Linoleic and Linolenic Acids Analysis of Soybean Tofu with Rhizopus Oryzae and Rhizopus Oligosporus as Coagulant

SudaryatiningsihC, Supyani. 2009. Linoleic and linolenic acids analysis of soybean tofu with Rhizopus oryzae and Rhizopusoligosporus as coagulant. Nusantara Bioscience 1: 110-116. The aims of this research are to know the potency of Rhizopus oligosporusand Rhizopus oryzae as a coagulant in tofu processing for increasing the amount of linoleic and linolenic acids, and to know the timethat needed by R. oligosporus and R. oryzae for increasing the amount of linoleic and linolenic acids. It uses PDA for inoculating fungi,and it is done at Sub-Lab Chemistry, Central Laboratory for Mathematics and Natural Sciences, Sebelas Maret University, Surakarta.The tofu making was done in “Dele Emas†Tofu Factory, Surakarta. Analysis of linoleic and linolenic acids were done by GasChromatography, in LPPT-UGM Yogyakarta. The conclusion of this research are R. oligosporus dan R. oryzae having a potency as acoagulant in tofu processing for increasing the amount of linoleic and linolenic acids. R. oryzae needs 18 hours to coagulate the tofu, andR. oligosporus needs 12 hours for the same process. The highest amount of linoleic and linolenic acids were obtained by R. oryzae at 6hours of fermentation (0.26% and 0.14%), and 24 hours of fermentation by R. oligosporus (0.06% and 0.04%)