DataSheet1.pdf

<p>We aimed to identify and quantify CD117⁺ and CD90⁺ endogenous cardiac progenitor cells (CPC) in human healthy and diseased hearts. We hypothesize that these cells perform a locally acting, contributing function in overcoming medical conditions of the heart by endogenous means. Human myocardium biopsies were obtained from 23 patients with the following diagnoses: Dilatative cardiomyopathy (DCM), ischemic cardiomyopathy (ICM), myocarditis, and controls from healthy cardiac patients. High-resolution scanning microscopy of the whole slide enabled a computer-based immunohistochemical quantification of CD117 and CD90. Those signals were evaluated by Definiens Tissue Phenomics® Technology. Co-localization of CD117 and CD90 was determined by analyzing comparable serial sections. CD117⁺/CD90⁺ cardiac cells were detected in all biopsies. The highest expression of CD90 was revealed in the myocarditis group. CD117 was significantly higher in all patient groups, compared to healthy specimens (^*p < 0.05). The highest co-expression was found in the myocarditis group (6.75 ± 3.25 CD90⁺CD117⁺ cells/mm²) followed by ICM (4 ± 1.89 cells/mm²), DCM (1.67 ± 0.58 cells/mm²), and healthy specimens (1 ± 0.43 cells/mm²). We conclude that the human heart comprises a fraction of local CD117⁺ and CD90⁺ cells. We hypothesize that these cells are part of local endogenous progenitor cells due to the co-expression of CD90 and CD117. With novel digital image analysis technologies, a quantification of the CD117 and CD90 signals is available. Our experiments reveal an increase of CD117 and CD90 in patients with myocarditis.</p