A DEEP INSIGHT ON DIABETIC NEUROPATHY: THE SILENT COMPLICATION OF DIABETES, WITH INPUTS ON ITS CAUSES, DIAGNOSIS, PATHWAYS, AND TREATMENTS

Background: The incidence of diabetic neuropathy (DNP) is a prominent complication for people suffering from diabetes. DNP is a common complication in patients suffering from diabetes, and it is affecting approximately more than 50% of the population where the nerves are affected by high glucose levels.Objective: The aim of the review paper is to inspect the complications, causes, diagnosis, pathogenesis, treatments, pathways, and management of DNP as all these factors play important role in the management of DNP. This paper also aims to identify the potential cures and the side effects if any associated with the commonly used treatments in conditions of DNP.Methods: The data collected for reviewing was by studying the published researchers from PubMed, Web of Science, Medline, Science Direct, Excerpta Medica Database, Cochrane, Elton B. Stephens Company (EBSCO), and Google open access publications from the year 1995–2017.Results: We have concluded on an interpretation that the drugs for treating DNP are managing the pain and controlling glucose levels but are reportedly causing major side effects. Hence, attention must be given to the potential risk factors for neuropathy and development of formulations with minimal side effects and a potential cure. We have focused on the recent researches, emerging problems, and techniques for identifying the patients suffering from DNP.Conclusion: The incidence of DNP is a prominent complication for people suffering from diabetes. Although the treatment available currently focusses on the pain management in DNP, attention must be given to the potential risk factors for neuropathy and development of formulations with minimal side effects and a potential cure

LYSOSOMAL MEMBRANE AND PROTEIN STABILIZATION BY DALBERGIA SISSOO (FAMILY: FABACEAE): IN VITRO ANTI-INFLAMMATORY ACTIVITY

Objective: Plants of the genus Dalbergia are reported to be useful in the treatment of arthritis, gonorrhoea and rheumatic pains. Present study was aimed to investigate the in vitro anti-inflammatory activity of ethanol extract from Dalbergia sissoo leaves (EDS) and to support its traditional use.Methods: EDS was investigated for it's in vitro anti-inflammatory activity in human red blood cell membrane stabilization (HRBC) method and protein denaturation method. Diclofenac sodium was used as the standard drug.Results: The EDS and diclofenac sodium showed a concentration dependent stabilization toward HRBC membrane with 314.3±0.01 and 34.91±0.01 µg/ml; 50% protection, respectively. EDS and diclofenac sodium also showed dose dependent protein denaturation with IC50 values 719.9±0.04 and 428.4±0.02 µg/ml, respectively.Conclusions: EDS possessed noticeable in vitro anti-inflammatory effect against the HRBC membrane stabilization method and denaturation of albumin. Further authoritative studies are necessary to make certain the mechanisms and constituents behind its anti-inflammatory actions.Â

Evaluation of Antidepressant Activity of Ethanolic Extract of Abies webbiana and Berberis aristata in Laboratory Animals

Abstract Objective: Abies webbiana and Berberis aristata is an herbal plant that has several therapeutic effects. It also heals depression, grief, nervous stress and tension. In the present study we evaluated anti-depressant effect of ethanolic extract from Abies webbiana and Berberis aristata by using Forced Swimming Test (FST) and Tail Suspension Test (TST). Methods: Two doses of ethanolic extract of Abies webbiana and berberis aristata (200 mg/kg and 400 mg/kg) was given orally. Immobility time were measured after 30 min after the dosing and compared with control group and Flouxetine (25mg/kg) as a standard group. Results: The ethanolic extract of BA and AW (400 mg/kg) was found to be effective and it exhibited activity similar to that of the conventional drug Flouxetine (25mg/kg) (p<0.001) whereas 200 mg/kg dose showed higher activity with significantly increased swimming time and suspension time and decreased immobility time than 400 mg/kg of ethanolic extracts and Flouxetine (25mg/kg). Conclusion: These results proposed 400 mg/kg of ethanolic extract was showed higher anti-depressant activity as compared to control which is similar to the standard

Syntheses and Pharmacological Activities of Isatin Derivatives

Twenty derivatives of isatin (1H-indole-2, 3-Dione) were synthesized by the schematic route as per as scheme. These were further characterized by spectroscopic analysis. All the novel synthesized derivatives of isatin were screened for their antimicrobial activity against Gram-positive bacteria: Staphylococcus aureus, Bacillus subtilis, Streptococcus pneumonia; Gram-negative bacteria: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and fungal strains: Candida albicans, Aspergillus niger, Penicillium chrysogenum, Rhizopus oryzae by cup-plate method. Results of antimicrobial screening showed that compound ID5, ID9, ID15 and ID18 possess potent antibacterial activity. Whereas ID1, ID3, ID4, ID7 and ID20 possess antifungal activity comparable to standard drugs. Keywords: Isatin; Antimicrobial; Antibacterial; Antifungal

Effect of <i>Delonix regia</i> (Boj. Ex Hook.) Raf. stem bark extract against experimentally induced ulcers in rats

49-54Delonix regia, commonly called Flame Tree or Flamboyant (locally, Gul Mohor) is a common tree traditionally used to treat various diseases like gastric problems, body pain, rheumatic pains of joints and wound healing. Here, we carried out biological profiling of Delonix regia as antiulcer agent. Antiulcer activity of the ethanol extract from stem bark was evaluated on pylorus ligation and indomethacin induced ulcer in Wistar albino rats. Ethanol extract from stem bark of D.regia was administered at the doses 100, 200 and 400 mg/kg/day, p.o. for 7 days. Ulcer index, gastric pH, volume, free acidity, total acidity, total carbohydrate (TC), protein (P), mucin content (TC/P) and gastric mucus were evaluated in pylorus ligation model, while ulcer index, malondialdehyde, GSH, PGE2, and gastric mucus were estimated in the indomethacin induced ulcer model. Ex vivo assay for the activity of H+/K+-ATPase was also done. The results showed significant inhibition on H+/K+-ATPase in a dose dependent manner and comparableto their respective positive control group of rats demonstrating that ethanol extract of stem bark of Delonix regia possesses significant antiulcer properties

Acute, sub-chronic oral toxicity studies and evaluation of antiulcer activity of Sooktyn in experimental animals

Sooktyn (SKN), mineralo-herbal drug which is being used largely by the patients for its extremely good therapeutic value to treat the gastric ulcers. The present study was undertaken to evaluate the toxicity studies and antiulcer activity of SKN. Acute and sub-chronic toxicities were studied in male and female Wistar rats. A single acute SKN of 2 000 mg/kg was administered by oral gavage for acute toxicity. Sub-chronic doses were 400 and 800 mg/kg/day. The major toxicological end points examined included animal body weight and food intake, selected tissue weights, and detailed gross necropsy. In addition, we examined blood elements: hematocrit, hemoglobin concentration, erythrocyte count, total leukocyte count and MCH, MCHC and platelets as well as biochemical parameters: urea, sugar, alanine transaminase, aspartate transaminase, alkaline phosphatase, total proteins, and creatinine. Also, anti-ulcer activity was carried out by employing indomethacin, ethanol, pylorus ligation, and hypothermic-stress-induced ulcer models. LD 50 may be greater than 2 000 mg/kg (orally) for SKN and there were no signs of toxicity on 28 days sub-chronic oral administration of 400 and 800 mg/kg of SKN in rats on the basis of blood elements and biochemical parameters. The ulcer indices decrease in all ulcer models with 66.62%, 61.24%, 80.18%, and 74.76% in indomethacin, ethanol, pylorus ligation, and hypothermic-stress-induced ulcer models, respectively. The results suggest that SKN has no signs of toxicity at 2 000 mg/kg body weight of rats orally; sub-chronically. The drug is safe and has antiulcer activity

Biological activities and medicinal properties of Cajanus cajan (L) Millsp.

Cajanus cajan (L) Millsp. (Sanskrit: Adhaki, Hindi: Arhar, English: Pigeon pea, Bengali: Tur) (family: Fabaceae) is the most important grain legume crop of rain-fed agriculture in semi-arid tropics. It is both a food crop and a cover/forage crop with high levels of proteins and important amino acids like methionine, lysine and tryptophan. During the last few decades extensive studies have been carried out regarding the chemistry of C. cajan and considerable progress has been achieved regarding its biological activities and medicinal applications. This review article gives an overview on the biological activities of the compounds isolated, pharmacological actions and clinical studies of C. cajan extracts apart from its general details