New cytotoxic spirostane saponin and biflavonoid glycoside from the leaves of <i>Acacia saligna</i> (Labill.) H.L. Wendl.

<div><p>New naturally occurring spirostane saponin (25<i>S</i>)-5β-spirostan-3β-yl-3-<i>O</i>-β-d-xylopyranosyl(1 → 3)-<i>O</i>-β-d-xylopyranosyl(1 → 4)-β-d-galactopyranoside (<b>6</b>) and biflavonoid glycoside myricetin-3-<i>O</i>-rhamnoside (C7-<i>O</i>-C7) myricetin-3-<i>O</i>-rhamnoside (<b>4</b>) along with a series of known compounds erythrodiol (<b>1</b>), 3β-<i>O</i>-<i>trans</i>-<i>p</i>-coumaroyl-erythrodiol (<b>2</b>), quercetin-3-<i>O</i>-α-l-rhamnoside (<b>3</b>) and myricetin-3-<i>O</i>-α-l-rhamnoside (<b>5</b>) were separated from the leaves of <i>Acacia saligna</i> (Labill.), H.L. Wendl. Compounds <b>1</b> and <b>2</b> were separated for the first time from genus <i>Acacia.</i> The structures of compounds <b>1</b>–<b>6</b> were established on the basis of extensive 1D and 2D NMR spectroscopic techniques in combination with EI-MS and HR-ESI-MS. These compounds were screened for their antioxidant and cytotoxic activities against HEPG2 (liver cancer) cell line and significant results were obtained.</p></div

Evidence-based medicinal value of Rudbeckia hirta L. flowers

A phytochemical investigation on the 5-lipoxygenase (5-LOX) inhibitory methanolic extract of Rudbeckia hirta L. flowers yielded 10 phenolic metabolites, including three phenolic acids, two phenolic acid esters, four flavonol glycosides and a trimethylated flavonol. The structures of the isolated metabolites were determined on the basis of spectroscopic analyses and by comparison with the literature data. Seven of these metabolites were isolated for the first time from the genus Rudbeckia. The in vitro 5-LOX inhibitory, immunomodulatory and antioxidant (oxygen radical absorbance capacity) activities of the isolated compounds were evaluated, and the results provided a new scientific evidence for the ethnopharmacological use of the herb in inflammatory conditions

New <i>ent</i>-kaurane diterpenoid dimer from <i>Pulicaria inuloides</i>

<p>A new naturally occurring <i>ent</i>-kaurane diterpenoid dimer, 15β, 15′β-oxybis (<i>ent</i>-kaur-16-en-19-oic acid) (<b>1</b>) along with six known compounds, 15β-hydroxy-<i>ent</i>-kaur-16-en-19-oic acid (<b>2</b>), 15β-hydroxy-<i>ent</i>-kaur-16-en-19-oate-β-d-glucopyranoside (<b>3</b>), 6-hydroxykaempferol-3, 7-dimethyl ether (<b>4</b>), quercetagetin 3, 7, 3′-trimethyl ether (<b>5</b>), β-sitosterol (<b>6</b>) and β-sitosterol glucoside (daucosterol) (<b>7</b>) were isolated from the aerial parts of <i>Pulicaria inuloides</i> DC. Compounds <b>2</b>–<b>5</b> were isolated for the first time from genus <i>Pulicaria.</i> The structures of compounds <b>1</b>–<b>7</b> were established on the basis of extensive 1D and 2D NMR spectroscopic techniques in combination with ESI-MS. The antimicrobial activity of the isolated compounds was evaluated against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Candida albicans</i>. Sulphorhodamine B cytotoxic assay against HepG2 (liver cancer) cell line and ABTS antioxidant assay were carried out.</p

Evidence-based medicinal value of <i>Rudbeckia hirta</i> L. flowers

<div><p>A phytochemical investigation on the 5-lipoxygenase (5-LOX) inhibitory methanolic extract of <i>Rudbeckia hirta</i> L. flowers yielded 10 phenolic metabolites, including three phenolic acids, two phenolic acid esters, four flavonol glycosides and a trimethylated flavonol. The structures of the isolated metabolites were determined on the basis of spectroscopic analyses and by comparison with the literature data. Seven of these metabolites were isolated for the first time from the genus <i>Rudbeckia</i>. The <i>in vitro</i> 5-LOX inhibitory, immunomodulatory and antioxidant (oxygen radical absorbance capacity) activities of the isolated compounds were evaluated, and the results provided a new scientific evidence for the ethnopharmacological use of the herb in inflammatory conditions.</p></div