One thousand DNA barcodes of piranhas and pacus reveal geographic structure and unrecognised diversity in the Amazon

Piranhas and pacus (Characiformes: Serrasalmidae) are a charismatic but understudied family of Neotropical fishes. Here, we analyse a DNA barcode dataset comprising 1,122 specimens, 69 species, 16 genera, 208 localities, and 34 major river drainages in order to make an inventory of diversity and to highlight taxa and biogeographic areas worthy of further sampling effort and conservation protection. Using four methods of species discovery - incorporating both tree and distance based techniques - we report between 76 and 99 species-like clusters, i.e. between 20% and 33% of a priori identified taxonomic species were represented by more than one mtDNA lineage. There was a high degree of congruence between clusters, with 60% supported by three or four methods. Pacus of the genus Myloplus exhibited the most intraspecific variation, with six of the 13 species sampled found to have multiple lineages. Conversely, piranhas of the Serrasalmus rhombeus group proved difficult to delimit with these methods due to genetic similarity and polyphyly. Overall, our results recognise substantially underestimated diversity in the serrasalmids, and emphasise the Guiana and Brazilian Shield rivers as biogeographically important areas with multiple cases of across-shield and within-shield diversifications. We additionally highlight the distinctiveness and complex phylogeographic history of rheophilic taxa in particular, and suggest multiple colonisations of these habitats by different serrasalmid lineages. © 2018 The Author(s)

Baited remote underwater video as a promising nondestructive tool to assess fish assemblages in clearwater Amazonian rivers: Testing the effect of bait and habitat type

Baited remote underwater video (BRUV) systems are being used in marine ecosystems as a nonextractive, cost-effective method of assessing the fish fauna with minimal species bias. This technique has had limited applications in freshwater ecosystems. Rheophilic fish assemblages of the Xingu River, a clearwater Amazonian river in Northern Brazil, were sampled with BRUV systems. Two-hour video recordings were collected using five different bait treatments (sardine, croaker, cat food, sweet corn, and no bait) in two lotic habitat categories (rocky and sandy bottoms). A total of 2460 fish from 56 taxa and 13 families were recorded from the 80 BRUV deployments. Significantly different fish assemblages, species richness, and abundance were detected between habitat types and among treatments. Our results suggest that the use of crushed sardines as a standardized bait optimizes the sampling recording the highest species richness, relative abundance, and number of exclusive species of rheophilic fish in clearwater Amazonian rivers. The data also highlight the unique fish diversity of the Xingu River prior to the expected large-scale environmental degradation resulting from the forthcoming operation of the Belo Monte hydroelectric power plant