High-Value Components and Bioactives from Sea Cucumbers for Functional Foods—A Review

Sea cucumbers, belonging to the class Holothuroidea, are marine invertebrates, habitually found in the benthic areas and deep seas across the world. They have high commercial value coupled with increasing global production and trade. Sea cucumbers, informally named as bêche-de-mer, or gamat, have long been used for food and folk medicine in the communities of Asia and Middle East. Nutritionally, sea cucumbers have an impressive profile of valuable nutrients such as Vitamin A, Vitamin B1 (thiamine), Vitamin B2 (riboflavin), Vitamin B3 (niacin), and minerals, especially calcium, magnesium, iron and zinc. A number of unique biological and pharmacological activities including anti-angiogenic, anticancer, anticoagulant, anti-hypertension, anti-inflammatory, antimicrobial, antioxidant, antithrombotic, antitumor and wound healing have been ascribed to various species of sea cucumbers. Therapeutic properties and medicinal benefits of sea cucumbers can be linked to the presence of a wide array of bioactives especially triterpene glycosides (saponins), chondroitin sulfates, glycosaminoglycan (GAGs), sulfated polysaccharides, sterols (glycosides and sulfates), phenolics, cerberosides, lectins, peptides, glycoprotein, glycosphingolipids and essential fatty acids. This review is mainly designed to cover the high-value components and bioactives as well as the multiple biological and therapeutic properties of sea cucumbers with regard to exploring their potential uses for functional foods and nutraceuticals

Improvement of glucose production by raw starch degrading enzyme utilizing acid-treated sago starch as substrate

The native sago starch exists as a compact crystalline structure and is not efficiently hydrolyzed by Raw Starch Degrading Enzyme (RSDE). In order to enhance its hydrolysability, the starch was treated with acid and heated below its gelatinization temperature, thus increasing the accessibility of the sago starch granule to enzymatic attack. Results showed that treatment of sago starch with acid at pH 2.0 and temperature 65oC for 2 hours greatly enhanced its conversion rate to glucose from 53.3% to 71.9%. It is clearly shown that high yield of glucose is produced during hydrolysis of acid-treated sago starch using the Raw Starch Degrading Enzyme from Acremonium sp. The difference between the acid-treated and untreated sago starch in this study could be due to the differences on the surface of the sago starch granule which may influence the accessibility and diffusion of enzyme into the starch during hydrolysis

Characterisation of the ability of globulins from legume seeds to produce cocoa specific aroma

This study was carried out to extract and compare the characteristic ability of globulins from cottonseed, alfalfa seed, pea seed, mung bean and French bean with cocoa seeds to produce cocoa-specific aroma precursors. The extracted globulins were compared through SDS PAGE, amino acid and oligopeptide profiles. A very low recovery was obtained during globulin extraction from different seeds ranging from 0.5% to 2.7%. Cottonseed produced the highest total protein (13.90 mg/g), followed by cocoa seed (11.91 mg/g), whereas alfalfa seed, mung bean, pea seed and French bean produced 7.86, 4.77, 4.59 and 3.89 mg/g respectively. Two distinctive bands of 51.1 and 33.0 kDa were observed for cocoa vicilin-class globulin (VCG) from SDS PAGE. More than three bands were shown for other seed globulins. Comparative HPLC analyses of the obtained peptide mixtures revealed different and complex patterns of predominantly hydrophobic peptides. A similar high content of amides (glutamic acids-glutamine, aspartic acid- asparagine and arginine) and low concentrations of lysine were observed in all seeds globulin

Functional and pasting properties of a tropical breadfruit (Artocarpus altilis) starch from Ile-Ife, Osun State, Nigeria

This study was carried out to determine the proximate, functional and pasting properties of breadfruit starch. Breadfruit starch was isolated from matured breadfruit (Artocarpus altilis) and was analyzed for its functional, proximate and pasting properties. The starch contains 10.83%, 0.53%, 0.39%, 22.52%, 77.48% and 1.77% moisture, crude protein, fat, amylose, amylopectin and ash contents respectively. The average particle size, pH, bulk density and dispersibility of the breadfruit starch were 18 μm, 6.5, 0.673 g/mls, and 40.67% respectively. The swelling power of the breadfruit starch increases with increase in temperature, but there was a rapid increase in the swelling power from 70 to 80°C. The pasting temperature of the starch paste was 84.05°C, setback and breakdown values were 40.08 and 7.92 RVU respectively. The peak viscosity value was 121.25 RVU while final viscosity value was 153.42 RVU. This study concluded that breadfruit starch has an array of functional, pasting and proximate properties that can facilitate its use in so many areas where the properties of other starches are acceptable

Inter-varietal variation in the composition of seeds and seed oils from winter mel on [Benincasa Hispida (thunb.) cogn.] fruits.

Winter melon (Benincasa hispida), locally known as Kundur, is a vegetable crop, popular, especially among Asian communities both for nutritional and medicinal attributes. In the present work, physicochemical properties of seeds and the extracted seed oils were examined and compared among three cultivars namely round, oval and hybrid of winter melon. The seeds from round, oval and hybrid fruits, exhibited protein, fiber and ash contents 28.18-42.03, 19.36-26.21 and 5.02- 11.81%, respectively. The oils were extracted based on Soxhlet method by petroleum ether, whilst yields ranged from 17.78- 32.53% (wt/wt). The extracted oils were analyzed for physicochemical parameters, and fatty acids, tocopherols and sterols profiles. The results for specific gravity (25°C), refractive index (nD 25°C), iodine value (IV), saponification value (SV), peroxide value (PV), and free fatty acid (% as oleic acid) were 0.89-0.91 g/mL, 1.4627-1.4646, 119.9-125.1 g I/100 g oil, 182.3-194.1 mg KOH/ g oil, 1.13-1.33 mequiv.O2 /kg, 1.57-2.10%, respectively. The oil color intensity in terms of yellow and red units was 6.9Y + 1.0 R to 8.9Y + 1.9R. The amounts of oil tocopherols as analyzed by HPLC varied widely among the cultivars tested showing α-tocopherol 31.1-207.6 mg/kg and δ-tocopherol 60.4-146.0 mg/kg. According to the GLC analysis linoleic acid (C18:2) was established to be the principal fatty acid (63.10-70.64%) followed by C16:0 (12.45-17.59), C18:1 (8.46-12.87%) and C18:0 (5.13-7.48%). Analysis of oil sterol fractions, using GC and GC-MS, revealed the presence of β-sitosterol (54.62–60.50%), campesterol (15.10–18.50%), stigmasterol (11.00–14.30% and Δ 5 -avenasterol (6.40–8.14 %) as the four main components. Most of the properties of the seed oils analyzed varied significantly among fruit cultivars tested. Overall, we concluded that the seeds, which are under-utilized and often discarded as an agrowate, from winter melon should be explored for extraction of high-linoleic oil with additional tocopherols and phytosterol benefits

Antioxidative activities of chromatographic fractions obtained from root, fruit and leaf of Mengkudu (Morinda citrifolia L.)

Crude extracts of root, leaf and fruit of Morinda citrifolia were fractionated on a Sephadex LH-20 column with ethanol as eluate. Based on UV absorption intensity of phenolic compound (725 mm) the Sephadex LH-20 column was able to separate fruit, leaf and root extracts into six, five and five fractions, respectively. The results showed that all the fractions tested exhibited considerably high antioxidative activity in the ferric thiocyanate assay and thiobarbituric acid test and the activities of some of the fractions were as good as those of either tocopherol or BHT. The fractions from different parts of the plants were found to contain different amounts of total phenolic compounds, which, interestingly, do not correspond to the antioxidative activity measured. This is probably due to the presence of different phenolics in the samples, with different antioxidative activities which involves various mechanisms inhibiting the oxidation process. The study suggested that root, leaf and fruit of M. citrifolia might contribute significantly to exogenous antioxidant which is crucial in combating oxidative stress