TGF-β1 increases SERT levels on the luminal surface in 3D-culture of Caco-2 cells.

<p>Caco-2 cells in 3D culture system untreated or treated with TGF-β1 (10 ng/ml, 1h) were stained for SERT (green), Phalloidin (red) and DAPI (blue) and visualized by confocal microscopy. XY planar images and orthogonal XZ images Orthognal <i>xz</i> images were obtained with a Zeiss LSM 510 confocal microscope.</p

SERT function in response to TGF-β1 treatment of native mouse ileum.

<p>The distal small intestine was stripped off of the muscle layer, mounted in the Ussing chamber to expose tissue on both sides to Kreb’s solution supplemented with L-ascorbic acid (100 μM) and the monoamine oxidase inhibitor pargyline (100 μM). Tissues were treated with TGF-β1 for 1h from basolateral side and then incubated with ³[H]-5-HT for 30 min. Fluoxetine (10 μM) was added 30 min prior to TGF-β1 treatment from the apical side. Values represent mean ± SEM of 3 different experiments. *<i>P</i><0.05 compared with untreated control.</p