Uterus transplantation in a sheep model: Novel surgical technique with long-term survival and uterus vitality. first case series in Argentina

Objective: To develop a sheep autologous uterus transplantation (UT) program with an innovative surgical technique and assess long term uterus vitality and animal survival. Methods: A novel surgical technique consisting of the procurement of the complete uterus and the two ovaries, back table vascular reconstruction, and subsequent implantation in the same animal, performing only two arterial and two venous anastomoses. Results: Four autologous transplantations were performed; anesthesia and surgery were well tolerated by all the animals without complications. Direct observation and Doppler US performed a week after UT and laparoscopy performed three months later confirmed uterus vitality. All animals were alive more than a year after transplantation. Conclusions: Our study was the first to describe a novel surgical technique for sheep uterus autologous transplantation in Latin America, showing long-term survival and uterus vitality.Fil: Maraschio, Martín A. Hospital Privado Universitario de Córdoba. Kidney and Pancreas Transplant Program; ArgentinaFil: Larcher, José M Sad. Hospital Privado Universitario de Córdoba. Department of Obstetrics and Gynecology; ArgentinaFil: Alcaraz, Alvaro. Hospital Privado Universitario de Córdoba. Kidney and Pancreas Transplant Program; ArgentinaFil: Giordano, Enzo. Hospital Privado Universitario de Córdoba. Kidney and Pancreas Transplant Program; ArgentinaFil: Reimondez, Santiago. Hospital Privado Universitario de Córdoba. Kidney and Pancreas Transplant Program; ArgentinaFil: Luján, Oscar. Universidad Nacional de Río Cuarto. Faculty of Agronomy and Veterinary. Department of Animal Clinic; ArgentinaFil: Iraci, Marcelo. Universidad Nacional de Río Cuarto. Faculty of Agronomy and Veterinary. Department of Surgical Pathologies; ArgentinaFil: Sereno, José L. Universidad Nacional de Río Cuarto. Hospital Escuela of the Faculty of Agronomy and Veterinary; ArgentinaFil: Priotto, Analía. Hospital Privado Universitario de Córdoba. Department of Anesthesiology; ArgentinaFil: Domínguez, Olga. Hospital Privado Universitario de Córdoba. Kidney and Pancreas Transplant Program; ArgentinaFil: Valle, Pablo. Hospital Privado Universitario de Córdoba. Department of Orthopedics and Traumatology; ArgentinaFil: Abrego, Maximiliano. Hospital Privado Universitario de Córdoba. Department of Obstetrics and Gynecology; ArgentinaFil: Rubio, Soledad. Hospital Privado Universitario de Córdoba. Department of Plastic and Reconstructive Surgery; ArgentinaFil: Lamberto, Cristian. Sanagec Clinic. Imaging Department; ArgentinaFil: Villada, Eduardo. Universidad Católica de Córdoba. Veterinary Hospital; Argentin

Body mass index and human sperm quality: Neither one extreme nor the other

The aim of the present study was to investigate the still contentious association between body mass index (BMI) and seminal quality. To this end, 4860 male patients (aged 18-65 years; non-smokers and non-drinkers), were classified according to BMI as either underweight (UW; BMI <20kgm-2; n≤45), normal weight (NW; BMI 20-24.9kgm-2; n≤1330), overweight (OW; BMI 25-29.9kgm-2; n≤2493), obese (OB; BMI 30-39.9kgm-2; n≤926) or morbidly obese (MOB; BMI ≥40kgm-2; n≤57). Conventional semen parameters and seminal concentrations of fructose, citric acid and neutral -glucosidase (NAG) were evaluated. The four parameters that reflect epididymal maturation were significantly lower in the UW and MOB groups compared with NW, OW and OB groups: sperm concentration, total sperm count (103.3±11.4 and 121.5±20.6 and vs 157.9±3.6, 152.4±2.7 or 142.1±4.3 spermatozoa ejaculate-1 respectively, P<0.05), motility (41.8±2.5 and 42.6±2.6 vs 47.8±0.5, 48.0±0.4 or 46.3±0.6 % of motile spermatozoa respectively, P<0.05) and NAG (45.2±6.6 and 60.1±7.9 vs 71.5±1.9, 64.7±1.3 or 63.1±2.1 mU ejaculate-1 respectively, P<0.05). Moreover, the percentage of morphologically normal spermatozoa was decreased in the MOB group compared with the UW, NW, OW and OB groups (4.8±0.6% vs 6.0±0.8%, 6.9±0.1%, 6.8±0.1 and 6.4±0.2%, respectively; P<0.05). In addition, men in the MOB group had an increased risk (2.3-to 4.9-fold greater) of suffering oligospermia and teratospermia (P<0.05). Both morbid obesity and being underweight have a negative effect on sperm quality, particularly epididymal maturation. These results show the importance of an adequate or normal bodyweight as the natural best option for fertility, with both extremes of the BMI scale as negative prognostic factors.Fil: Luque, Eugenia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Tissera, Andrea D.. Laboratorio de Andrología y Reproducción; ArgentinaFil: Gaggino, M. P.. Centro Integral de Ginecología, Obstetricia y Reproducc; ArgentinaFil: Molina, Rosa I.. Laboratorio de Andrología y Reproducción (lar); ArgentinaFil: Mangeaud, Adolfo. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Matemáticas. Cátedra de Estadística y Biometria; ArgentinaFil: Vincenti, Laura María. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Fisiología Humana; ArgentinaFil: Beltramone, Fernando. Centro Integral de Ginecología, Obstetricia y Reproducc; ArgentinaFil: Sad Larcher, José. Centro Integral de Ginecología, Obstetricia y Reproducc; ArgentinaFil: Estofán, Daniel. Centro Integral de Ginecología, Obstetricia y Reproducc; ArgentinaFil: Fiol De Cuneo, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Martini, Ana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentin