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Effectiveness of Gamma Rays on Virus and Viroid Diseases of Monreal Clementine and its Recovery by Shoot-Tip Grafting
Sviluppo di un metodo per l'analisi della vitalita' di protoplasti di arancio in presenza di siringomicina mediante l'uso di tecniche di citometria a flusso
Flow cytometric characterizatio and sorting of tobacco and orange protoplasts in the presence of Pseudomonas syringae phytotoxin syringomycin
Essential Oils of New Pigmented Citrus Hybrids: Citrus sinensis L. Osbeck x C clementina Hort. ex Tanaka
Micrografting of Protea cynaroides
The inability to induce rooting of in vitro-established Protea cynaroides microshoots has prevented the production of complete plantlets. A successful shoot-tip micrografting technique was developed using in vitro-germinated P. cynaroides seedlings as rootstocks and axenic microshoots established from pot plants as microscions. Thirty-day old seedlings, germinated on growth-regulator-free, half-strength Murashige and Skoog medium, were decapitated and a vertical incision made from the top end. The bottom ends of microshoots established on modified Murashige and Skoog medium were cut into a wedge (‘V’) shape, and placed into the incision. The micrografted explants were cultured in a growth chamber with the temperature adjusted to 25 ± 2°C, with a 12-h photoperiod. Best results were obtained by placing the microscions directly onto the rootstock without any pre-treatments. Dipping the explants in anti-oxidant solution or placing a layer of medium around the graft area led to the blackening of the microscion.
Abbreviations EDTA Ethylenediaminetetraacetate - BAP 6-Benzylaminopurine - GA3 Gibberellic acid - PAR Photosynthetic active radiatio
Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon ( Citrus limon
ORGANOGÊNESE IN VITRO DE Citrus EM FUNÇÃO DE CONCENTRAÇÕES DE BAP E SECCIONAMENTO DO EXPLANTE CITRUS IN VITRO ORGANOGENESIS RELATED TO BAP CONCENTRATIONS AND EXPLANT SECTION
O sucesso de técnicas biotecnológicas no melhoramento in vitro de Citrus depende diretamente do desenvolvimento de protocolos eficientes para regeneração de plantas. Objetivou-se avaliar o efeito de concentrações de 6-benzilaminopuria (BAP) na organogênese in vitro de limão-'Cravo' e laranja-'Pêra', bem como o efeito do seccionamento do explante em laranja-'Valência'. Para o limão-'Cravo', foram utilizados como explante, segmentos internodais de plântulas germinadas in vitro, cultivados em meio MT e variando-se as concentrações de BAP em 0; 2,5; 5; 7,5 e 10 mg.L-1. Nas laranjas-'Pêra' e 'Valência' os explantes foram segmentos do epicótilo de plântulas germinadas in vitro. Os explantes de laranja-'Pêra' foram cultivados em meio MT variando-se as concentrações de BAP em 0; 1; 2; 3 e 4 mg.L-1. Para a laranja-'Valência', metade dos explantes foram seccionados e cultivados em meio MT acrescido de 1,0 mg.L-1 de BAP. Todas as brotações obtidas foram alongadas no meio de cultura MT + 25 g.L-1 de sacarose + 1 mg.L-1 de ácido giberélico (GA3) e enraizadas no meio MT + 25 g.L-1 de sacarose + 0,5 g.L-1 de carvão ativado + 1 mg.L-1 de ácido naftaleno acético (ANA). O melhor resultado para o número de brotações adventícias foi obtido na concentração 2,5 mg.L-1 de BAP para limão-'Cravo', e nas concentrações 1,0 e 2,0 mg.L-1 de BAP para laranja-'Pêra'. O seccionamento dos explantes favoreceu a organogênese in vitro da laranja-'Valência', porém as brotações apresentaram menor índice de enraizamento.<br>The establishment of efficient plant regeneration protocols is essential for the success and application of in vitro breeding biotechnologies in Citrus. The objective of this work was to verify the effect of 6-benzilaminopurine (BAP) on the in vitro organogenesis of Rangpur lime (Citrus limonia (L.) Osbeck) and 'Pera' sweet orange (Citrus sinensis (L.) Osbeck), and the effect of cutting the explant on the in vitro organogenesis of 'Valencia' sweet orange (Citrus sinensis (L.) Osbeck). For Rangpur lime, internodal explants were cultured in MT medium, with 25g.L-1 sucrose and supplemented with different concentrations of BAP (0; 2.5; 5; 7.5; 10 mg.L-1). Epicotyl explants were used for 'Pera' and 'Valencia' sweet oranges. For 'Pera' sweet orange, the explants were cultured in MT medium, with 25g.L-1 sucrose and supplemented with different concentrations of BAP (0; 1; 2; 3; 4 mg.L-1). For 'Valencia' sweet orange, the epicotyl explants were cut in half and cultured on MT medium, with 25 g.L-1 of sucrose and 1 mg.L-1 of BAP. Shoot elongation was accomplished on MT + 25 g.L-1 sucrose + 1 mg.L-1 gibberelic acid (GA3) and rooted on MT + 25 g.L-1 of sucrose + 0,5 g.L-1 of activated charcoal + 1 mg.L-1 NAA. The best results for number of buds was 2.5 mg.L-1 of BAP in Rangpur lime and 1 and 2 mg.L-1 in 'Pera' sweet orange. The cut of the explant in half was favorable to the in vitro organogenesis in 'Valencia' sweet orange, but it was not for rooting