Protein landmarks for diversity assessment in wheat genotypes

Grain proteins from 20 Indian wheat genotypes were evaluated for diversity assessment based seed storage protein profiling on sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE). Genetic diversity was evaluated using Nei’s index, Shannon index and Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis by constructing dendrogram of fractions of proteins, which were used for the calculation of similarity coefficients between these varieties. Diversity analysis attributes exhibited the importance of seed storage as a marker system. The similarity ranged from 32.14% to as high as 100% between genotypes. Adoption of this technology would be useful to plant protection regulatory systems, especially for plant variety identification and registration of new plant varieties, breeding programs and protection purposes.Keywords: Sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), genetic diversity, population diversity index, coefficient of similarity.African Journal of Biotechnology Vol. 12(29), pp. 4640-464

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Not AvailablePlant–bacterial interactions in the rhizosphere are complex and determine the plant health, productivity and soil fertility. The Plant Growth Promoting bacterial strains were isolated from the rhizospheric soil of the cotton plant from various places of Haryana, India. One of the most promising isolate exhibiting maximum PGP traits was identified as Pseudomonas fluorescens based on the biochemical characterization, phenotypic microarray analysis and 16S rDNA sequencing. The isolate was initially screened on the basis of the seed germination assay and was then screened in vitro for their plant growth promoting traits like production of indoleacetic acid (IAA), ammonia production (NH3), hydrogen cyanide (HCN) production, siderophore production, phosphate solubilization, ACC deaminase activity and acetylene reduction activity (ARA). The isolate exhibited positive results for ammonia and siderophore production and phosphate solubilization. The isolate also showed acetylene reduction activity (ARA). The results indicated that P. fluorescens has significant potential when applied to soil–plant system to uncover its efficacy as effective PGPR.Not Availabl