Forensic Analysis in Wildlife Crime Cases: Microscopy, DNA Profiling and Isotope Analysis

Illegal wildlife trade is one of the biggest threats to the environment and biodiversity. The growing volume of illegal trade in wildlife jeopardizes all the conservation efforts across the globe. Many species have become extinct due to the illegal wildlife trade and many have reached the verge of extinction. According to some estimates, the monetary values of the illegal wildlife trade are estimated to be several billion US dollars. To deal with wildlife crime cases, it becomes imperative to have a sound knowledge of the techniques required in the analysis of wildlife crime exhibits. In this chapter, we have outlined the three frequently used techniques in wildlife forensics viz. microscopy, DNA and isotope analysis for addressing the problems of species and individual identification, and additionally identification of the geographical origin of a wildlife sample. The basic essentials of these techniques have been discussed in this chapter

Dealing Wildlife Offences in India: Role of the Hair as Physical Evidence

India is known for its rich biodiversity, with a wide variety of wild floral and faunal species. This wildlife treasure of ours faces the threat of extinction due to rampant poaching and illegal trade. With most of the wildlife offence cases related to mammals having hair as physical evidence, it becomes imperative to use this evidence in the best possible way for wildlife crime investigation. We discuss the value of hair evidence with special reference to species characterization/identification using microscopic hair characteristics, keratin patterns, and mitochondrial DNA typing. The relevance of the techniques with respect to Indian scenario is specially taken care of and microscopic hair characteristics of one of the highly endangered species along with its keratin pattern are described. Finally, the use of mitochondrial DNA for species identification is also discussed

Evaluation of genetic polymorphisms at 21 autosomal STR loci in Ramgharia Sikh population of Punjab, India

Background STR (Short Tandem Repeat) markers are highly polymorphic markers, which are widely used in forensics DNA analysis and aid to ascertain unique genotype profiles of individuals and determine the genetic diversity of the given population. Aim In the present study, an attempt has been made to evaluate the population genetic diversity of the Ramgharia Sikh population of Punjab, India, using 21 autosomal STR loci (D3S1358, vWA, D16S539, CSF1PO, TPOX, D8S1179, D21S11, D18S51, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, SE33, D10S1248, D1S1656, D12S391, and D2S1338) to augment the emerging forensic database related to the indigenous population of India. Subjects and methods For generation of the database, 200 (blood on FTA card) samples were obtained from genetically unrelated Ramgharia Sikhs residing in the state of Punjab. Twenty-one autosomal STR markers were analysed using the Globalfiler® PCR amplification kit. Results With the help of various statistical tools, a total of 232 alleles were observed and 11.048 ± 1.284 (mean ± standard deviation) alleles per locus were recorded. No locus deviated from Hardy Weinberg Equilibrium. SE33 locus was found to be the most polymorphic and exhibited the highest discrimination power, that is, 0.99. Moreover, results further indicated that Ramgharia Sikhs of Punjab showed a high affinity with Bhils of Madhya Pradesh (India). Thus, the studied population showed genetic proximity with the geographically close populations of India and showed significant genetic variations with distant populations, which was evident from the UPGMA tree and Principal Component Analysis plot. Conclusion Overall, the 21 autosomal STRs were found to be polymorphic in the Ramgharia population and suitable for forensic casework and studies on population genetics

Genetic diversity of X-STR markers in Jat Sikh population of Punjab, India and its comparison with other 39 global populations

Background The Jat Sikh population is the largest endogamous group of Punjab, a state in north-west India, and has not yet been explored for genetic polymorphism based on X-STR genetic markers. In India, which is the second most populous country in the world, only two population studies based on X-STR markers have been reported so far. Aim To explore the genetic diversity of 12 X chromosomal STR genetic markers in the Jat Sikh population of Punjab and expand the X-STR polymorphism database. Subjects and methods In this study, a total of 200 Jat Sikh individuals (100 males and 100 females) residing in Punjab were investigated for 12 X-STR markers using the Investigator Argus X-12 QS Kit. Results The highest power of discrimination (PD) in females (PDf) and males (PDm) was observed to be 0.965 (DXS10135) and 0.929 (DXS10135 and DXS10148), respectively. DXS10135 was found to be the most polymorphic and discriminating locus among all the studied loci in both males and females with highest values of power of discrimination (PD) and polymorphic information content (PIC) as well. Conclusion Overall, the studied markers of the Argus 12 X-STR kit provide high polymorphic information which may prove to be an important tool in resolving issues such as missing person identification, incest, immigration disputes, kinship analysis and genealogical studies. The dataset obtained from this study will add to the present database of X-STRs