9 research outputs found
Collagen production of cells cultured on scaffolds.
<p>This was measured by Sirius red assay. Absorbance measured at 490nm per gram of scaffold, n = 3±SEM ***p<0.001.</p
Values for Mechanical properties before and after 7 days of dynamic loading.
<p>Young’s Modulus (<b>top</b>) and ultimate tensile strength (<b>bottom</b>) calculated from stress curves before and after 7 days of uniaxial distension (n-2±SEM), *p<0.05, **p<0.01, ***p<0.001. Dotted lines represent values of healthy paravaginal tissues [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0149971#pone.0149971.ref024" target="_blank">24</a>].</p
Cyclic uniaxial tensile test of the different materials.
<p>(a) Strain-stress plots of the 1<sup>st</sup>, 2<sup>nd</sup> and 5<sup>th</sup> cycle. (b) Plastic deformation (%) at the 2<sup>nd</sup> cycle. (c) Young’s modulus of the 1<sup>st</sup> and 2<sup>nd</sup> cycle (n-3±SEM), *p<0.05, **p<0.01.</p
Stress vs strain plots of the 4 different materials.
<p>Ramp uniaxial tensile test before (A) and after (B) 7 days under dynamic distention in an EBERS bioreactor.</p
Proliferation of cells on scaffolds assessed by assessing cell metabolic activity with AlamarBlue assay (Absorbance measured at 570nm).
<p>(n = 3 ±SEM). *p<0.05 **p<0.01.</p
Fluorescence microscopy and scaffold fibre second harmonic generation using confocal microscopy.
<p>Cells (<b>red</b>) and fibre SHG signal (<b>green</b>) for each of the 5 constructs imaged from the scaffold surface (0μm) to 20μm depth. All scale bars equivalent to 50μm.</p
Scanning electron microscopy images of scaffolds before cell seeding and after 2 weeks of culture with ADSC.
<p>Cells stained with DAPI and Sirius red.</p