caribbean and the Florida Keys were assessed by using nuclear-encoded microsatellites and a fragment of mitochondrial DNA. No significant differences in allele, genotype (microsatellites), or haplotype (mtDNA) distributions were detected; tests of selective neutrality (mtDNA) were nonsignificant after Bonferroni correction. Heuristic estimates of average long-term rate of migration (proportion of migrant individuals/generation) between geographically adjacent localities varied from 0.0033 to 0.0054, indicating that local subpopulations could respond independently of environmental perturbations. Estimates of average longterm effective population sizes varied from 341 to 1066 and differed significantly among several of the localities. These results indicate that over time larval drift and interregional adult movement may not be sufficient to maintain population sustainability across the region and that there may be different demographic stocks at some of the localities studied. The estimate of long-term effective population size at the locality offshore of St. Croix was below the minimum threshold size considered necessary to maintain the equilibrium between the loss of adaptive genetic variance from genetic drift and its replacement by mutation. Genetic variability in mutton snapper likely is maintained at the intraregional level by aggregate spawning and random mating of local populations. This feature is perhaps ironic in that aggregate spawning also renders mutton snapper especially vulnerable to overexploitation

Effects of Hypoxia and Elevated Ammonia Concentration on the Viability of Red Snapper Embryos and Early Larvae

The effects of hypoxic conditions and elevated ammonia concentrations on the viability of embryos and newly hatched larvae of the red snapper (Lutjanus campechanus) were investigated. In all experiments, tested levels of hypoxia or ammonia concentrationswere applied to embryos and unfed newly hatched larvae from three different spawns. Exposures began at 1 h post fertilization (pf) and lasted until all individuals in a group had expired. Survival rates were monitored daily in duplicates for each spawn in each treatment. Fertilized eggs exposed to 2 mg L-1 dissolved oxygen (29% saturation) showed complete mortality before hatch while 81% of embryos in control groups (\u3e85% saturation) hatched and subsequently maintained high survival until 5 days pf. Exposure to a moderate hypoxia (target 3 mg L-1, 43% saturation) reduced significantly the hatch rate and subsequent survival rates; the magnitude of the difference in survival rate between control and exposed groups increased from 10% at hatch to 45% at 5 days pf. When oxygen concentration was maintained high (83% saturation) until 36 h pf and then progressively reduced to reach 3 mg L-1 at 2 days pf, the survival of exposed embryos and larvae did not differ significantly from those recorded in control groups, although potential delayed or cumulative effects of the treatment after 4 days pf could not be evaluated in this experiment. Embryos exposed to 10 mg L-1 total ammonia (TA-N), which corresponded to unionized ammonia (UIA-N) concentrations ranging between 0.307 and 0.468 mg L-1 in the conditions of the experiment, exhibited significantly reduced hatch rates and complete mortality between 3 and 4 days pf; the latter period corresponds to the onset of exogenous feeding of red snapper. In contrast, control groups (TA-N \u3c 0.26 mg L-1, UIA-N \u3c 0.006 mg L-1) maintained high survival rates beyond 5 days pf indicating potential to successfully initiate exogenous feeding. Exposure to 1 mg L-1 TA-N (0.020 mg L-1 \u3c UIA-N \u3c 0.054 mg L-1) did not alter significantly survival with respect to control groups. Significant interactions between the spawn and the tolerance to hypoxia or elevated ammonia were detected in both experiments, indicating that variations among spawns need to be accounted for when determining safe levels for hatchery production. Statement of relevance Achieving a reliable supply of high quality eggs and larvae is one of the main challenges of the developing marine aquaculture industry. Most studies to date have focused on maternal determinants of egg quality but the viability of embryos and newly hatched larvae can be impacted after fertilization if environmental conditions become unfavorable due to intensive hatchery conditions; this topic is poorly documented in marine fishes to date. This study provides data on the effects of two major stressors acting under high density culture (hypoxia and elevated ammonia concentration) on embryos and newly hatched larvae of the red snapper; the results highlight the importance to consider variations among spawns/parents when determining safe levels for hatchery production and also the high sensitivity of red snapper to these stresses, suggesting that this topic should be investigated in other marine offshore species. Relevance of the research to commercial aquaculture. The research contributes to control egg quality. (C) 2016 Elsevier B.V. All rights reserved

Antibiotic Resistance in a Coastal River in Mississippi, USA – Potential Drivers

Wastewater treatment plants (WWTPs) are major sources of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in water bodies. Most studies on the impact of WWTPs on antibiotic resistance have focused on freshwater systems, with little information on coastal and estuarine waters with variable salinity. This study monitored seasonal levels of ARGs at the effluent and downstream of the Pascagoula— Moss Point WWTP in the lower Pascagoula River, a coastal river in southeastern Mississippi, USA. Surface water samples were collected seasonally at upstream, outflow, and 3 downstream sites from February to November 2016. Bacterial resistance to sulfamethazine, tetracycline, and ciprofloxacin was quantified using selective culture and qPCR. Mixed—effects models were developed to identify potential driving factors of ARG concentrations related to the WWTP and local environmental conditions (salinity, water temperature, and pH). The best model was selected based on the lowest Akaike Information Criterion (AIC) corrected for small sample size. The results show that the genes sul1, sul2, and intI1 were detected, with intI1 having the highest relative concentration. The qPCR analysis suggests a negative relation between ARG levels and temperature and salinity. ARG concentrations peaked immediately downstream of the WWTP and decreased gradually further downstream in some months, but the spatial pattern varied widely between sampling months. The study highlights the complex patterns of environmental ARGs and the importance of accounting for the impact of WWTPs, local environmental factors, and other anthropogenic influences to understand their potential drivers

Population Structure, Long-Term Connectivity, and Effective Size of Mutton Snapper (\u3ci\u3eLutjanus analis\u3c/i\u3e) In the Caribbean Sea and Florida Keys

Genetic structure and average long-term connectivity and effective size of mutton snapper (Lutjanus analis) sampled from offshore localities in the U.S. Caribbean and the Florida Keys were assessed by using nuclear-encoded microsatellites and a fragment of mitochondrial DNA. No significant differences in allele, genotype (microsatellites), or haplotype (mtDNA) distributions were detected; tests of selective neutrality (mtDNA) were nonsignificant after Bonferroni correction. Heuristic estimates of average long-term rate of migration (proportion of migrant individuals/generation) between geographically adjacent localities varied from 0.0033 to 0.0054, indicating that local subpopulations could respond independently of environmental perturbations. Estimates of average long-term effective population sizes varied from 341 to 1066 and differed significantly among several of the localities. These results indicate that over time larval drift and interregional adult movement may not be sufficient to maintain population sustainability across the region and that there may be different demographic stocks at some of the localities studied. The estimate of long-term effective population size at the locality offshore of St. Croix was below the minimum threshold size considered necessary to maintain the equilibrium between the loss of adaptive genetic variance from genetic drift and its replacement by mutation. Genetic variability in mutton snapper likely is maintained at the intraregional level by aggregate spawning and random mating of local populations. This feature is perhaps ironic in that aggregate spawning also renders mutton snapper especially vulnerable to overexploitation

Sperm Repository For a Breeding Program of the Eastern Oyster \u3ci\u3eCrassostrea virginica\u3c/i\u3e: Sample Collection, Processing, Cryopreservation, and Data Management Plan

The Eastern oyster Crassostrea virginica (Family Ostreidae) is one of the most important fishery and aquaculture species in the U.S. and is a keystone species for coastal reefs. A breeding program was initiated in 2019 to support the fast-growing aquaculture industry culturing this species in the Gulf of Mexico. Oysters from 17 wild populations in embayment along the U.S. Gulf of Mexico coast from southwest Florida to the Matagorda Bay, Texas were used as broodstock for the program to maximize genetic diversity in the base population. A sperm repository of the broodstock was established to support the breeding project. The goal of this study was to demonstrate the sperm sample collection, processing, cryopreservation, and the data management plan involved in the establishment of a sperm germplasm repository of base populations. The supporting objectives were to: (1) develop a data management plan for the sperm repository; (2) streamline the procedure for sample collection, processing, and cryopreservation; (3) incorporate sperm quality analysis into the procedure, and (4) archive the cryopreserved samples as a repository for future use in the breeding program. This sperm repository included a total of 102 male oysters from the 17 collection sites (six oysters per site). A data management plan was developed with six categories, including sample collection, phenotype, fresh sperm, genotype, cryopreservation, and post-thaw sperm, as guide for data collection. Sperm collection was accomplished by strip spawn, and fresh sperm production, motility, and fertility were recorded for quality analysis. Cryopreserved sperm samples were sorted, labelled, archived, and stored in liquid nitrogen for future use. Post-thaw motility (1–30%) and plasm membrane integrity (15.34–70.36%) were recorded as post-thaw quality parameters. Overall, this study demonstrated a streamlined procedure of oyster sperm collection, processing, and cryopreservation for establishing a sperm repository that can serve as a template for construction of oyster germplasm repositories for breeding programs

When to discharge and when to voluntary or compulsory hospitalize? Factors associated with treatment decision after self-harm.

Clinicians assessing suicidal patients in emergency departments (EDs) must decide whether to admit the person to a psychiatric ward with voluntary or compulsory hospitalization or to discharge him/her as an outpatient. This cross-sectional study aimed to identify independent predictors of this decision among a large sample of self-harm (SH) patients. It used data from all patients admitted to four Swiss EDs between 2016 and 2019. Socio-demographic, clinical, and suicidal process-related characteristics data were evaluated against the decision for voluntary or compulsory hospitalization using t-tests, Chi-Square tests and logistic multiple regression. 2142 episodes from 1832 unique patients were evaluated. Independent predictors of decision to hospitalize included: male gender, advanced age, hospital location, depression and personality disorders, substance use, a difficult socio-economic condition, a clear intent to die, and a serious suicide attempt. Significant variables that emerged as independent predictors of compulsory hospitalization were hospital location, not having anxiety and personality disorders, being retired, having a clear intent to die, and making a serious suicide attempt. Hospital EDs had different rates of compulsory psychiatric admission. However, the decision to admit a patient for hospitalization, either voluntary or compulsory, was mainly based on clinical factors

Microsatellite multiplex panels for genetic studies of gray snapper (Lutjanus griseus) and lane snapper (Lutjanus synagris)

Microsatellites are codominantly inherited nuclear-DNA markers (Wright and Bentzen, 1994) that are now commonly used to assess both stock structure and the effective population size of exploited fishes (Turner et al., 2002; Chistiakov et al., 2006; Saillant and Gold, 2006). Multiplexing is the combination of polymerase chain reaction (PCR) amplification products from multiple loci into a single lane of an electrophoretic gel (Olsen et al., 1996; Neff et al., 2000) and is accomplished either by coamplification of multiple loci in a single reaction (Chamberlain et al., 1988) or by combination of products from multiple single-locus PCR amplifications (Olsen et al., 1996). The advantage of multiplexing micro-satellites lies in the significant reduction in both personnel time (labor) and consumable supplies generally required for large genotyping projects (Neff et al., 2000; Renshaw et al., 2006)

Projet « cabinets de groupe » : collaboration entre médecine de premier recours et psychiatrie [« Group medical practices » project : collaboration between primary care medicine and institutional public psychiatry]

Collaboration between primary care medicine and psychiatry is a well-known challenge. In order to improve access to psychological care for patients undergoing primary care, the « group medical practices » project proposes a collaborative care model in which a psychiatrist employed by a public psychiatric institution integrates group medical practices in order to provide assistance to primary care physicians. It is thus able to evaluate patients directly in the practices and to offer supervision and consilium spaces to primary care physicians

The rise and fall of the ancient northern pike master sex-determining gene

The understanding of the evolution of variable sex determination mechanisms across taxa requires comparative studies among closely related species. Following the fate of a known master sex-determining gene, we traced the evolution of sex determination in an entire teleost order (Esociformes). We discovered that the northern pike (Esox lucius) master sex-determining gene originated from a 65 to 90 million-year-old gene duplication event and that it remained sex linked on undifferentiated sex chromosomes for at least 56 million years in multiple species. We identified several independent species- or population-specific sex determination transitions, including a recent loss of a Y chromosome. These findings highlight the diversity of evolutionary fates of master sex-determining genes and the importance of population demographic history in sex determination studies. We hypothesize that occasional sex reversals and genetic bottlenecks provide a non-adaptive explanation for sex determination transitions