Muscle malate dehydrogenase activity of Astyanax bimaculatus from una river basin as a biomarker of environmental impact [Atividade da malato desidrogenase muscular de Astyanax bimaculatus da bacia hidrográfica do rio una como biomarcadora de impacto ambiental]

Made available in DSpace on 2019-09-12T16:26:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2016The enzymatic activity of malate dehydrogenase (MDH) and its isoforms may vary in fishes according their living environment. The aim of this work was to investigate the activity of MDH in the muscle tissue of Astyanax bimaculatus (locally called as lambari do rabo amarelo), collected at three different points (P1, P2 and P3) in the Una River Basin (Taubaté, SP), to evaluate it as a candidate biomarker for environmental change. Cytosolic and mitochondrial isoforms of MDH were extracted from muscles. The results indicated that the total enzyme activity was 1.8 and 2.5 times higher in A. bimaculaus at P2, in comparison to specimens collected at P1 and P3, respectively. Seven MDH isoforms were detected in the muscle tissue (MDH-1 to MDH-7). MDH-1 levels of specimens collected at P3 were 1.9 and 2.4 times higher than those from P1 and P2, respectively. However, the levels of MDH-5,-6 and-7 of P3 specimens were significantly lower than P1 ones. These results are possibly related to water quality, considering that P3 is the most polluted among the three sampling sites. The muscle MDH activity of A. bimaculatus was modulated by the environment. Therefore, it can be concluded that this enzyme could be used as a biomarker of environmental change of the hydrographic basin of a neotropical region, where this species occurs. © 2016, Institute for Environmental Research in Hydrographic Basins (IPABHi). All rights reserved.de Almeida, M.E., Instituto Básico de Biociências, Universidade de Taubaté (UNITAU), Taubaté, SP, BrazilBatista, M.T.O., Instituto Básico de Biociências, Universidade de Taubaté (UNITAU), Taubaté, SP, Brazilde Oliveira, M.F., Universidade Federal do Paraná (UFPR), Curitiba, PR, BrazilVani, G.S., Instituto Básico de Biociências, Universidade de Taubaté (UNITAU), Taubaté, SP, Brazil, Universidade de Taubaté (UNITAU), Taubaté, SP, BrazilRodrigues, E., Instituto Básico de Biociências, Universidade de Taubaté (UNITAU), Taubaté, SP, Brazil, Universidade de Taubaté (UNITAU), Taubaté, SP, BrazilSuda, C.N.K., Instituto Básico de Biociências, Universidade de Taubaté (UNITAU), Taubaté, SP, Brazil, Universidade de Taubaté (UNITAU), Taubaté, SP, Brazi

Compost formation as the theme of a pedagogical project in environmental education: Vision of the school community as evaluated by content analysis [Compostagem e educação ambiental: Visão da comunidade escolar avaliada pelo método da análise de conteúdo]

Made available in DSpace on 2019-09-12T16:26:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2016One of the major environmental problems today is the large quantity of residues produced daily. In the elementary and secondary schools, Tenente Rêgo Barros (ETRB), in the city of Belém (PA), about 3 tons of dry material are produced each semester in the schools’ green spaces. To dispose of this material in an environmentally friendly manner and to educate the pupils about environmental responsibility, an interdisciplinary and transversal project has been developed. The project uses composting as a method of environmental education and meaningful learning. This work aims to analyse the interests of pedagogical advisors, teachers, school employees and the parents of ETRB students, to examine the degree to which academic staff are involved. The study used two instruments to collect the data, a questionnaire and a freehand drawing subjected to content analysis. The results indicate that the community is in favor of the project but only 20% of the teachers could see the educative utility of the compost formation. About 70% of the school community saw the production of organic fertilizer as an end in itself and as a solution for the disposal of organic residue. Most of the faculty and employees saw the formation of compost as being restricted to a scientific subject. Hence, what is needed is a discussion by the teachers on the interdisciplinary and transversal nature of the compost formation to attain the objective of environmental education. © 2016, Institute for Environmental Research in Hydrographic Basins (IPABHi). All rights reserved.do Carmo de Oliveira Alcântara, M., Universidade de Taubaté (UNITAU), Taubaté, SP, BrazilSree Vani, G., Universidade de Taubaté (UNITAU), Taubaté, SP, Brazilda Silva Almeida, A.A., Universidade de Taubaté (UNITAU), Taubaté, SP, BrazilSuda, C.N.K., Universidade de Taubaté (UNITAU), Taubaté, SP, Brazi