Interferon regulatory factor 2 binding protein 2b regulates neutrophil versus macrophage fate during zebrafish definitive myelopoiesis

International audienceInterferon regulatory factor 2 binding protein 2b regulates neutrophil versus macrophage fate during zebrafish definitive myelopoiesis

Spatial accessibility of emergency medical services in Chongqing, Southwest China

BackgroundTimely access to emergency medical services (EMS) can significantly reduce mortality. In China, the evidence of the accessibility of complete EMS which considers two related trips and involves large rural areas is insufficient. This study aimed to explore the accessibility of ambulance services and complete EMS in Chongqing and its regional differences, and to provide a reference for improving spatial accessibility of EMS in Chongqing and optimizing allocation of EMS resources.MethodsThe nearest neighbor method was used to measure spatial accessibility of ambulance services and complete EMS. Spatial aggregation patterns and influencing factors of spatial accessibility of complete EMS were analyzed using Moran's I index, Pearson correlation and multiple linear regression.ResultsThe medians of shortest travel time for ambulance, monitoring ambulance, primary EMS and advanced EMS in Chongqing were 7.0, 18.6, 36.2, and 47.8 min. The shortest travel time for complete EMS showed significant spatial aggregation characteristics. The Low-Low types that referred to cluster of short EMS travel time mainly distributed in city proper. The High-High types that referred to cluster of long EMS travel time mainly distributed in northeast and southeast of Chongqing. Urbanization rate was a negative influencing factor on shortest travel time for primary EMS, while average elevation and the number of settlements were positive influencing factors. GDP per capita and urbanization rate were negative influencing factors on shortest travel time for advanced EMS, while the number of settlements was a positive influencing factor.ConclusionThis study evaluated the accessibility of EMS which considers two related trips in Chongqing. Although the accessibility of ambulances in Chongqing was relatively high, the accessibility of monitoring ambulance was relatively low. Regional and urban-rural differences in the accessibility of complete EMS integrating two related trips were obvious. It was recommended to increase financial investment in economic backward areas, increase high-quality EMS resources, enhance EMS capacity of central township health centers, strengthen road construction in mountainous areas, and provide reasonable planning of rural settlements for improving the spatial accessibility of EMS, narrowing the urban-rural gap and improving equity in getting EMS for all the people

Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases

The genus Coronavirus contains about 25 species of coronaviruses (CoVs), which are important pathogens causing highly prevalent diseases and often severe or fatal in humans and animals. No licensed specific drugs are available to prevent their infection. Different host receptors for cellular entry, poorly conserved structural proteins (antigens), and the high mutation and recombination rates of CoVs pose a significant problem in the development of wide-spectrum anti-CoV drugs and vaccines. CoV main proteases (M(pro)s), which are key enzymes in viral gene expression and replication, were revealed to share a highly conservative substrate-recognition pocket by comparison of four crystal structures and a homology model representing all three genetic clusters of the genus Coronavirus. This conclusion was further supported by enzyme activity assays. Mechanism-based irreversible inhibitors were designed, based on this conserved structural region, and a uniform inhibition mechanism was elucidated from the structures of M(pro)-inhibitor complexes from severe acute respiratory syndrome-CoV and porcine transmissible gastroenteritis virus. A structure-assisted optimization program has yielded compounds with fast in vitro inactivation of multiple CoV M(pro)s, potent antiviral activity, and extremely low cellular toxicity in cell-based assays. Further modification could rapidly lead to the discovery of a single agent with clinical potential against existing and possible future emerging CoV-related diseases

Etude tissulaire et moléculaire de la progression tumorale dans les lymphomes humains agressifs

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

Etude des facteurs de progression tumorale dans les lymphomes

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

Sequence and gene expression analysis of the agouti signalling protein gene in Rex rabbits with different coat colours

Rex rabbits have a unique fur structure with a variety of different coat colours. In this study, we analysed variability in the agouti signalling protein (ASIP) gene in Rex rabbits with different coat colours (castor, red, blue, chinchilla, otter and black). Three alleles at this locus were identified: A, light-bellied agouti (wild type), in castor and chinchilla Rex; at, black and tan, in otter, castor and chinchilla Rex; and a, black non-agouti, in black, red, blue, castor and chinchilla Rex rabbits. Two missense mutations, two synonymous substitutions and one indel were the identified polymorphisms associated to these three alleles, as already described by others. Gene expression of the ASIP gene was also evaluated in different tissues from animals of the six coat colours. Agouti signalling protein expression was always observed in all tissue/coat colour combinations

Sequence and gene expression analysis of the agouti signalling protein gene in Rex rabbits with different coat colours

Rex rabbits have a unique fur structure with a variety of different coat colours. In this study, we analysed variability in the <em>agouti signalling protein</em> (<em>ASIP</em>) gene in Rex rabbits with different coat colours (castor, red, blue, chinchilla, otter and black). Three alleles at this locus were identified: A, light-bellied agouti (wild type), in castor and chinchilla Rex; <em>a</em>^t, black and tan, in otter, castor and chinchilla Rex; and <em>a</em>, black non-agouti, in black, red, blue, castor and chinchilla Rex rabbits. Two missense mutations, two synonymous substitutions and one indel were the identified polymorphisms associated to these three alleles, as already described by others. Gene expression of the <em>ASIP</em> gene was also evaluated in different tissues from animals of the six coat colours. <em>Agouti signalling protein</em> expression was always observed in all tissue/coat colour combinations