Effectiveness of an integrated adventure-based training and health education program in promoting regular physical activity among childhood cancer survivors

Background: There is growing concern about declining levels of physical activity in childhood cancer survivors. This study aimed to examine the effectiveness of an integrated adventure-based training and health education program in promoting changes in exercise behavior and enhancing the physical activity levels, self-efficacy, and quality of life of Hong Kong Chinese childhood cancer survivors. Methods: A randomized controlled trial, two-group pretest and repeated post-test, between-subjects design was conducted to 71 childhood cancer survivors (9- to 16-year-olds). Participants in the experimental group joined a 4-day integrated adventure-based training and health education program. Control group participants received the same amount of time and attention as the experimental group but not in such a way as to have any specific effect on the outcome measures. Participants' exercise behavior changes, levels of physical activity, self-efficacy, and quality of life were assessed at the time of recruitment, 3, 6, and 9 months after starting the intervention. Results: Participants in the experimental group reported statistically significant differences in physical activity stages of change (p < 0.001), higher levels of physical activity (p < 0.001) and self-efficacy (p = 0.04) than those in the control group. Besides, there were statistically significant mean differences (p < 0.001) in physical activity levels (-2.6), self-efficacy (-2.0), and quality of life (-4.3) of participants in the experimental group from baseline to 9 months after starting the intervention. Conclusions: The integrated adventure-based training and health education program was found to be effective in promoting regular physical activity among childhood cancer survivors. Copyright © 2013 John Wiley & Sons, Ltd.postprin

How and why DNA barcodes underestimate the diversity of microbial eukaryotes

Background: Because many picoplanktonic eukaryotic species cannot currently be maintained in culture, direct sequencing of PCR-amplified 18S ribosomal gene DNA fragments from filtered sea-water has been successfully used to investigate the astounding diversity of these organisms. The recognition of many novel planktonic organisms is thus based solely on their 18S rDNA sequence. However, a species delimited by its 18S rDNA sequence might contain many cryptic species, which are highly differentiated in their protein coding sequences. Principal Findings: Here, we investigate the issue of species identification from one gene to the whole genome sequence. Using 52 whole genome DNA sequences, we estimated the global genetic divergence in protein coding genes between organisms from different lineages and compared this to their ribosomal gene sequence divergences. We show that this relationship between proteome divergence and 18S divergence is lineage dependant. Unicellular lineages have especially low 18S divergences relative to their protein sequence divergences, suggesting that 18S ribosomal genes are too conservative to assess planktonic eukaryotic diversity. We provide an explanation for this lineage dependency, which suggests that most species with large effective population sizes will show far less divergence in 18S than protein coding sequences. Conclusions: There is therefore a trade-off between using genes that are easy to amplify in all species, but which by their nature are highly conserved and underestimate the true number of species, and using genes that give a better description of the number of species, but which are more difficult to amplify. We have shown that this trade-off differs between unicellular and multicellular organisms as a likely consequence of differences in effective population sizes. We anticipate that biodiversity of microbial eukaryotic species is underestimated and that numerous ''cryptic species'' will become discernable with the future acquisition of genomic and metagenomic sequences

Epidemiology of Japanese Encephalitis in the Philippines prior to routine immunization.

BACKGROUND In 2015, we published our findings highlighting the endemicity of Japanese encephalitis (JE) in the Philippines. The policymakers responded by conducting an immunization campaign and strengthening the surveillance system. Using the data on the revitalized surveillance system, we updated the epidemiology of JE in the country METHODS: Electronic databases were searched, and conference proceedings related to JE in the Philippines were identified until 31 December 2018. We used surveillance data from 1 January 2014 to 31 December 2017. We used the 2015 population census to estimate the national and regional incidence for children <15 years old. RESULTS Four studies reported on the seroprevalence of JE in the Philippines, which showed increasing seroprevalence with increasing age. Seroprevalence rates were from zero for infants (<1 year old) to 65.7% in adolescents (12-18 years old) before the immunization campaign. Among five studies on clinical profile JE, case fatality ranged from zero to 21.1%, and neurologic sequelae ranged from 5.2% to 81.8% of diagnosed cases. In the surveillance data, JE cases peaked annually from July to October, coinciding with the wet season. The national incidence was estimated at 0.7 JE cases/100,000 among children <15 years at a minimum, but higher rates were seen in the northern regions of the country. CONCLUSION Improved surveillance affirmed the burden of JE in the Philippines. A subnational immunization campaign in April 2019 was conducted in the northern regions of the country. We highlight the importance of the inclusion of JE vaccine in the immunization program and sustained high-quality surveillance to monitor its impact on JE control

Using the MitoB method to assess levels of reactive oxygen species in ecological studies of oxidative stress

In recent years evolutionary ecologists have become increasingly interested in the effects of reactive oxygen species (ROS) on the life-histories of animals. ROS levels have mostly been inferred indirectly due to the limitations of estimating ROS from in vitro methods. However, measuring ROS (hydrogen peroxide, H2O2) content in vivo is now possible using the MitoB probe. Here, we extend and refine the MitoB method to make it suitable for ecological studies of oxidative stress using the brown trout Salmo trutta as model. The MitoB method allows an evaluation of H2O2 levels in living organisms over a timescale from hours to days. The method is flexible with regard to the duration of exposure and initial concentration of the MitoB probe, and there is no transfer of the MitoB probe between fish. H2O2 levels were consistent across subsamples of the same liver but differed between muscle subsamples and between tissues of the same animal. The MitoB method provides a convenient method for measuring ROS levels in living animals over a significant period of time. Given its wide range of possible applications, it opens the opportunity to study the role of ROS in mediating life history trade-offs in ecological settings

Intracellular interferons in fish : a unique means to combat viral infection

Peer reviewedPublisher PD

On stable higher spin states in Heterotic String Theories

We study properties of 1/2 BPS Higher Spin states in heterotic compactifications with extended supersymmetry. We also analyze non BPS Higher Spin states and give explicit expressions for physical vertex operators of the first two massive levels. We then study on-shell tri-linear couplings of these Higher Spin states and confirm that BPS states with arbitrary spin cannot decay into lower spin states in perturbation theory. Finally, we consider scattering of vector bosons off higher spin BPS states and extract form factors and polarization effects in various limits.Comment: 38 page

The small GTPase Rab29 is a common regulator of immune synapse assembly and ciliogenesis

Acknowledgements We wish to thank Jorge Galán, Gregory Pazour, Derek Toomre, Giuliano Callaini, Joel Rosenbaum, Alessandra Boletta and Francesco Blasi for generously providing reagents and for productive discussions, and Sonia Grassini for technical assistance. The work was carried out with the financial support of Telethon (GGP11021) and AIRC.Peer reviewedPostprin

Pair Production of small Black Holes in Heterotic String Theories

We study pair production of small BPS BH's in heterotic strings compactified on tori and in the FHSV model. After recalling the identification of small BH's in the perturbative BPS spectrum, we compute the tree-level amplitudes for processes initiated by massless vector bosons or gravitons. We then analyze the resulting cross sections in terms of energy and angular distributions. Finally, we briefly comment on scenari with large extra dimensions and on generalizations of our results to non-BPS, non-extremal and rotating BH's.Comment: 33 page