4 research outputs found
The stiffness of living tissues and its implications for tissue engineering
The past 20 years have witnessed ever- growing evidence that the mechanical
properties of biological tissues, from nanoscale to macroscale dimensions, are fundamental
for cellular behaviour and consequent tissue functionality. This knowledge, combined with
previously known biochemical cues, has greatly advanced the field of biomaterial development,
tissue engineering and regenerative medicine. It is now established that approaches to engineer
biological tissues must integrate and approximate the mechanics, both static and dynamic,
of native tissues. Nevertheless, the literature on the mechanical properties of biological tissues
differs greatly in methodology, and the available data are widely dispersed. This Review gathers
together the most important data on the stiffness of living tissues and discusses the intricacies
of tissue stiffness from a materials perspective, highlighting the main challenges associated
with engineering lifelike tissues and proposing a unified view of this as yet unreported topic.
Emerging advances that might pave the way for the next decadeâ s take on bioengineered tissue
stiffness are also presented, and differences and similarities between tissues in health and disease
are discussed, along with various techniques for characterizing tissue stiffness at various
dimensions from individual cells to organs.The authors would like to acknowledge financial support from the European Research
Council, grant agreement ERC-2012-ADG 20120216-321266 (project ComplexiTE).
C.F.G. acknowledges scholarship grant no. PD/BD/135253/2017 from Fundação para a
Ciência e Tecnologia (FCT). The authors also thank the peer-reviewers for the
constructive comments and suggestions that helped to shape this manuscript