Characteristics of the ESBL/pAmpC-producing isolates.

<p>^a The first four numbers of the isolate name indicate the person sampled, the number after the underscore indicates the isolate number.</p><p>^b The CTX-M-14 has four synonymous mutations (A372G, G570A, G702A, A875G) in comparison to the official entry for this allele (accession number AF252622).</p><p>^c The TEM-52 gene has one synonymous mutation (C228T) in comparison to the official entry for this TEM allele (accession number Y13612).</p><p>CC = clonal complex; nd = not determined; NTP = non-typeable incompatibility group plasmid.</p><p>Characteristics of the ESBL/pAmpC-producing isolates.</p

Primers used to completely sequence the ESBL/AmpC resistance genes.

<p>Primers used to completely sequence the ESBL/AmpC resistance genes.</p

IgA levels.

<p>IgA responses specific for PT (A), FHA (B) and Prn (C) for each child in the on the <i>x</i>-axis presented group of children. The red horizontal bars indicate the GMC. The 10 and 28 days post-booster responses are indicated by the black circles and all other groups are indicate by open circles. * significant differences (p<0.05) are indicated for the healthy children between the presented age groups in similar primed children. Differences between infected children and the various groups of healthy wP and aP primed children are described in paragraph 3.1 of the results section.</p

The effect of the time of blood sampling since the start of pertussis symptoms on serum IgA responses to PT, FHA and Prn in children younger and older than 4 years of age.

<p>*significantly higher as compared to >60 days GMC.</p><p>∫significantly higher as compared to <30 days GMC.</p>#<p>significantly higher as compared to GMC at same time point in children <4 years of age.</p

Correlations between serum IgA and IgG responses.

<p>Spearman correlations and linear regression analysis between serum IgA and serum IgG responses specific for PT, FHA and Prn presented for infected (A), wP (B) and aP (C) primed children.</p

The number of healthy children per age group.

<p>(A) children primed with the Dutch wP vaccine and (B) children primed with aP vaccines.</p

Baseline characteristics of surgeries with bundles not reported, partially reported, entirely reported but not fully compliant, and entirely reported with full compliance.

<p>Baseline characteristics of surgeries with bundles not reported, partially reported, entirely reported but not fully compliant, and entirely reported with full compliance.</p

The percentage of non-susceptibility (NS) for the most commonly isolated uropathogens separately, and the probability of inadequate coverage (e.g., weighted average) for uropathogens combined, ISIS-AR, the Netherlands 2012.

<p>IR =  intrinsic resistant, NA = not available, LLR = low level resistance.</p>?<p> We only included resistance to meropenem for this bacterium.</p>#<p> For Enterobacteriaceae the breakpoint for <i>E. coli</i> was used.</p>*<p> The distribution of species (e.g., number of isolates of each species) was taken into account when calculating the probability of inadequate coverage (e.g., weighted average); the overall resistance percentages are therefore the resistance percentages that would be encountered when choosing empirical treatment.</p><p>AMX: amoxicillin, AMC: amoxicillin-clavulanic acid, CXM: cefuroxime, CAZ: ceftazidime, CTX: cefotaxime, CRO: ceftriaxone, 3GC: 3rd generation cephalosporins, CARB: carbapenems, CIP: ciprofloxacin, SXT: trimethoprim-sulphamethoxazole, NIT: nitrofurantoin, GEN: gentamicin.</p

Reporting of and compliance with the bundle and its elements.

<p>Reporting of and compliance with the bundle and its elements.</p

Pathogen distribution of various urine sample types and patient groups, ISIS-AR, the Netherlands, 2012.

<p>*We considered an infection to represent urosepsis when a blood specimen was submitted from the same patient, with the same pathogen within 7 days of a urinary specimen with that pathogen **We considered a UTI community onset if the urine sample was collected within two days after hospital admission *** We considered a UTI hospital associated if the urine sample was collected after the second day of hospital admission.</p