Influence of humic acid and poultry manure on nutrient content and their uptake by T. aman rice

A study was conducted to examine the nutrient content and their uptake of rice as influenced by the application of humic acid (HA) and poultry manure (PM). This experiment was laid out in a Randomized Complete Block Design (RCBD) with three replications. Three levels each of humic acid (0, 3 and 6 L ha-1 ) and poultry manure (0, 3 and 6 t ha-1 ) were applied. BRRI dhan39 was used as test crop. Basal doses of N, P, K and S fertilizers were applied. Both humic acid and poultry manure either singly or in combination significantly affected the contents and uptake of N, P, K, S, Ca and Mg of BRRI dhan39. The maximum content of N, P, K, S and Ca except Mg and their uptake were recorded from the treatment combination of 6 L ha-1 humic acid along with 3 t ha-1 poultry manure

Plumieride fromAllamanda catharticaas an inhibitory compound to plant pathogenic fungi

Allamanda leaf extract (Allamanda cathertica) was made in water at room temperature (25 ± 2 °C) as well as in a number of less polar to highly polar solvents like methylene chloride, benzene, chloroform and ethyl acetate at their boiling point, that means, at refluxing temperature (40 ± 2 °C). Methylene chloride, benzene, chloroform, ethyl acetate and water extracts were applied to determine their growth inhibition against Phomopsis vexans, Phytophthora capsici, Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii. Results of these extracts showed that refluxing methanol, ethanol and ethyl acetate extracts of Allamanda were statistically similar for inhibition of mycelial growth of all fungi tested. But effect of 50% ethanol extract is different; it inhibited 100% mycelial growth of P. vexans, P. capsici and F. oxysporum; 83.33% of R. solani and 88.63% of S. rolfsii. Effort was also made to find out the compound in Allamanda to be responsible for such antifungal activity. Thin layer chromatography (TLC) of Allamanda extracts showed the presence of a number of compounds having polarity very high to low. The Rf values of compounds in 37-42 fractions were calculated and from these six fractions, crystals were separated. These crystals were more or less white. Melting point of these crystals was determined by ordinary and digital melting point apparatus that ranged from 145.5-162 C. Structural determination of the compound was done by Infra-red (IR) spectral study. The finger print region was 700-1400 cm-1. The strong band at 1612.4, 1633.6, 1693.4, 1655 and 2850.6 cm-1 indicated the presence of conjugated double bond (-C=C-C=C-), non-conjugated double-bond (-C=C-C-C-C=C-), carbonyl group attached to carbon-carbon double (-CO-C=C), ester (-COOR) and C-H stretching, respectively. Mass spectra of separated compounds gave molecular weight 470. All these characters are typical to pumieride as described previously. Again, In vitro screening of plumieride against P. vexans, P. capsici, F. oxysporum, R. solani and S. rolfsii were found effective in inhibiting radial mycelial growth of these fungi at 1:2 w/v concentration