Polydopamine coated capillaries for CE separations

International audienc

Technologies PNIPAM pour les laboratoires sur puce

TOULOUSE3-SCD-Bib. electronique (315559904) / SudocSudocFranceF

An easy surface modification in heterogeneous nano- and micro- fluidic devices

International audienc

A new and easy surface functionalization technnology for monitoring wettability in heterogeneous nano- and microfluidic devices

International audienceWe have developed a new and easy surface functionalization technology for monitoring wettability in heterogeneous nano- and microfluidic devices. This technology is based on mussel inspired, dopamine chemistry and it permits to end-graft hydrophilic polymer brushes onto virtually any surface in a low-viscosity regime. We have successfully modified a variety of different solid surfaces, such as Si, SiO2, Ag, Cu, SU8 and PDMS. The modified surfaces were characterized by water contact angle measurements, atomic force microscopy, cyclic voltammetry and by electrokinetic measurements. The adsorption of proteins on the unmodified and modified surfaces was probed with fluorescently labeled albumin. We clearly demonstrated that the studied surfaces became non-fouling when modified with a hydrophilic, polyacrylamide brush, while they remained protein adsorbing when unmodified and/or modified with the dopamine film only. We believe that this universal surface modification approach will be extremely useful in nano- and micro-fluidic devices build from a variety of different materials

Système microfluidique et procédé pour isoler et quantifier au moins une sous-population de cellules à partir d'une population de cellules

The invention concerns a microfluidic System (1) capable of receiving cell populations and capable of simultaneously isolating and quantifying, for each of the populations, at least one sub-population of cells {ÇA), and a method of simultaneous isolation and quantification using this System. The System comprises a substrate having networks of microchannels (10) comprising a first sorting unit capable of isolating, by magnetic attraction, cells of interest (C2) in the population in at least one first sorting micro-channel. This network comprises a second unit for simultaneous sorting and counting (U2) comprising at least one second sorting microchannel (10) defined by a closed wall (S) having an inner face (9) provided with at least one functionalised électrode (El,E2) capable of trapping a sub-population, the second unit comprising means (El,E2) for counting the sub-population by means of impedance spectroscopy. According to the invention, the second sorting microchannel is provided with at least one pair of opposing functionalised électrodes and at least one pair of second micro-coils (11and12) for trapping the cells of interest arranged in the wall facing the électrodes and controlling successive attraction/release cycles that alternate between the micro-coils.L'invention concerne un système microfiuidique (1) apte à recevoir des populations de cellules et apte à isoler et à quantifier simultanément pour chacune des populations au moins une sous-population de cellules {ÇA), et un procédé d'isolement et de quantification simultanés utilisant ce système. Le système comporte un substrat à réseau de microcanaux (10) comprenant, une première unité de tri apte à isoler par attraction magnétique des cellules d'intérêt (C2) de la population dans au moins un microcanal de premier tri. Ce réseau comprend une seconde unité de tri et de comptage simultanés (U2) comportant au moins un microcanal de second tri (10) défini par une paroi fermée (S) à face interne (9) pour vue d'au moins une électrode fonctionnalisée (El,E2) apte à piéger une sous-population, la seconde unité comportant des moyens de comptage (El,E2) par spectroscopie d'impédance de la sous-population. Selon l'invention, te micfocanal de second tri est' pourvu d'au moins une paire d'électrodes fonctionnalisées opposées et d'au moins une paire de secondes micro-bobines de piégeage (11et12) des cellules d'intérêt agencées dans la paroi en regard des électrodes et commandant des cycles d'attraction/libération successifs et alternés entre lesmicro-bobines

Synthesis of 6-Amino-5-cyano-1,4-disubstituted-2(1H)-Pyrimidinones via Copper-(I)-catalyzed Alkyne-azide ‘Click Chemistry’ and Their Reactivity

In this paper we present the room temperature synthesis of a novel serie of 1,4-disubstituted-1,2,3-triazoles 4a-l by employing the (3 + 2) cycloaddition reaction of pyrimidinones containing alkyne functions with different model azides in the presence of copper sulphate and sodium ascorbate. To obtain the final triazoles, we also synthesized the major precursors 6-amino-5-cyano-1,4-disubstituted-2(1H)-pyrimidinones 3a-r from ethyl 2,2-dicyanovinylcarbamate derivatives 2a-c and various primary aromatic amines containing an alkyne group. The triazoles were prepared in good to very good yields

CLICK CHEMISTRY AS AN EFFICIENT TOOL TO ACCESS 6-AMINO-5-CYANO-2(1H)-PYRIMIDINONE DIMERS

Abstract We describe here the synthesis of triazole-linked 6-amino-5-cyano-2(1H)-pyrimidinone dimers using 1,3-dipolar cycloaddition. The azido-precursor was prepared through the reaction of ethyl 2,2-dicyanovinylcarbamate derivatives with p-azidoaniline. The second precursor, a 6-amino-5-cyano-1-(3 or 4-ethynylphenyl)-4-substituted-2(1H)-pyrimidinone, was obtained from ethyl 2,2-dicyanovinylcarbamate derivatives reacting with 3-ethynylaniline or 4-ethynylaniline. Consequently, the two 'click systems' were mixed together in order to obtain the desired dimer