Prevalencija protutijela za protozoon Neospora caninum u goveda u provinciji Kerman u Jugoistočnom Iranu.

Neospora caninum is an intracellular parasite which causes abortion in cattle worldwide. The aim of this study was to determine the seroprevalence of Neospora caninum in cattle in the province of Kerman in South East Iran. Blood samples were collected from 285 cattle in the province of Kerman for determining the seroprevalence of Neospora caninum. A total of 285 serum samples were tested for anti-neospora antibodies. Serum samples were analyzed for antibodies against N. caninum antigen using a commercial N. caninum ISCOM ELISA kit. Antibodies to N. caninum were found in 36 of the 285 (12.6%) sera based on ELISA test results. This study is the first report of Neospora infection in this area. With regard to seropositivity, no significant difference was observed regarding origin, sex and age (P>0.05).Protozoon Neospora caninum je intracelularni parazit koji uzrokuje pobačaje u goveda diljem svijeta. Cilj istraživanja bio je odrediti seroprevalenciju neosporoze u goveda u području Kerman u Jugoistočnom Iranu. Ukupno je sakupljeno i pretraženo 285 uzoraka seruma. Uzorci su bili pretraženi komercijalnim testom N. caninum Iscom ELISA. Protutijela su pronađena u 36 od 285 uzoraka seruma (12,6%). U ovom je istraživanju prvi put dokazana prisutnost invazije vrstom Neospora caninum u pretraživanom području Irana. Seroprevalencija se nije značajno razlikovala s obzirom na podrijetlo pretraženih životinja, njihovu dob i spol (P<0,05)

Prevalencija protutijela za protozoon Neospora caninum u goveda u provinciji Kerman u Jugoistočnom Iranu.

Neospora caninum is an intracellular parasite which causes abortion in cattle worldwide. The aim of this study was to determine the seroprevalence of Neospora caninum in cattle in the province of Kerman in South East Iran. Blood samples were collected from 285 cattle in the province of Kerman for determining the seroprevalence of Neospora caninum. A total of 285 serum samples were tested for anti-neospora antibodies. Serum samples were analyzed for antibodies against N. caninum antigen using a commercial N. caninum ISCOM ELISA kit. Antibodies to N. caninum were found in 36 of the 285 (12.6%) sera based on ELISA test results. This study is the first report of Neospora infection in this area. With regard to seropositivity, no significant difference was observed regarding origin, sex and age (P>0.05).Protozoon Neospora caninum je intracelularni parazit koji uzrokuje pobačaje u goveda diljem svijeta. Cilj istraživanja bio je odrediti seroprevalenciju neosporoze u goveda u području Kerman u Jugoistočnom Iranu. Ukupno je sakupljeno i pretraženo 285 uzoraka seruma. Uzorci su bili pretraženi komercijalnim testom N. caninum Iscom ELISA. Protutijela su pronađena u 36 od 285 uzoraka seruma (12,6%). U ovom je istraživanju prvi put dokazana prisutnost invazije vrstom Neospora caninum u pretraživanom području Irana. Seroprevalencija se nije značajno razlikovala s obzirom na podrijetlo pretraženih životinja, njihovu dob i spol (P<0,05)

Contribution of food deprivation to the immune response in rainbow trout ( Oncorhynchus mykiss ) vaccinated against Cryptobia salmositica and Aeromonas salmonicida

The aims of the present study were to determine (a) the effectiveness of an attenuated live Cryptobia salmositica vaccine; (b) the effects of food deprivation on the immune response and its duration in rainbow trout ( Oncorhynchus mykiss ) immunised with a live C. salmositica vaccine or with a killed Aeromonas salmonicida vaccine. The fish were divided into three groups (I, II and III; 14 fish per group), those in Groups I and II were under food deprivation (0.40% of body weight), while Group III fish were fed to satiety. The study showed that the attenuated strain of C. salmositica did not cause anaemia and disease, and the fish were protected from clinical disease when they were challenged with virulent parasites. Parasitaemia in all fish vaccinated and challenged with virulent C. salmositica fluctuated and was relatively low; however, fish in Group III had higher parasitaemia than those in Groups I and II between weeks 8 and 14. The numbers of activated neutrophils increased [nitroblue tetrazolium (NBT) assay] after immunisation with both Cryptobia and Aeromonas vaccines and they remained high throughout the experiment. Antibody production (ELISA values) increased after vaccination and were slightly higher in Group III. ELISA titres against A. salmonicida increased after vaccination and decreased after 5 weeks. The titres increased again after the vaccinated fish were given booster, and they were higher than those in the first vaccinated fish

Prevalence of Zoonotic and Other Intestinal Protozoan Parasites in Stray Cats (Felis domesticus) of Kerman, South-East of Iran

Intestinal protozoan parasites constitute a major source of diseases for stray cats and have been recognized as important public health problems in several parts of the world. Considering the potential risk of stray cats for public health, present cross-sectional study was carried out to determine the type and frequency of protozoan parasites by faecal examination. A total of 100 stray cats were examined in Kerman city, Iran, Overall 67 cats (67%) were infected with at least one protozoan parasite. The following parasites, with their respective prevalence, were found; Isospora felis 38%, Isospora rivolta 25%, Toxoplasma gondii 16%, Sarcocystis spp. 8%, Cryptosporidium spp. 7%, and Giardia sp. 5%. Based on our data, the sex of stray cats was not significantly associated with the prevalence of gastrointestinal protozoan parasites. The high infection rate of zoonotic intestinal protozoan parasites in stray cats is considered to be critical from the viewpoint of public health importance.W

Seroprevalence of Neospora caninum Infection in Dairy Cattle in West of Iran

The Neospora caninum parasite causes abortion in cattle in virtually all parts of the world with enormous economic consequences. The purpose of this study was to determine the seroprevalence of antibodies of Neospora caninum in dairy cattle in Lorestan Province, west of Iran. A total of 347 dairy cows were randomly selected. The serum of each case was analyzed for the possibility of the presence of antibody against N. caninum antigen, using the commercial kit: ELISA. The results of the ELISA test indicated that from 347 dairy cattle examined, the antibodies to N. caninum were found in 34 (9.8%). The percentage of seropositive aborted cattle was 13.33%. This study also indicated that there was no significant relationship between seropositivity and such factors as the age, breed, and abortion history of the cattle. Moreover, no significant relationship between seroprevalence of infection among rural and industrial cows was found. The neosporosis could be one of the possible causes of abortion in cattle. Further studies are recommended to determine the relationship between this parasite and the occurrence of abortion in cattle in the province of Lorestan

Contamination of Soil Samples of Public Parks with Toxocara spp. Eggs in Kermanshah, Iran

Toxocariasis is a zoonotic helminth infection, occurring in humans by the accidental ingestion of embryonated eggs of Toxocara canis and less frequently Toxocara cati. The present study was conducted to determine the existence of Toxocara spp. eggs by using the sucrose flotation method. A total of 150 soil samples were collected randomly from 7 public parks in Kermanshah city between September and December 2014 for investigating the presence of infective stages of parasites and to determine the prevalence of helminth eggs. Of the 150 soil samples examined, 27 (18%) were infected with eggs of Toxocara spp. eggs. The present investigation showed that humans (especially children) from urban areas are at risk of acquiring helminth infection from contaminated soil. Since this host species is capable of transmitting zoonotic agents to both animals and humans, animal populations, including stray dogs and cats, have to be controlled to minimize the distribution of parasites