Oxidative stress pattern in hepatitis C patients co-infected with schistosomiasis

This study was designed to investigate the role of hepatitis C virus (HCV)-induced oxidative stress in the pathogenesis of the disease with the measurement of tumor necrosis factor (TNF-∝) and super oxide dismutase (SOD). Eighty patients from Hepatology Unit, Faculty of Medicine, Ain Shams University, were investigated. Thirty patients with bilharzial HCV and 30 patients with non-bilharzial HCV as compared to 20 healthy controls of the same age and sex ratio were investigated. The concentrations of liver enzymes [glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), alkaline phosphatase (ALP)], bilirubin (Bil), albumin (Alb) and total protein (TP) as well as TNF-α and Mn-SOD were determined. The mean level of the different liver function tests in the three groups in the study showed that the levels of GOT, GPT and ALP were significantly higher in the HCV groups as compared to the control group (p < 0.05). While serum bilirubin, albumin and total protein were non-significantly decreased in the HCV groups as compared to the control group (p > 0.05). Furthermore, the mean level of TNF-α was significantly higher in the HCV groups as compared to the control group (p < 0.001) and SOD was significantly decreased in the HCV groups as compared to the control group (p < 0.001). There is a cause-effect relationship between increased levels of TNF-α and decreased levels of SOD, relative to progression of chronic HCV, especially with bilharzias co-infection. Supporting the view that oxidative damage plays a role in chronic HCV infection, also TNF-α establishes a positive auto regulatory loop that can amplify the inflammatory response and lead to chronic inflammation. More evidence indicates that HCV block apoptosis and prolong survival of the host cell in order to gain time for replication and increase viral progeny production.Key words: Hepatitis C virus, tumor necrosis factor-alpha, superoxide dismutase, oxidative stress, schistosomiasis

Hepcidin and its Related Hematological Biomarkers of Anemia in Children on Hemodialysis: Role of Carnitine Deficiency

BACKGROUND: Anemia is one of the most common complications in end-stage renal disease (ESRD) patients. Hepcidin is a hormone that regulates iron homeostasis in patients with ESRD. Carnitine deficiency is commonly seen in hemodialysis (HD) patients. AIM: This study aimed to investigate the relationship between hepcidin and inflammatory and other anemia markers in children with ESRD and to evaluate the association of carnitine deficiency with anemia in these patients. SUBJECTS AND METHODS: Thirty pediatric patients with ESRD undergoing HD, and thirty healthy, age- and sex-matched children served as controls were included in the study. Serum levels hepcidin, iron status, high-sensitivity C-reactive protein, and total carnitine were measured. RESULTS: Statistically significant increases in serum levels of hepcidin (100.7 ± 0.99 ng\ml vs. 77.43 ± 0.8 ng\ml, p = 0.000), was found in HD children as compared to healthy controls. Statistically significant increase in serum levels of hs-CRP (3.94 ± 0.19 mg/l vs. 1.36 ± 0.07 mg/l, p = 0.04) was found in HD children as compared to healthy controls. However, serum levels of carnitine (29.59 ± 2.46 μmol/L vs. 36 ± 2.39 μmol/L, p = 0.000) showed statistically significant decreases in HD children as compared to healthy controls positive correlation was found between hepcidin and hs-CRP (r = 0.059, p = 0.042). Furthermore, a positive correlation was present between serum carnitine levels and serum iron levels (r = 0.651, p = 0.042). CONCLUSION: Serum hepcidin may be a more useful biomarker of functional iron deficiency in children on HD. The efficacy of carnitine treatment for children on HD with carnitine deficiency and its effect on anemia needs to be studied

Predictors of Serum 25-Hydroxyvitamin D Concentrations among a Sample of Egyptian Schoolchildren

Objective. To assess the level of 25-hydroxyvitamin D status among a sample of Egyptian schoolchildren and to evaluate predictors of deficiency and insufficiency. Subjects and Methods. A cross-sectional study comprising 200 prepubescent schoolchildren aged from 9 to 11 years was performed. A questionnaire including frequency of midday sun exposure, milk intake, physical activity, and level of maternal education was taken. Body mass index (BMI) was calculated; serum 25-hydroxyvitamin D [25(OH)D], serum calcium, phosphorus, and parathyroid hormone were measured. Results. Vitamin D deficiency [serum 25(OH)D < 20 ng/mL] was detected in 11.5% of subjects while its insufficiency (serum 25(OH)D is between 20 and 29.9 ng/mL) was detected in 15%. Results revealed that obesity, low physical activity, low sun exposure, and low maternal education level are significant predictors of insufficiency, though female gender, low maternal education level, and low milk intake are significant predictors of deficiency. Lower serum phosphorus and higher serum parathyroid hormone were significantly associated with both deficiency and insufficiency (p<0.05). Conclusion. Vitamin D deficiency and insufficiency are common among schoolchildren in Egypt. Food fortification, vitamin D supplementation, and increasing maternal awareness about the importance of physical activity and exposure of their children to ultraviolet light may help to overcome this problem

Prophylactic consequences of sodium salicylate nanoparticles in cisplatin-mediated hepatotoxicity

Abstract Unintended side effects linked to the antineoplastic drug cisplatin are a major drawback in its clinical application. The underlying source of these side effects include the generation of reactive oxygen species which are toxic and damaging to tissues and organs. In the present study the anti-inflammatory and antioxidant potential of sodium salicylate was assessed against cisplatin-induced hepatotoxicity in albino rats. Sodium salicylate was used as a model drug and loading into hollow structured porous silica using ultrasound-assisted sol–gel method to produce a nanoemulsion. Transmission Electron Microscopy and Dynamic Light scattering analysis were employed to assess the structural properties and stability of this model. Liver function was assessed by measuring biomarkers including ALT, AST & GGT and oxidant/antioxidant markers including MDA, NO, PON, GSH, MCP1 & AVP in serum or liver tissue. Additionally, blood leukocyte DNA damage was evaluated. Cisplatin significantly altered the normal levels of all biomarkers confirming its hepatotoxic effects. In contrast, treatment with sodium salicylate-loaded silica nanoemulsion significantly restored the levels of these markers. The finding suggests the protective effects of this model drug in preventing cisplatin-induced hepatotoxicity, and therefore may have implications in attenuating cisplatin-induced hepatotoxicity

Evaluation of DNA damage profile in obese women and its association to risk of metabolic syndrome, polycystic ovary syndrome and recurrent preeclampsia

Metabolic syndrome (MS) is a cluster of metabolic abnormalities. Obesity and MS are always accompanied by elevated oxidative stress which might affect cellular bio-molecules such as DNA. The aim of the present study is to investigate DNA damage profile in obese premenopausal women and its relation to the risk of MS, polycystic ovary syndrome (PCOS) and history of recurrent pre-eclampsia. The study included 90 obese women included cases with MS (n = 30), PCOS (n = 30) and previous history of recurrent preeclampsia (n = 30) and, age-matched healthy non-obese control women (n = 50). The assessment of leukocyte DNA damage was done by comet assay for all cases and controls. Anthropometry and biochemical parameters have been measured. Results showed that mean percent of DNA damage was significantly higher in MS, PCOS as well as in women with the recurrent preeclampsia as compared to healthy controls. The high level of mean DNA damage frequency in obese women was significantly associated with the increased number of metabolic syndrome components. Cases with 2, 3 and 3–5 components showed significantly higher levels of DNA damage than controls. Moreover, cases with 3–5 MS components showed significant higher DNA compared to those with the two components. Regarding PCOS, significant positive association between the mean frequency of DNA damage and waist circumference was observed. The study suggests that metabolic abnormalities, PCOS and recurrent pre-eclampsia might be contributed in development of DNA damage in obese women. DNA damage can serve as an early marker for obesity complications in premenopausal women. Keywords: DNA damage, Metabolic components, Obesity, PCOS, Recurrent preeclampsi

Association of plasma protein C levels and coronary artery disease in men

Several studies have shown the risk factor causes of coronary heart disease. In this study we tested the hypothesis that plasma protein C level might be used as a biomarker for coronary heart disease and myocardial infarction. The study included 60 men that were classified into 3 groups according to clinical examination; group I set as healthy control group, group II set as patients with ischemic heart disease and group III set as patients suffering from myocardial infarction. Different parameters were measured including, coagulation factor prothrombin time, partial thromboplastin time, fibrinogen and protein C. The activity of the cardiac enzymes (creatine phosphokinase, creatine phosphokinase-MB and lactate dehydrogenase) was also measured. Finally, lipids profile (total lipids, phospholipids, triacylglycerol, total cholesterol, low density lipoprotein cholesterone (LDL-C) and high density lipoprotein (HDL-C) were measured. The results demonstrate significant decrease level of protein C and prothrombin concentration (%) in ischemic heart disease and in myocardial infarction (MI) groups, when compared to the control group. Meanwhile, MI group showed more significant decrease comparing to IHD. Plasma protein C might serve as a marker for coronary artery disease in men. Further studies are warranted to bolster the data and to identify pathogenesis links between innate immune system activation and atherosclerosis.Keywords: Ischemic heart disease, myocardial infarction, protein C, coagulation factor, lipids profile. Afr. J. Biotechnol Vol. 12 No. 5