In vitro plant regeneration from Narbon Vetch (Vicia narbonensis L.) using cotyledonary node explants

Narbon vetch (Vicia narbonensis L.) is an agriculturally important forage plant that widely grows in an area extending from Central Europe to various parts of Asia. The study reports axillary shootregeneration from cotyledonary node explants obtained from, in vitro raised seeds of 4 - 5 and 14 - 15 days old seedlings on MS medium containing 2 - 6 mg/l kinetin-0.1 mg/l indole-3-butyric acid (IBA). Noshoot regeneration was recorded on 14 - 15 days old cotyledon node explants. Whereas, 4 - 5 days old cotyledon node explants showed high regeneration potential with the highest number of 3.85 shootsper explant, with mean shoot length of 2.11 cm and shoot regeneration frequency of 93.33%. The shoots obtained from all regeneration media could be easily rooted after pulse treatment with 50 mg/l IBA for 7min. All of which were morphologically normal and fertile and easily established under greenhouse conditions

In vitro regeneration of Turkish dwarf chickling (Lathyrus cicera L) using immature zygotic embryo explant

Dwarf chickling (Lathyrus cicera L) is an important forage legume crop. However, due to the presence of neurotoxin amino acid (b-N-oxalyl-L-a,b-diaminopropionic acid) in the dwarf chickling, its cultivationis limited. There is need to carry out studies for the development of varieties with reduced or none ofthis neurotoxin for beneficial and prolong consumption by animals. The study reports tissue cultureapproaches for multiplication of the plant using thidiazuron (TDZ), 6-benzylaminopurine (BAP), and #8733-naphthalene acetic acid (NAA) from immature zygotic embryos of the plant. The highest number (16.25)of shoots per explant was recorded on MS medium supplemented with 0.45 mg/l TDZ and 0.4 mg/lascorbic acid. Much reduced shoot regeneration was recorded on MS medium containing different combinations BAP+NAA+ascorbic acid. The TDZ-regenerated shoots were rooted by pulse treatmentwith 50 mg/l IBA for 7 min. The plantlets were successfully acclimatized under the greenhouse conditions

In vitro plant regeneration from Turkish Narbon Vetch (Vicia narbonensis L. var. narbonensis L.)

Narbon vetch (Vicia narbonensis L.) is an important forage species among vetches of central Europe, Mediterranean, Near East, Ethiopia, central Asia and India. The study reports in vitro micropropagation of narbon vetch using cotyledon node, shoot tip and zygotic embryo explants on MS medium containing 0.25, 0.50, 0.75 and 1.00 mg/l BAP. The highest number of 4.42 shoots was recorded on cotyledon node explants, which was followed by significantly reduced number of 3.40 and 3.54 shoots per explant on shoot tip and zygotic embryo with two cotyledons, respectively. Zygotic embryo with two cotyledons explant was recalcitrant and slow in regeneration compared to other explants but any concentration of BAP was sufficient for multiple shoot regeneration. Shoot length decreased statistically with increase in each concentration of BAP in the regeneration medium. 65.0% shoots rooted on MS medium containing 0.5 mg/l IBA and newly regenerated plants were acclimatized in the greenhouse