The Development of a Nutrition Screening Tool for Mental Health Settings Prone to Obesity and Cardiometabolic Complications: Study Protocol for the NutriMental Screener

People living with serious mental illness (SMI) experience physical health complications at disproportionate rates to people without an SMI. Unhealthy dietary intake and disordered eating behaviors are key driving factors. There is a lack of valid nutrition-risk screening tools targeted to mental health services, and typically used nutrition-risk screening tools are not suitable for mental health services. This paper details the rationale and study protocol for development and validation of the NutriMental screener, a tool for use in clinical practice to identify service users who are at risk for common nutrition issues experienced by this population group and trigger referral to a specialist clinician. The development process includes five phases. Phase I is the development of nutrition-related domains of interest from screening tools used in mental health services. Phase II involves a literature review and service-user interviews to identify additional domains. Phase III consists of international workshops with relevant clinicians and persons with SMI to gain a consensus on questions to be included in the draft tool. Phase IV involves conducting multinational feasibility and preliminary validation studies. Phase V consists of performing formal validation studies. The development of a nutrition-risk screening tool for mental health services is a necessary step to help rectify the physical-health disparities and life-expectancy gap for people with SMI

Everolimus in Combination with Cyclosporin A as Pre- and Posttransplantation Immunosuppressive Therapy in Nonmyeloablative Allogeneic Hematopoietic Stem Cell Transplantation

Everolimus (RAD001) is an mTOR inhibitor that has been successfully used as an immunosuppressant in solid-organ transplantation. Data in allogeneic hematopoietic stem cell transplantation (HSCT) is limited. This study aimed to investigate pharmacokinetics, safety, and efficacy of RAD001 in a canine allogeneic HSCT model. First, pharmacokinetics of RAD001 were performed in healthy dogs in order to determine the appropriate dosing. Doses of 0.25 mg RAD001 twice daily in combination with 15 mg/kg cyclosporin A (CsA) twice daily were identified as appropriate starting doses to achieve the targeted range of RAD001 (3-8 μg/L) when orally administered. Subsequently, 10 dogs were transplanted using 2 Gy total body irradiation (TBI) for conditioning and 0.25 mg RAD001 twice daily plus 15 mg/kg CsA twice daily for pre- and posttransplantation immunosuppression. Seven of the 10 transplanted dogs were maintained at the starting RAD001 dose throughout the study. For the remaining 3 dogs, dose adjustments were necessary. RAD001 accumulation over time did not occur. All dogs initially engrafted. Five dogs eventually rejected the graft (weeks 10, 10, 13, 27, and 56). Two dogs died of pneumonia (weeks 8 and 72) but were chimeric until then. Total cholesterol rose from median 4.1 mmol/L (3.5-5.7 mmol/L) before HSCT to 6.0 mmol/l (5.0-8.5 mmol/l) at day 21 after HSCT, but remained always within normal range. Changes in creatinine and triglyceride values were not observed. Long-term engraftment rates were inferior to sirolimus/CsA and mycophenolate mofetil (MMF)/CsA regimen, respectively. RAD001/CsA caused a more pronounced reduction of platelet counts to median 2 × 109/L (range: 0-21 × 109/L) and longer time to platelet recovery of 21 days (range: 14-24 days) compared with MMF/CsA. CsA c2h levels were significantly enhanced in the RAD001/CsA regimen, but c0h and area under the curve from 0 to 12 hours (AUC0-12h) values did not differ compared with an MMF/CsA immunosuppression. In summary, immunosuppression consisting of RAD001 and CsA is well tolerated but not as efficient as with other established immunosuppressants in a canine nonmyeloablative HSCT regimen. Hence, our study does not support the application of RAD001/CsA as standard practice in this setting

FOTONARRATIVA: ESCREVENDO COM LUZ E PALAVRAS

Narrar sempre foi uma necessidade intrínseca ao ser humano e, ao longo dos tempos, foram utilizadas diversas formas de satisfazê-la. A narração implica o registro das memórias, a organização e ressignificação dos fatos no sentido temporal e espacial, além da construção social do sujeito-autor. Nesse sentido, o presente trabalho busca estabelecer um diálogo consistente entre fotografia e palavra a partir do pressuposto de que ambos são reconhecidos como textos, ainda que apresentem linguagens distintas. O projeto divide-se em três etapas a partir das quais se discute: a) os aspectos teóricos acerca do campo fotográfico e seus desdobramentos, b) processos de escrita, reescrita e correspondências entre a linguagem verbal e fotográfica e, c) a constituição de alguns gêneros textuais como resultados de situações narrativas. Ao longo dos encontros, busca-se atrelar a teoria e a prática a partir da execução de tarefas que têm como objetivo pensar a fotografia e o texto escrito a partir do ponto de vista narrativo e provocando uma expansão para o campo artístico

PSICANÁLISE: O DESEJO ONLINE

O presente projeto intitulado “Psicanálise: o desejo online” busca, de forma generalizada, dialogar e expor sobre a necessidade ou o vício da internet atualmente, por meio dos conceitos psicanalistas e principalmente do desejo. Para isso, dividimos o mesmo em três partes: a teoria, a pesquisa e a prática. A primeira é composta por estudos dos fundamentos teóricos psicanalíticos, além da pesquisa sobre a vida e conceitos de Sigmund Freud. Partindo do conceito freudiano de desejo, resolvemos levantar dados sobre o uso de internet dos alunos do campus e, questionários foram distribuídos para três turmas. Após feito o tratamento dos dados obtidos, estes foram aplicados em gráficos e comparados entre turmas e entre sexo, sempre com base psicanalítica, concluindo, assim, a segunda parte. A terceira parte, enfim, contém parte prática que envolve os fundamentos informáticos na elaboração de um site que conta com a exibição dos produtos do projeto, gráficos e pesquisas. Além disso, um quiz foi desenvolvido com o intuito de indicar para o usuário seu perfil de viciado, zen ou mediano, de acordo com suas respostas. A internet, mais do que o maior meio de comunicação atual, também é, um meio onde evitamos a comunicação! Evitar comunicar-se é o desejado quando pede-se uma pizza através de aplicativos online. Deseja-se um pokémon, objeto virtual que caçamos saíndo de casa porque uma empresa nos disse que ali ele se encontra. Pontos de vista comumente tidos, descrevem tal situação como negativa ou positiva, mas para título deste trabalho vê-se o que precisa ser visto: não é bom nem ruim, apenas é.  Palavras-chave: psicanálise, desejo, Freud, pesquisa, internet, gráficos

MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats

Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy (MALDI-TOF MS) has been used routinely over the past decade in clinical microbiology laboratories to rapidly characterize diverse microorganisms of medical importance both at the genus and species levels. Currently, there is keen interest in applying MALDI-TOF MS at taxonomic levels beyond species and to characterize environmental isolates. We constructed a model system consisting of 19 isolates of Deinococcus aquaticus obtained from biofilm communities indigenous to diverse substrates (concrete, leaf tissue, metal, and wood) in the Fox River – Lake Winnebago system of Wisconsin to: (1) develop rapid sample preparation methods that produce high quality, reproducible MALDI-TOF spectra and (2) compare the performance of MALDI-TOF MS-based profiling to common DNA-based approaches including 16S rRNA sequencing and genomic diversity by BOX-A1R fingerprinting. Our results suggest that MALDI-TOF MS can be used to rapidly and reproducibly characterize environmental isolates of D. aquaticus at the subpopulation level. MALDI-TOF MS provided higher taxonomic resolution than either 16S rRNA gene sequence analysis or BOX-A1R fingerprinting. Spectra contained features that appeared to permit characterization of isolates into two co-occurring subpopulations. However, reliable strain-level performance required rigorous and systematic standardization of culture conditions and sample preparation. Our work suggests that MALDI-TOF MS offers promise as a rapid, reproducible, and high-resolution approach to characterize environmental isolates of members of the genus Deinococcus. Future work will focus upon application of methods described here to additional members of this ecologically diverse and ubiquitous genus

UNDERCOVEREISAGENTEN - ERSTE EXPEDITION UND AKTUELLER STAND DES ARKTISCHEN PERMAFROSTPROJEKTS

Die Menschen in der Arktis sind seit mehreren Jahrzehnten mit Veränderungen ihres Lebensraums, wie dem Auftauen des Permafrosts durch den globalen Klimawandel, konfrontiert. Ziel dieses Projekts ist es, die Erste Expedition und aktueller Stand des arktischen Permafrostprojekts Auswirkungen des Permafrosttauens durch die Erfassung und Analyse von Bildmaterial von UAVs zusammen mit Schüler*innen (SuS) in Kanada und Deutschland zu untersuchen. Während einer Expedition im September 2022 in Nordkanada durch das AWI, DLR und HeiGIT wurden erste UAV-Daten gemeinsam mit SuS der Moose Kerr School in Aklavik aufgenommen. Neben den rund 30000 Einzelfotos über einer Fläche von ca. 13km² wurden die Grundlagen der Datenerhebung sowie die Projektziele der gemeinschaftlichen Permafrost-Untersuchung vermittelt. Vermittelte Ziele sind die selbstständig fortgeführte Datenaufnahme durch interessierte SuS, sowie die selbstständige Formulierung eigener wissenschaftl. Fragestellungen. Es erfolgte plangemäß die Einarbeitung von lokalem Wissen, um weitere Fragestellungen der lokalen Bevölkerung zu adressieren. Die Daten werden aktuell aufbereitet, um über eine Crowdmapping-Anwendung zur Verfügung gestellt zu werden