A Lopez-Munoz

A Matsuo

A Pindel

AJ Sadler

B Sun

Bei Huang

Bertrand Collet

C Stein

Chris J. Secombes

CM Ahmed

D Aggad

E Kalie

EL Ooi

F Sun

FF Yu

J Zou

JM Gonzalez-Navajas

Jun Zou

Karen L. Mossman

KE Taylor

M Chang

M Shin-Ya

Ming-Xian Chang

MK Purcell

N Sathish

O Haller

OJ Hamming

PE Phelan

Pin Nie

S Biacchesi

S Campbell

S Long

SA Martin

SV Kotenko

SY Liu

T Svingerud

V Bergan

Y Palti

YB Zhang

Z Li

Z Qi

Zhanglong Yu

PLoS Pathogens

English

PubMed

We demonstrate for the first time in vertebrates, that alternative splicing of interferon (IFN) genes can lead to a functional intracellular IFN (iIFN). Fish IFN genes possess introns and in rainbow trout three alternatively spliced transcripts of the IFN1 gene exist. Two of the encoded IFNs are predicted to lack a signal peptide. When overexpressed these iIFNs induce antiviral responses. Variants of the two IFNR receptor chains (IFNAR1 and IFNAR2) lacking a signal peptide are also present in trout. Transfection of HEK 293T cells with the iIFN and iIFNR molecules results in STAT phosphorylation and induction of antiviral genes. These results show that fish possess a functioning iIFN system that may act as a novel defence to combat viral infection

Ming-xian Chang

CiteSeerX

Intracellular Interferons in Fish: A Unique Means to Combat Viral Infection

Chris J Secombes

Directory of Open Access Journals

Intracellular interferons in fish: a unique means to combat viral infection.

Chang, Ming-Xian

Zou, Jun

Nie, Pin

Huang, Bei

Yu, Zhanglong

Collet, Bertrand

Secombes, Chris J

Aberdeen University Research

Administration of recombinant IFN1 protects zebrafish (Danio rerio) from ISKNV infection.

Atlantic salmon type I subtypes show differences in antiviral activity and cell-dependent expression: Evidence for high IFNb/IFNc-producing cells in fish lymphoid tissues.

Biological characterisation of a recombinant Atlantic salmon type I interferon synthesized in Escherichia coli.

Characterization of the interferon genes in homozygous rainbow trout reveals two novel genes, alternate splicing and differential regulation of duplicated genes.

Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish.

Crystal structure of zebrafish interferons I and II reveals conservation of type I interferon structure in vertebrates.

Evasion and subversion of interferon-mediated antiviral immunity by Kaposi’s Sarcoma-associated Herpesvirus: An overview.

Fish MITA serves as a mediator for distinct fish IFN gene activation dependent on IRF3 or IRF7.

Fish virus-induced interferon exerts antiviral function through Stat1 pathway.

Functional characterization of fulllength TLR3, IRAK-4, and TRAF6 in zebrafish (Danio rerio).

Identification and bioactivities of IFN-gamma in rainbow trout Oncorhynchus mykiss: the first Th1-type cytokine characterized functionally in fish.

Identification and expression analysis of cDNAs encoding channel catfish type I interferons.

Identification of a cDNA encoding channel catfish interferon.

Identification of a second group of type I IFNs sheds light on IFN evolution in vertebrates.

Identification of an additional two-cysteine containing type I interferon in rainbow trout Oncorhynchus mykiss provides evidence of a major gene duplication event within this gene family in teleosts.

Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties.

Identification, characterization and genetic mapping of TLR7, TLR8a1 and TLR8a2 genes in rainbow trout (Oncorhynchus mykiss).

IFN-lambdas.

Immunomodulatory functions of type I interferons.

Interferon response following infection with genetically similar isolates of viral haemorrhagic septicaemia virus (VHSV) exhibiting contrasting virulence in rainbow trout.

Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray.

Interferon-inducible antiviral effectors.

Intracellular interferon triggers Jak/Stat signaling cascade and induces p53-dependent antiviral protection.

Intron-containing type I and III IFN coexist in amphibians: refuting the concept that a retroposition event gave rise to type I IFNs.

Mitochondrial antiviral signaling protein plays a major role in induction of the fish innate immune response against RNA and DNA viruses.

Molecular regulation of interferon antiviral response in fish.

New developments in the induction and antiviral effectors of type I interferon.

New insights into the evolution of IFNs: zebrafish group II IFNs induce a rapid and transient expression of IFN-dependent genes and display powerful antiviral activities.

Origin and evolution of the RIG-I like RNA helicase gene family.

Promoters of type I interferon genes from Atlantic salmon contain two main regulatory regions.

Recent advances in understanding viral evasion of type I interferon.

Sadler A

Selective expression of nonsecreted interferon by an adenoviral vector confers antiproliferative and antiviral properties and causes reduction of tumor growth in nude mice.

Teleost fish interferons and their role in immunity.

Teleost TLR22 recognizes RNA duplex to induce IFN and protect cells from birnaviruses.

The stability of the ternary interferon-receptor complex rather than the affinity of the individual subunits dictates different biological activities.

The two groups of zebrafish virus-induced interferons signal via distinct receptors with specific and shared chains.

Intracellular interferons in fish : a unique means to combat viral infection

<div><p>We demonstrate for the first time in vertebrates, that alternative splicing of interferon (IFN) genes can lead to a functional intracellular IFN (iIFN). Fish IFN genes possess introns and in rainbow trout three alternatively spliced transcripts of the IFN1 gene exist. Two of the encoded IFNs are predicted to lack a signal peptide. When overexpressed these iIFNs induce antiviral responses. Variants of the two IFNR receptor chains (IFNAR1 and IFNAR2) lacking a signal peptide are also present in trout. Transfection of HEK 293T cells with the iIFN and iIFNR molecules results in STAT phosphorylation and induction of antiviral genes. These results show that fish possess a functioning iIFN system that may act as a novel defence to combat viral infection.</p></div

Intracellular interferons in fish : a unique means to combat viral infection

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