25 research outputs found

    Independent of DAZL-T54A variant and AZF microdeletion in a sample of Egyptian patients with idiopathic non-obstructed azoospermia

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    Mohammed M El Shafae,1 Jehan H Sabry,1 Eman G Behiry,1 Hanan H Sabry,2 Mona A Salim,1 Alaaeldin G Fayez3 1Department of Clinical and Chemical Pathology, Benha Faculty of Medicine, Benha University, Benha, Egypt; 2Department of Dermatology, Venereology and Andrology, Benha Faculty of Medicine, Benha University, Benha, Egypt; 3Department of Molecular Genetics and Enzymology, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt Background: The microdeletion events that occur in the Y chromosome-azoospermia factor (AZF) region may lead to dyszoospermia. Also, the deleted azoospermia (DAZ) gene on AZFc and autosomal deleted azoospermia like gene (DAZL) are suggested to represent impairment, so it is interesting to determine the independency pattern of the AZF region and DAZL gene in azoospermic patients. Aim: To study the molecular characterization of AZFc and DAZL in 64 idiopathic non-obstructed azoospermia patients and 30 sexually reproductive men. Methods: SYBR Green I (Q-PCR) and AZF-STS analysis was used for DAZ gene, and SNV-PCR and confirmative Sanger sequencing for DAZL gene. Results: The present study observed that 15.6% had AZFc microdeletion, out of which 10% had DAZ1/2 deletion, and no T54A variant in the DAZL gene was found. Conclusion: In the current work, the novelty is that spermatogenic impairment phenotype, present with AZFc microdeletions, is independent of the T54A variant in the DAZL gene, and AZFc microdeletions could be a causative agent in spermatogenic impairment. Keywords: male infertility, azoospermia, AZF, DAZL, deletio

    Removal of Reactive Blue Dye 19 from Textile Wastewater by Elctrocoagulation Using Iron Electrodes

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    ABSTRACT This work deals with the possibility of using elctrocoagulation method for removal of reactive blue 19 dyes from textile industry wastewater. The effect of various operational parameters of the removal efficiency were investigated and optimized. The removal of dye using iron sacrificial anode was affected by the initial pH, the current density, the amount of sodium chloride and initial dye concentration. The removal efficiency reaching 99.6% at optimum operating conditions of pH 11.5, current density of 50mA/cm 2 , electrolysis time of 10mins, 100mg/l initial dye concentration, 3g/l sodium chloride as supporting electrolyte, and 25 0 C. Cyclic voltametry and linear polarization have been used to determine the electrochemical characteristics of the treated dye solutions and the process which occurred at the anode surfaces
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