Phytochemical Screening, Polyphenolic Content and Alpha-Glucosidase Inhibitory Potential of Leptadenia hastata (Pers.) Decne

Leptadenia hastata (Pers.) Decne. (Asclepiadaceae) is widely used as vegetable and traditionally in the management of diabetes mellitus and in the treatment of wounds and stomach ache. In this study, phytochemical screening, total phenolic contents and alpha-glucosidase activity of L. hastata leaf extracts were evaluated with the view to validating its antidiabetic potentials. Acetone, methanol and water extracts were screened for the polyphenolic contents while methanol and water extract were used for the evaluation of alpha-glucosidase activity. Phytochemical screening of L. hastata leaf indicated the presence of phenolic glycosides, tannins, flavonoids, proanthocyanidins, alkaloids and saponins. The total phenolics, total flavonoids and proanthocyanidins contents were in the ranges of 17-38, 10-16 and 4-10 mg/g respectively depending on the extraction solvent. The methanol and water extracts had 69.81 and 37.02 % inhibitory effect on alpha-glucosidase activity respectively. The results indicated that L. hastata leaf is rich in polyphenols and possess significant alpha-glucosidase inhibition potential and may therefore be a source of lead compounds in the management of diabetes mellitus and/or other diseases that may be caused by oxidative stress.Keywords: Leptadenia hastata, phytochemicals, polyphenols, alpha-glucosidase, effect, inhibitionNigerian Journal of Basic and Applied Science (2011), 19 (2): 181-18

Prevalence of HBsAg Among Prospective Blood Donors and Pregnant Women in Kebbi State, Nigeria

This study was aimed at determining the prevalence of Hepatitis B surface antigen (HBsAg) among prospective blood donors and pregnant women in Kebbi State, Nigeria. Three hundred and sixty one (361) apparently healthy prospective blood donors were recruited and screened for HBsAg using HBsAg rapid test kit. Those that tested positive for HBsAg (16.6%) were further assessed for liver function. The prevalence by gender was 23.4% and 13.1% for male and female subjects respectively. Prevalence by the three senatorial zones of the state is in the order Kebbi South (21.8%) > Kebbi North (15.1%) > Kebbi Central (13.6%). Subjects within the age group 26-30 years had the highest HBsAg prevalence of 20.9% compared to the other age groups. Single males have higher prevalence (26.1%) of HBV infection than their married counterparts (16.7%). Local government workers had highest prevalence (29.2%) followed by self-employed (27.6%) and then state government employees (14.3%). In females, occupational exposure (healthcare worker) and polygamy are risk factors with highest prevalence of 40.0% and 23.2% respectively. The levels of serum liver enzymes and bilirubin were significantly higher (p < 0.05) in HBsAg positive subjects compared to the HBsAg negative subjects. The reverse was, however, the case for albumin. Our result suggests that Kebbi State is one of the HBsAg hyper-endemic areas in Nigeria.Keywords: Hepatitis B, Prevalence, Liver function test, Kebbi State

Effect of Supplementation with a Locally Prepared Nutriceutical on Renal Function Profile in Alloxan-Induced Diabetic Rats

Diabetes mellitus is characterized by increased levels of marker of oxidative stress which play a role in the development of diabetes complications. Antioxidants are thought to be beneficial in curtailing the lipid peroxidation. In the current work, antioxidant- rich nutriceutic was formulated from onions, garlic, lemon, palm oil and crayfish (in ratio 6:6:2:1:5 respectively), which are known sources of vitamins A, C and E and Cu, Cr, Mn and Zn. The nutriceutic was administered to alloxan-induced diabetic rats for 3 weeks and the effect of the supplementation on renal function profile was studied. The results shows that supplementation significantly (P<0.05) reduced blood glucose, urea, creatinine and potassium. It also not significantly (P>0.05) reduced serum sodium and bicarbonate. The findings suggest that supplementation with naturally occurring antioxidant nutriceutic may reduce the risk of oxidative stress and complications associated with diabetes mellitus and might be beneficial in the routine treatment of diabetes mellitus patients.Keywords: Diabetes, nutriceutic, glucose, and renal function profileNigerian Journal of Basic and Applied Science (2011), 19 (2): 197- 20

Proximate composition and glycaemic index of destarched rice

There is strong evidence linking low glycaemic diets and reduced risk of developing diabetes mellitus (DM). This study was designed to develop low glycaemic index (GI) food using rice. Rice was subjected to enzymatic treatment at 40 °C for 8 mins for destarching. After the incubation, the proximate composition and GI of the processed rice were determined. Proximate analysis was carried out using the standard methods of AOAC, whereas GI was assessed using a postprandial oral glucose test. The results showed a significant reduction (p < 0.05) in percentage carbohydrates in the processed rice compared with the unprocessed control. Other parameters such as % moisture and lipids were also reduced in the processed rice when compared with the unprocessed control. Furthermore, the percentage of proteins and fibres were significantly increased in the processed rice compared to the unprocessed control. The GI of the processed rice was 69.86% as against the 86.43% of unprocessed rice, representing a 16.57% reduction. Overall, the results suggest that the enzymatic treatment of the rice have the potential of reducing both the starch content and GI of the rice. This can be explored in the development of a diabetic diet

Effect of Dried Lake Salt (Kanwa) on Lipid profile and Heart Histology of Female Albino Rats

Peripatum cardiomyopathy is a devastating form of cardiac failure affecting women mainly in their last month of pregnancy or early postpartum with high incidence in Northern Nigeria where the consumption of dried lake salt postpartum is high. The current work was designed to study the effect of dried lake salt on lipid profile and histology of heart in female albino rats. The rats were administered graded doses of the salt for 4 weeks. The group administered 300mg/kg body weight of the dried lake salt has significantly (P<0.05) lower high density lipoprotein-cholesterol as compared with the control. There was no significant (P>0.05) increase in low density lipoprotein-cholesterol but total cholesterol, triglyceride and very low density lipoprotein- cholesterol levels were lower compared to the control. Atherogenic index of the group administered 300mg/kg body weight was significantly (P<0.05) higher compared to the control. The histological examinations of section of the heart reveal chamber dilation, hypertrophy and focal atrophy. The study suggests that consumption of dried lake salt for 4 weeks caused alteration to heart tissue and may cause heart related diseases in rats.Keywords: Peripartum cardiomyopathy, Dried lake salt, Postpartum, Pregnanc

Effect of Copper, Manganese and Zinc With Antioxidant Vitamins on Pulse Rate and Lipid Profile of Salt-Loaded Albino Rats

Hypertension and dyslipidemia are associated with oxidative stress and are major causes of cardiovascular diseases amounting to 30% of global death rate. The effect of antioxidants supplementation on pulse rate and lipid profile in salt-loaded albino rats were investigated using a randomized control study with 30 albino rats divided into 5 experimental groups of 6 rats each. Groups 1 and 2 were normal untreated and salt-induced untreated respectively. Groups 3-5 were treated with Vitamins (A, C and E) with Cu, Mn and Zn respectively. Hypertension and dyslipidemia were induced using Salt-loading method (8% NaCl) for a period of five (5) weeks where Group 1 received normal rat feed and Groups 2-5 received salt-loaded diet. The heart rate of the rats was measured before and after the salt loading and dyslipidaemia was assessed at the end of the experiment. The results indicated that salt loading induced significant increase (

Possible Antidiabetic Mechanism of Action of Ex-maradi Okra Fruit Variety (Abelmoscus esculentus) on Alloxan Induced Diabetic Rats

Increasing evidences suggest that postprandial hyperglycemia plays an important role in the development of diabetes complications. Therefore, therapeutic approaches that act via lowering of postprandial glucose leading to reduce insulin requirement are currently very attractive. The present study investigated some possible mechanisms of hypoglycemic action of different parts (Whole Okra „WO‟; Okra Peel „OP‟ and Okra Seed „OS‟) of Ex-maradi Okra fruit variety in the management of diabetes mellitus in alloxan induced diabetic rats. Standard analytical procedures were employed in the study. The results indicated that various parts of Okra fruit have ability to stimulate glycogen synthesis in the liver and delay intestinal absorption of glucose with very significant glucose dialysis retardation index (GDRI) and high glucose adsorption capacity (GAC). Histological examination of the pancreatic tissue after administration of Okra fruit revealed evidence of pancreatic islets cells regeneration. These results suggest that the possible mechanisms of antidiabetic effect of Okra fruit are multidimensional and might involve increased glycogenesis, delay glucose absorption and pancreatic islets regeneration leading to increased insulin secretion. These might help control postprandial hyperglycemia in diabetes mellitus.Keywords: Diabetes, Glycogen, Glucose, Hyperglycemia, Okr

Optimization of thermoluminescence response of copper doped zinc lithium borate glass co-doped with Na2O

Establishing the basic procedures that will influence the enhancement of the TL yield of a phosphor is paramount in the issue of dosimetry. Melt quenching method was adopted in synthesizing lithium borate glass modified with ZnO, doped with CuO and codoped with Na2O. The structural and optical properties of zinc lithium borate and some TL properties of copper doped zinc lithium borate were reported in our previous works. The amorphous nature of the prepared glasses was confirmed by x-ray diffraction analysis (XRD). Physical properties of the glass were obtained via Archimedes principle. The copper doped zinc lithium borate was co-doped with different concentration of Na2O (0.025 mol % to 0.1 mol %). The glasses were irradiated with 4 Gy dose of gamma rays using 60Co gamma cell. The highest TL response was recorded against 0.05 mol% concentration of Na2O. The best settings for TLD reading of the proposed TLD were determined. The optimal annealing temperature and time for this composition was found to be 300 oC and 50 min respectively. The best heating rate at which the new TLD can be readout was 3 oC S-1

Oxidative stress among subjects with metabolic syndrome in Sokoto, North.Western Nigeria

Background: Oxidative stress is known to play a role in the pathophysiology of metabolic syndrome and its components. Racial differences may exist in the level of markers of oxidative stress and antioxidants in patients with metabolic syndrome.Aim: The aim of this study was to determine the oxidative stress and antioxidants status in subjects with metabolic syndrome in Sokoto, North.Western Nigeria.Methods: A cross.sectional community.based study was carried out. Two hundred subjects (96 males and 104 females) were recruited for the study using a multi.stage sampling technique. Demographic data were obtained from the participants. Evaluation of anthropometric variables, blood pressure, blood  glucose levels, lipid profiles, plasma insulin levels, total antioxidant status, and oxidative stress markers was performed.Results: The subjects with metabolic syndrome had significantly higher malondialdehyde as compared to those without metabolic syndrome (236.4 [92.2] vs. 184 [63.2] nmol/l). The antioxidant enzymes  (superoxide dismutase, glutathione peroxidase and catalase) were significantly lower in subjects with metabolic syndrome than in those without metabolic syndrome (11.3 [4.2] vs. 13.9 [4.1] U/ml, 160[42] vs. 220[32] U/ml, and 2.12 [0.2] vs. 2.42 [0.2] U/ml, respectively). Similarly, the antioxidant Vitamins (A, C, and E) levels were significantly lower in subjects with metabolic syndrome than in those withoutmetabolic syndrome (7.1 [4.1] vs. 7.7 [4.2] µmol/L, 225 [55.3] vs. 227.6 [62.3] µmol/L, and 75.9 [13.9] vs. 82.8 [18.6] mg/dl, respectively). There was a positive correlation between components of metabolic syndrome and free radicals.Conclusion: Significantly increased oxidative stress and diminished antioxidant defenses were found among Nigerians with metabolic syndrome.Key words: Antioxidants, metabolic syndrome, oxidative stres