Crateriform structures.

<p>(A) Crateriform structures seen from the outside of the cell, indicated by arrows. (B–E) Micrographs of crateriform structures seen from the side, perpendicular to the membrane, indicated by arrows. Outer- (OM), inner-membrane (IM), cytoplasm (C), and periplasm (P) are indicated. Scale bars are 50 nm.</p

Membrane continuity between mother and daughter cells.

<p>Electron micrograph of the neck of the bud. Mother cell (left) and daughter cell (right). Electron dense material is present in the periplasm around the neck. Outer- (OM), inner-membrane (IM), cytoplasm (C) periplasm (P), and electron dense material (EDM) are indicated by arrows. Scale bar is 100 nm.</p

Membrane organization around the neck of the bud.

<p>(A) 3D model displaying membrane organization in proximity to the budding neck. All features are represented and color-coded as in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001565#pbio-1001565-g001" target="_blank">Figure 1</a>. The neck can be observed linking the mother cell (below) to the bud (above). (B) Only the IMs are represented in this image. The more complex organization of the IM can be observed in the mother cell; the single membrane sheet can be observed in the bud. Scale bar is 300 nm.</p

3D reconstruction of bacteria with a complex endomembrane system.

<p>(A) One slice of the tomogram is represented with the different structural features modeled. The OM is in green, the IM in cyan, the DNA in yellow, OM invaginations are in pink, and the Poly-P granule in dark blue. Scale bar is 500 nm. (B) Schematic of the cellular organization of <i>G. obscuriglobus</i>, not to scale. The periplasmic and cytoplasmic spaces are in dark and pale grey, respectively. Other colors are the same as in (A). (C and E) The modeled volume of one cell, sliced roughly through the middle, is represented in different orientations. Two views through the full volume are represented. (D) A slice through the same orientation as in panel (C) might give the false impression of a compartment surrounding the DNA. (F) Full volume representation without the membranes showing the five fragments of DNA, the crateriform structures, and the granule.</p