2,773 research outputs found
Isotopic fractionation in proteins as a measure of hydrogen bond length
If a deuterated molecule containing strong intramolecular hydrogen bonds is
placed in a hydrogenated solvent it may preferentially exchange deuterium for
hydrogen. This preference is due to the difference between the vibrational
zero-point energy for hydrogen and deuterium. It is found that the associated
fractionation factor is correlated with the strength of the
intramolecular hydrogen bonds. This correlation has been used to determine the
length of the H-bonds (donor-acceptor separation) in a diverse range of enzymes
and has been argued to support the existence of short low-barrier H-bonds.
Starting with a potential energy surface based on a simple diabatic state model
for H-bonds we calculate as a function of the proton donor-acceptor
distance . For numerical results, we use a parameterization of the model for
symmetric O-H.... O bonds. We consider the relative contributions of the O-H
stretch vibration, O-H bend vibrations (both in plane and out of plane),
tunnelling splitting effects at finite temperature, and the secondary geometric
isotope effect. We compare our total as a function of with NMR
experimental results for enzymes, and in particular with an empirical
parametrisation , used previously to determine bond lengths.Comment: Final version, accepted for publication in Journal of Chemical
Physics. Minor changes, including more extensive discussion of relevant of
model to protein
Three-dimensional modelling on the hydrodynamics of a circulating fluidised bed
The rapid depletion of oil and the environmentalimpact of combustion has motivated the search for cleancombustion technologies. Fluidised bed combustion (FBC)technology works by suspending a fuel over a fast air inletwhilst sustaining the required temperatures. Using biomassor a mixture of coal/biomass as the fuel, FBC provides alow-carbon combustion technology whilst operating at lowtemperatures. Understanding the hydrodynamic processes influidised beds is essential as the flow behaviours causing heatdistributions and mixing determine the combustion processes.The inlet velocities and different particle sizes influence theflow behaviour significantly, particularly on the transitionfrom bubbling to fast fluidising regimes. Computationalmodelling has shown great advancement in its predictive capabilityand reliability over recent years. Whilst 3D modellingis preferred over 2D modelling, the majority of studies use2D models for multiphase models due to computational costconsideration. In this paper, two-fluid modelling (TFM) isused to model a 3D circulating fluidised bed (CFB) initiallyfocussing on fluid catalytic cracker (FCC) particles. Thetransition from bubbling to fast fluidisation over a rangeof velocities is explored, whilst the effects on the bubblediameter, particle distributions and bed expansion for differentparticle properties including particle sizes are compared. Dragmodels are also compared to study the effects of particleclustering at the meso-scale
Absolute Dynamical Limit to Cooling Weakly-Coupled Quantum Systems
Cooling of a quantum system is limited by the size of the control forces that
are available (the "speed" of control). We consider the most general cooling
process, albeit restricted to the regime in which the thermodynamics of the
system is preserved (weak coupling). Within this regime, we further focus on
the most useful control regime, in which a large cooling factor, and good
ground-state cooling can be achieved. We present a control protocol for
cooling, and give clear structural arguments, as well as strong numerical
evidence, that this protocol is globally optimal. From this we obtain simple
expressions for the limit to cooling that is imposed by the speed of control.Comment: 4 pages, Revetex4-1, 2 png figure
Coexistence of antiferrodistortive and ferroelectric distortions at the PbTiO (001) surface
The c(22) reconstruction of (001) PbTiO surfaces is studied by
means of first principles calculations for paraelectric (non-polar) and
ferroelectric ([001] polarized) films. Analysis of the atomic displacements in
the near-surface region shows how the surface modifies the antiferrodistortive
(AFD) instability and its interaction with ferroelectric (FE) distortions. The
effect of the surface is found to be termination dependent. The AFD instability
is suppressed at the TiO termination while it is strongly enhanced,
relative to the bulk, at the PbO termination resulting in a c(2x2) surface
reconstruction which is in excellent agreement with experiments. We find that,
in contrast to bulk PbTiO, in-plane ferroelectricity at the PbO termination
does not suppress the AFD instability. The AFD and the in-plane FE distortions
are instead concurrently enhanced at the PbO termination. This leads to a novel
surface phase with coexisting FE and AFD distortions which is not found in
PbTiO bulk
Photophysics of phycoerythrocyanins from the cyanobacterium Westiellopsis prolifica studied by time-resolved fluorescence and coherent anti-Stokes Raman scattering spectroscopy
Three building blocks of the antenna complexes of the cyanobacterium Westiellopsis prolifica were studied: PEC(X), which is similar to the α-subunit of phycoerythrocyanin (PEC), trimers of PEC and monomers derived from these by deaggregation with KSCN. The fit of the fluorescence decay curve of PEC(X) requires at least four exponentials, although it supposedly contains only one chromophore. The coherent anti-Stokes Raman scattering (CARS) spectra indicate that the heterogeneity observed is due to geometrical isomers, which are in part generated by photoinduced processes. A similar heterogeneity in chromophore structure and properties is also found in the monomers, where four exponentials are needed to fit the fluorescence decay curve. As in trimers, there is a long-lived, low-amplitude component, which can be assigned to impurities and/or oxidation products. The energy transfer time between the two phyocyanobilin chromophores in the β-subunit is about 500 ps; the lifetime of the fluorescing β-chromophore is 1.5 ns. The phycoviolobilin chromophore in the α-subunit adopts different geometries characterized by fluorescence lifetimes of about 240 and 800 ps. No evidence was found for energy transfer between the α-chromophore and the β-chromophores. This energy transfer occurs in trimers on a time scale of less than 20 ps; the energy transfer time between the two different types of β-chromophore is about 250 ps and the lifetime of the terminal emitter is about 1.5 ns. The excited state kinetics are therefore similar to those of PEC trimers from Mastigocladus laminosus, as are the CARS spectra, indicating a similar chromophore—protein arrangement. In comparison with phycocyanin, the ordering of the excited states of chromophores β84 and β155 may be changed. Although PEC trimers of Westiellopsis prolifica show almost as good a photostability as trimers of Mastigocladus laminosus, monomers are so photolabile that no CARS spectra could be recorded
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