29 research outputs found
Therapeutic Approach and Characterisation of Watermelon (Citrullus lanatus) Rind against Acrylamide Toxicity
Watermelon (Citrullus lanatus) is a cheap and easily available fruit in the local markets of India. The rind, which is the outer layer of watermelon, is completely edible. It is the only fruit with 90 per cent of water, and is fully edible including its rind and seeds as they contains different types of nutrients which are needed by our body in day to day life. The benefits in our body, includes reduced blood pressure, presence of different types of vitamins, such as vitamin A, B & C, as well as different types of minerals required by our body. The present study aims in evaluating the presence of different secondary metabolites in the watermelon rind. The therapeutic efficacy of watermelon rind against acrylamide toxicity in the lymphocyte cell line is studied. As selenium is an important micronutrient, an attempt has been made to prepare the selenium nanoparticles followed by its characterisation
Expression profiling of cervical cancers in I ndian women at different stages to identify gene signatures during progression of the disease
Cervical cancer is the second most common cancer among women worldwide, with developing countries accounting for >80% of the disease burden. Although in the West, active screening has been instrumental in reducing the incidence of cervical cancer, disease management is hampered due to lack of biomarkers for disease progression and defined therapeutic targets. Here we carried out gene expression profiling of 29 cervical cancer tissues from I ndian women, spanning International Federation of Gynaecology and Obstetrics ( FIGO ) stages of the disease from early lesion (IA and IIA) to progressive stages (IIB and IIIA–B), and identified distinct gene expression signatures. Overall, metabolic pathways, pathways in cancer and signaling pathways were found to be significantly upregulated, while focal adhesion, cytokine–cytokine receptor interaction and WNT signaling were downregulated. Additionally, we identified candidate biomarkers of disease progression such as SPP 1, proliferating cell nuclear antigen ( PCNA ), STK 17A, and DUSP 1 among others that were validated by quantitative real‐time polymerase chain reaction ( qRT ‐ PCR ) in the samples used for microarray studies as well in an independent set of 34 additional samples. Integrative analysis of our results with other cervical cancer profiling studies could facilitate the development of multiplex diagnostic markers of cervical cancer progression. Cervical cancer is the leading cause of cancer deaths among women in I ndia, yet it remains poorly characterized at molecular level. This study provides one of the largest molecular profiling efforts from this region involving cervical cancer tissues from well‐defined clinical stages to identify molecular signatures of disease progression, as well as identify novel biomarkers distinguishing early and advanced disease. We expect this study to serve as a template for larger studies, including those based on high‐throughput sequencing, to help develop robust biomarkers of disease progression and potentially identify actionable therapeutic targets.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/101800/1/cam4152.pd
Effectiveness of ethylene glycol bis (2-aminoethyl ether) tetraacetic acid (EGTA) against cerium toxicity
876-883Therapeutic efficacy of EGTA (ethylene
glycol bis (2-aminoethyl ether) tetraacetic acid) against cerium intoxicated mice
was studied. Administration of cerium showed significant decrease in
haemoglobin percentage, RBC counts and blood glucose level with an increase in
the activity of serum transaminases and WBC counts. Decrease in the activity of
alkaline
phosphatase and glycogen content was noted
in liver and kidney after cerium exposure. Light and electron microscopical investigations
showed that these changes were recouped considerably with the administration of
EGTA suggesting its therapeutic efficacy against cerium toxicity
Reversal of Vanadium-induced Toxicity by Combination Therapy of Tiferron and α-tocopherol in Rat during Pregnancy and their Fetuses
Objective. The aim of this study was
to analyze the effect of tiferron (sodium 4, 5-dihydroxybenzene-1,
3-disulfonate) per se and combination with α-tocopherol against
vanadium induced developmental toxicity. Vanadium, as vanadyl sulphate
pentahydrate, was evaluated for embryotoxic/fetotoxic effect in
female albino rats (Sprague Dawley). Methods. The compound
was administered by gavage to pregnant animals at a dose of 15 mg/kg/day,
p.o. on day 6-15 of pregnancy (organogenesis). Tiferron was given
on day 16-18 as chelating agent. Cesarean sections were performed
on day 19 of gestation. Results. Maternal toxicity
was observed, the level of sugar in the blood decreased, while we
observed an increase in serum protein, serum alkaline phosphatase
and serum transaminase activity. Level of lipid peroxidation showed enhances
value in fetal and maternal liver. Vanadium induced inhibition in
glycogen contents. Protein contents were decreased in vital organs
where as increased in uterus and placenta. There was increased activity
of acid phosphatase with the concomitant decline in alkaline phosphatase,
adenosine triphosphatase and succnic dehydrogenase after vanadium intoxication.
Toxicant caused severe alteration in histopathological observation
of maternal and fetal liver, kidney, uterus and placenta proving
its toxic consequences at cellular level. Tiferron along with α-tocopherol
dramatically reversed alterations of all variables towards control
rather than individual treatment. Conclusion. The combination
therapy of tiferron and α-tocopherol played a beneficial role in
reducing vanadium induced developmental toxicity
Chelation therapy and vanadium: Effect on reproductive organs in rats
515-523Present investigation was planned to evaluate the therapeutic effectiveness of chelating agents against vanadium intoxication on blood and reproductive organs of rats. Male and female albino rats were injected vanadyl sulphate (7.5 mg/kg, po, for 21 days, 5 days in a week). Chelating agents tiron (T) alone and in combination with lipoic acid (LA), vitamin E (vit E) and selenium (Se) were given for 2 days/week. With the administration of vanadyl sulphate to rats fructose level in seminal vesicles was significantly (P0.05) declined. The activities of alkaline phosphatase and adenosine triphosphatase were also decreased, whereas glycogen content and acid phosphatase activity increased in testis, seminal vesicles, ovaries and uterus after toxicant exposure. Significant changes in serum transaminases, serum alkaline phosphatase and lactate dehydrogenase were recouped by chelation therapy. Lipid peroxidation, reduced glutathione level and triglycerides levels altered significantly after exposure to vanadium in rats. The ultrastructural damage in spermatogenic stages in treated animals showed recovery pattern after therapy. Co-treatment with antioxidants restored these activities. The most effective combination was tiron + selenium followed by tiron + vitamin E, and tiron + lipoic acid
Analysis of time-dependent recovery from beryllium toxicity following chelation therapy and antioxidant supplementation
798-802Efforts have been made to minimize the
toxic effect caused by beryllium. Adult cyclic rats of Sprague Dawley strain were
administered a bolus dose of 50mg/kg beryllium nitrate intramuscularly. The
chelation therapy with glutathione (GSH), dimercapto propane sulfonic acid (DMPS)+
selenium (Se) and D-Penicillamine (DPA) + Se was given for 3 days
followed by a rest of 1,3 and 7 days
respectively. The results revealed a significant fall in the blood sugar level,
serum alkaline phosphatase activity, serum proteins. A significant rise in the
transaminases i.e. aspartate aminotranferase and alanine aminotranferase
pattern is indicative of leakage of enzymes from liver resulting in alterations
in the cell permeability. A rise in the hepatic lipid peroxidation activity is
a direct indication of oxidative damage resulting in free radical generation. Results
of the distribution studies by atomic absorption spectrophotometry reveal an increased
concentration of beryllium in liver and kidney followed by lung and uterus. The
relative ability of 3 chelating agents to act as antagonists for acute
beryllium poisoning have been examined in liver, kidney, lungs and uterus. The
appreciable change in the beryllium concentration in various organs is
duration-dependent during the entire period being highly significant after 7
days rest. From the biochemical assays, and distribution studies it can be
assumed that DPA+Se was the most effective
therapeutic agent followed by DMPS+Se and
GSH. Thus it can be concluded that DPA+Se is a better therapeutic agent as compared
to DMPS+Se and GSH