Evaluation of hair growth promoting activity of petroleum ether extracts of Hemidesmus indicus Linn. (Seed) on Wistar Albino rats

In present investigation was carried out to screening of hair growth promoting potentiality of petroleum ether extract of Hemidesmus indicus Linn. (Seeds). Preliminary chemical tests and TLC analysis revealed the presence flavonoids and saponins. Hair growth promoting activity of petroleum ether of Hemidesmus indicus was screened by considering different parameters which included time taken for covering bald patch, length of hair produced, percentage of hair follicles in anagen and telogen phases, time of hair growth initiation and completion and level of minerals in blood. The petroleum ether extract of Hemidesmus indicus showed a very good hair growth promoting activity at a dose of 300 mg/kg which was comparable to that of 2% minoxidil. After 30 days of treatment with test and standard drugs it was observed that, time taken for covering the bald patch, hair growth initiation and completion time and quantitative hair growth were found to be comparable to that of the standard drug. An increase in percentage of hair follicles turning from telogen phase to anagen phase was noted. The control treated group of animals showed poor hair growth for all the parameters. Keywords:  Hemidesmus indicus Linn., Anagen, Catagen, Telogen, Minoxidil

Determination of salmeterol xinafoate and its degradation products according to ICH guidelines with use of UPLC technique

The objective of reducing analysis time and maintaining good efficiency, there has been substantial focus on high-speed chromatographic separations. Recently, commercially available ultra performance liquid chromatography (UPLC) has proven to be one of the most promising developments in the area of fast chromatographic separations. In this work, a new isocratic reverse phase chromatographic stability indicating assay method was developed using UPLC for salmeterol xinafoate bulk drug. A novel stability-indicating UPLC assay method was developed and validated for salmeterol xinafoate and its degradation products. An isocratic UPLC method was developed to separate the drug from the degradation products, using an Acquity UPLC BEH C18 (50 mm x 2.1 mm column). Mixture of methanol: 0.06 % and pH 3.4 ammonium acetate (65:35) was used as mobile phase. The flow rate was kept 0.6 mL/min and the detection was carried out at 228 nm. The linearity of the proposed method was investigated in the range of 10-50 μg/mL (r2 = 0.999) for salmeterol xinafoate. The method detection limit was 0.5 μg/mL and the method quantification limit was 1 μg/mL. The percentage recovery of salmeterol xinafoate was ranged from 97.2 to 99.5 %. The %RSD values for intra-day precision study were <1.0 % and for inter-day study were < 2.0 %, confirming that the method was sufficiently precise. The validation studies were carried out fulfilling International Conference on Harmonisation (ICH) requirements. The procedure was found to be specific, linear, precise (including intra and inter day precision), accurate and robust.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Evaluation of hair growth promoting activity of petroleum ether extract of Abrus precatorius Linn. on Wistar Albino rats

In present investigation was carried out to screening of hair growth promoting potentiality of petroleum ether extract of Abrus precatorius leaf. Preliminary chemical tests and TLC analysis revealed the presence flavonoids and saponins. Hair growth promoting activity of petroleum ether of Abrus precatorius was screened by considering different parameters which included time taken for covering bald patch, length of hair produced, percentage of hair follicles in anagen and telogen phases, time of hair growth initiation and completion and level of minerals in blood. The petroleum ether extract of Abrus precatorius showed a very good hair growth promoting activity at a dose of 300 mg/kg which was comparable to that of 2% minoxidil. After 30 days of treatment with test and standard drugs it was observed that, time taken for covering the bald patch, hair growth initiation and completion time and quantitative hair growth were found to be comparable to that of the standard drug. An increase in percentage of hair follicles turning from telogen phase to anagen phase was noted. The control treated group of animals showed poor hair growth for all the parameters. Keywords: Abrus precatorius Linn, Anagen, Catagen, Telogen, Minoxidi

Determination of salmeterol xinafoate and its degradation products according to ICH guidelines with use of UPLC technique

The objective of reducing analysis time and maintaining good efficiency, there has been substantial focus on high-speed chromatographic separations. Recently, commercially available ultra performance liquid chromatography (UPLC) has proven to be one of the most promising developments in the area of fast chromatographic separations. In this work, a new isocratic reverse phase chromatographic stability indicating assay method was developed using UPLC for salmeterol xinafoate bulk drug. A novel stability-indicating UPLC assay method was developed and validated for salmeterol xinafoate and its degradation products. An isocratic UPLC method was developed to separate the drug from the degradation products, using an Acquity UPLC BEH C18 (50 mm x 2.1 mm column). Mixture of methanol: 0.06 % and pH 3.4 ammonium acetate (65:35) was used as mobile phase. The flow rate was kept 0.6 mL/min and the detection was carried out at 228 nm. The linearity of the proposed method was investigated in the range of 10-50 μg/mL (r2 = 0.999) for salmeterol xinafoate. The method detection limit was 0.5 μg/mL and the method quantification limit was 1 μg/mL. The percentage recovery of salmeterol xinafoate was ranged from 97.2 to 99.5 %. The %RSD values for intra-day precision study were <1.0 % and for inter-day study were < 2.0 %, confirming that the method was sufficiently precise. The validation studies were carried out fulfilling International Conference on Harmonisation (ICH) requirements. The procedure was found to be specific, linear, precise (including intra and inter day precision), accurate and robust.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Study of forced degradation behaviour of eprosartan mesylate and development of validated stability indicating assay method by UPLC

The present research work describes comprehensive stress testing of eprosartan mesylate (EM) according to ICH guideline Q1A (R2), and development of a stability-indicating reversed phase ultra performance liquid chromatographic (UPLC) assay. The drug was subjected to acid (0.5N HCl), neutral and alkaline (0.5 N NaOH) hydrolytic conditions at 80 °C, and to oxidative decomposition at room temperature. Photolysis was carried out by exposing the drug during the day time to sunlight (60,000-70,000 lux) for two days and oxidative study was performed with 0.5 mg/ml in 30 % hydrogen peroxide (H2O2 ) at room temperature for 25 hr. The solid drug was also subjected to 50 °C for 30 days in a hot air oven. Degradation of the drug was found to occur under alkaline, acidic and neutral hydrolytic conditions. Separation of the drug and the degradation products was successfully achieved on a BEH (bridged ethylene hybrid) C18 column (1.7 µm, 2.1 mm × 150 mm) with gradient elution of water-acetonitrile as mobile phase. The flow rate and detection wavelength were 0.1 ml/min and 232 nm, respectively. The method was validated and the response was found to be linear in the drug concentration range 5-25 µg/ml (r2 = 0.999). The %RSD in intra-day and inter-day precision studies was < 0.8 %. Recovery of the drug from a mixture of degradation products was between 98.3 and 99.8 %. The LOD and LOQ of developed method were obtained at 0.15 µg/ml and 0.45 µg/ml respectively. The method was specific to the drug, selective to degradation products, and robust. PDA purity test also confirmed the specificity of the method.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Exploration of Baicalin analogues against main Protease involved in Covid-19 as potential inhibitors involving in silico approaches

The proteolytic enzymesinvolved in the processing and replication of coronavirus serve a promising drug targetsfor coronavirus. Although a few numbers of vaccines have been made available, there is a great need for effective treatment to manageit. The current study involved the exploration of baicalin analogues as potential inhibitor of viral proteolytic enzyme. Utilizing various computational tools (scaffold morphing, molecular docking and pharmacokinetic studies), the B74 and B86 analogueswere screened as potential inhibitors of main protease. Accordingly, these analogues can be explored further in the search of promising therapy against the coronavirus infection

Multi-Parametrized Optimized Ad-Hoc On Demand Distance Vector Routing

Abstract- Ad-hoc network is the infrasture less network which is created for the short duration. In MANET’s route is selected for the communication by the help of routing protocol. But in the path selection mechanism, no path is selected without any consideration of paratmeters. So, path is selected for the communication may not be the best for the communication. In MAODV, Multiple parameters are selected, to selecte the communication path by the help of which best path is selected for the communication. MAODV is the modified version Mobility impact in IEEE 802.11p infrastructureless vehicular networks in which parameters are number of nodes and mobility of nodes. In MAODV, parameters are bandwith, energy, mobility and number of nodes. Simulation has been done on NS-2 and performance is compared with the existing protocol to show the effectiveness of the protocol. Keywords-Routing, MANET,Multi-Parameter, AODV. I

Nonconvulsive Status Epilepticus on Electroencephalography: An Atypical Presentation of Subacute Sclerosing Panencephalitis in Two Children

Subacute sclerosing panencephalitis is a neurodegenerative disease secondary to measles infection that usually has a typical presentation with progressive myoclonia, cognitive decline, and periodic slow-wave complexes on electroencephalography. We report two pediatric cases who presented with periodic myoclonic jerks and cognitive decline. In both cases, the electroencephalogram showed continuous nonconvulsive status epilepticus activity. Both had elevated measles antibodies in cerebrospinal fluid and blood. Pediatricians need to be aware of this atypical presentation of subacute sclerosing panencephalitis

Association of three H - Hookworm, hemosuccus pancreaticus, and hypertension (portal) in a patient with melena

Hookworm infestations, endemic in India, are a common cause of iron deficiency anemia. Hemosuccus pancreaticus, a rare clinical condition, is due to passage of blood into the pancreatic duct possibly through a route between an aneurysm of an artery close to the pancreas and/or pancreatic duct, leading to gastrointestinal (GI) bleeding. Portal hypertensive upper GI bleed is also known since long. We report a case of a 38-year-old male with a history of alcoholism who was being investigated for GI bleeding who had concomitant hookworm infestation, hemosuccus pancreaticus as well as portal hypertension. To the best of our knowledge, this is the first report of common occurrence of hemosuccus pancreaticus and portal hypertension with hookworm infection. This case signifies the importance of infectious causes of GI bleeding to be considered even in cases where anatomic malformations or pathophysiological alterations are predominant