Unrooted neighbor-joining tree inferred from genetic distances derived from the proportions of alleles shared among 55 Moroccan <i>L. infantum</i> strains based on 14 microsatellites markers.

<p>Two main clusters were detected and the “MON-1” cluster was further sub-divided into two sub-clusters. This was in full agreement with the STRUCTURE results (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077778#pone-0077778-g002" target="_blank">Figure 2</a>). Mixed 1A/1B genotypes were shown in intermediate positions in the tree.</p

Estimated population structure for 33 Moroccan <i>L. infantum</i> strains as inferred by STRUCTURE software on the basis of data for 14 microsatellite markers.

<p>Each of the strains is represented by a single vertical line divided into K colors, where K is the number of populations assumed. Each color represents one population, and the length of the colors segment shows the strain’s estimated proportion of membership in that population.(A) the two main populations derived from the whole dataset which divided strains into MON-1 and non-MON-1 populations. (B) Sub-population analysis of <b>Population 1</b> (MON-1 group) shows two sub-populations. K represents the true number of populations and sub-populations.</p

Map of Morocco with all areas endemic for VL (shadowed areas).

<p>Map of Morocco with all areas endemic for VL (shadowed areas).</p