Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue-7

<p><b>Copyright information:</b></p><p>Taken from "Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue"</p><p>http://www.virologyj.com/content/4/1/133</p><p>Virology Journal 2007;4():133-133.</p><p>Published online 5 Dec 2007</p><p>PMCID:PMC2222636.</p><p></p>ere then collected and assayed for IL-8 by ELISA. IL-8 secretion from one experiment representative of three. Bars represent mean ± SD of triplicate cultures. B and C. After treatment of HFF with medium alone, LTA, Poly I:C, LPS, or CpG 2395 for 24 hours, cells were washed and CMVPT30-gfp was added. After four hours, the virus innoculum was removed and replaced with fresh culture medium. HCMV infection was quantified on day 10 post-infection by counting fluorescent (GFP expressing) cells in each well. B. Shown is a representative culture well from cells treated with medium alone. C. Percent inhibition compared to medium control. Results of one experiment, representative of 3 independent experiments, is shown. Bars represent mean ± SD of triplicate cultures. * indicates P ≤ 0.05 compared to control. ** indicates P ≤ 0.01 compared to control. *** indicates P ≤ 0.001 compared to control

Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue-0

<p><b>Copyright information:</b></p><p>Taken from "Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue"</p><p>http://www.virologyj.com/content/4/1/133</p><p>Virology Journal 2007;4():133-133.</p><p>Published online 5 Dec 2007</p><p>PMCID:PMC2222636.</p><p></p>ere then collected and assayed for IL-8 by ELISA. IL-8 secretion from one experiment representative of three. Bars represent mean ± SD of triplicate cultures. B and C. After treatment of HFF with medium alone, LTA, Poly I:C, LPS, or CpG 2395 for 24 hours, cells were washed and CMVPT30-gfp was added. After four hours, the virus innoculum was removed and replaced with fresh culture medium. HCMV infection was quantified on day 10 post-infection by counting fluorescent (GFP expressing) cells in each well. B. Shown is a representative culture well from cells treated with medium alone. C. Percent inhibition compared to medium control. Results of one experiment, representative of 3 independent experiments, is shown. Bars represent mean ± SD of triplicate cultures. * indicates P ≤ 0.05 compared to control. ** indicates P ≤ 0.01 compared to control. *** indicates P ≤ 0.001 compared to control

Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue-3

<p><b>Copyright information:</b></p><p>Taken from "Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue"</p><p>http://www.virologyj.com/content/4/1/133</p><p>Virology Journal 2007;4():133-133.</p><p>Published online 5 Dec 2007</p><p>PMCID:PMC2222636.</p><p></p>itional 24 period in one ml of fresh medium. These conditioned supernatants were collected and incubated in the presence of either medium as a control, rabbit polyclonal anti-IFNβ antibody, or normal rabbit serum for 1 hour at 37°C. Recombinant IFNβ (IFN) was also incubated in the presence of either medium as a control, rabbit polyclonal anti-IFNβ antibody, or normal rabbit serum for 1 hour at 37°C. The treated supernatants were then transferred to wells of confluent HFF fibroblasts and cultured for 24 hours. The conditioned medium was removed and the fresh HFF were challenged with CMVPT30-gfp. Fluorescent cells were then counted on day 10 post-infection (PI). The data shown is representative of 3 independent experiments. *** indicates P ≤ 0.001 compared to control

Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue-2

<p><b>Copyright information:</b></p><p>Taken from "Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue"</p><p>http://www.virologyj.com/content/4/1/133</p><p>Virology Journal 2007;4():133-133.</p><p>Published online 5 Dec 2007</p><p>PMCID:PMC2222636.</p><p></p>ected and tested for IFNβ by ELISA. The limit of detection of this assay was 1000 pg/ml. * indicates P ≤ 0.05 compared to control. ** indicates P ≤ 0.01 compared to control. *** indicates P ≤ 0.001 compared to control