"Hero Imagery" - Are there performance advantages associated with imagining yourself as your favourite athlete?

Objectives: This study examined whether there are performance advantages associated with a single bout of imagery when imagining yourself ‘as your favourite athlete’, or imagining yourself performing a strength-based task. Design: A blind 2 (Imagery ability: high, low) x 3 (imagery condition: self, “hero”, control) mixed factorial design was used. Methods: Participants (n = 17 male; Mage = 19.7 ± 2.7) completed the Sport Imagery Ability Questionnaire then viewed a standardised video demonstrating the grip strength (GS) task. Three baseline trials separated by one minute were then executed. Three imagery scripts (control, self, hero) were then presented to participants via an MP3 player in a counterbalanced order (an interval of 1-minute was provided between each condition). The conclusion of each imagery script prompted participants to perform the GS task. Performance in each condition was conceptualised as delta change scores (Imagery condition – baseline average). Results: No main effects were present but there was a group x condition interaction (F(2,28) = 4.27, p = .02. ƞ_p^2= .23. The interaction suggests that for individuals with high imagery ability, simply “doing the imagery that they already do” is preferable compared to a scripted self- or hero-imagery condition. For individuals with a low imagery ability, a simple script whether that is self- or hero- based may enhance strength performance, compared to “what they already do”. Conclusion: Imagery ability may influence the effectiveness of a brief imagery intervention. Further examination of processes and outcomes associated with “hero-imagery” is recommended

Organic Matter Preservation and Incipient Mineralization of Microtubules in 120 Ma Basaltic Glass

Hollow tubular structures in subaqueously-emplaced basaltic glass may represent trace fossils caused by microbially-mediated glass dissolution. Mineralized structures of similar morphology and spatial distribution in ancient, metamorphosed basaltic rocks have widely been interpreted as ichnofossils, possibly dating to similar to 3.5 Ga or greater. Doubts have been raised, however, regarding the biogenicity of the original hollow tubules and granules in basaltic glass. In particular, although elevated levels of biologically-important elements such as C, S, N, and P as well as organic compounds have been detected in association with these structures, a direct detection of unambiguously biogenic organic molecules has not been accomplished. In this study, we describe the direct detection of proteins associated with tubular textures in basaltic glass using synchrotron X-ray spectromicroscopy. Protein-rich organic matter is shown to be associated with the margins of hollow and partly-mineralized tubules. Furthermore, a variety of tubule-infilling secondary minerals, including Ti-rich oxide phases, were observed filling and preserving the microtextures, demonstrating a mechanism whereby cellular materials may be preserved through geologic time

Association of a functional microsatellite within intron 1 of the BMP5 gene with susceptibility to osteoarthritis

<p>Abstract</p> <p>Background</p> <p>In a previous study carried out by our group, the genotyping of 36 microsatellite markers from within a narrow interval of chromosome 6p12.3-q13 generated evidence for linkage and for association to female hip osteoarthritis (OA), with the most compelling association found for a marker within intron 1 of the bone morphogenetic protein 5 gene (<it>BMP5</it>). In this study, we aimed to further categorize the association of variants within intron 1 of <it>BMP5 </it>with OA through an expanded genetic association study of the intron and subsequent functional analysis of associated polymorphisms.</p> <p>Methods</p> <p>We genotyped 18 common polymorphisms including 8 microsatellites and 9 single nucleotide polymorphisms (SNPs) and 1 insertion/deletion (INDEL) from within highly conserved regions between human and mouse within intron 1 of <it>BMP5</it>. These markers were then tested for association to OA by a two-stage approach in which the polymorphisms were initially genotyped in a case-control cohort comprising 361 individuals with associated polymorphisms (<it>P </it>≤ 0.05) then genotyped in a second case-control cohort comprising 1185 individuals.</p> <p>Results</p> <p>Two <it>BMP5 </it>intron 1 polymorphisms demonstrated association in the combined case-control cohort of 1546 individuals (765 cases and 781 controls): microsatellite D6S1276 (<it>P </it>= 0.018) and SNP rs921126 (<it>P </it>= 0.013). Functional analyses in osteoblastic, chondrocytic, and adipocytic cell lines indicated that allelic variants of D6S1276 have significant effects on the transcriptional activity of the <it>BMP5 </it>promoter <it>in vitro</it>.</p> <p>Conclusion</p> <p>Variability in gene expression of <it>BMP5 </it>may be an important contributor to OA genetic susceptibility.</p

Metabolomics Reveals Target and Off-Target Toxicities of a Model Organophosphate Pesticide to Roach (Rutilus rutilus): Implications for Biomonitoring

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Demonstrating the reliability of in vivo metabolomics based chemical grouping:towards best practice

While grouping/read-across is widely used to fill data gaps, chemical registration dossiers are often rejected due to weak category justifications based on structural similarity only. Metabolomics provides a route to robust chemical categories via evidence of shared molecular effects across source and target substances. To gain international acceptance, this approach must demonstrate high reliability, and best-practice guidance is required. The MetAbolomics ring Trial for CHemical groupING (MATCHING), comprising six industrial, government and academic ring-trial partners, evaluated inter-laboratory reproducibility and worked towards best-practice. An independent team selected eight substances (WY-14643, 4-chloro-3-nitroaniline, 17α-methyl-testosterone, trenbolone, aniline, dichlorprop-p, 2-chloroaniline, fenofibrate); ring-trial partners were blinded to their identities and modes-of-action. Plasma samples were derived from 28-day rat tests (two doses per substance), aliquoted, and distributed to partners. Each partner applied their preferred liquid chromatography–mass spectrometry (LC–MS) metabolomics workflows to acquire, process, quality assess, statistically analyze and report their grouping results to the European Chemicals Agency, to ensure the blinding conditions of the ring trial. Five of six partners, whose metabolomics datasets passed quality control, correctly identified the grouping of eight test substances into three categories, for both male and female rats. Strikingly, this was achieved even though a range of metabolomics approaches were used. Through assessing intrastudy quality-control samples, the sixth partner observed high technical variation and was unable to group the substances. By comparing workflows, we conclude that some heterogeneity in metabolomics methods is not detrimental to consistent grouping, and that assessing data quality prior to grouping is essential. We recommend development of international guidance for quality-control acceptance criteria. This study demonstrates the reliability of metabolomics for chemical grouping and works towards best-practice

Clinician and Parent Perspectives on Parent and Family Contextual Factors that Impact Community Mental Health Services for Children with Behavior Problems

The present study employed qualitative methods to examine multiple stakeholder perspectives regarding the role of parent and family contextual factors on community child mental health treatment for children with behavior problems. Findings suggest agreement between clinicians and parents on the number, types and importance of parent and family factors in children’s mental health services; however, stakeholders differed in reports of which factors were most salient. Specifically, clinicians endorsed most factors as being equally salient, while parents described a few salient factors, with parental stress and inadequate social support being the most frequently discussed. These qualitative data further elucidate the context of community services and have implications for evidence-based practice implementation and improving community care

Direct peptide bioconjugation/PEGylation at tyrosine with linear and branched polymeric diazonium salts

Direct polymer conjugation at peptide tyrosine residues is described. In this study Tyr residues of both leucine enkephalin and salmon calcitonin (sCT) were targeted using appropriate diazonium salt-terminated linear monomethoxy poly(ethylene glycol)s (mPEGs) and poly(mPEG) methacrylate prepared by atom transfer radical polymerization. Judicious choice of the reaction conditions-pH, stoichiometry, and chemical structure of diazonium salt-led to a high degree of site-specificity in the conjugation reaction, even in the presence of competitive peptide amino acid targets such as histidine, lysines, and N-terminal amine. In vitro studies showed that conjugation of mPEG 2000 to sCT did not affect the peptide's ability to increase intracellular cAMP induced in T47D human breast cancer cells bearing sCT receptors. Preliminary in vivo investigation showed preserved ability to reduce [Ca 2+] plasma levels by mPEG 2000-sCT conjugate in rat animal models. © 2012 American Chemical Society

Assessment of learning outcomes workshop: How do you know that your assessment tasks are effective?

Background Higher Education in Australia is in a phase of rapid change due to regulatory changes (TEQSA) and a shift towards a standards based framework. Over the past five years, the Chemistry community in Australia has developed the Chemistry Threshold Learning Outcomes (CTLOs) which articulate the outcomes that every student graduating from an Australian university with a major in Chemistry will have attained. In keeping with this development, the Royal Australian Chemical Institute (RACI) now bases its accreditation for Chemistry degrees on the CTLOs. Therefore, it is now vital to the Chemistry community to ensure that the assessment items we use allow students to demonstrate attainment of all CTLOs during their chemistry degree. Our OLT funded project (Assessing the assessments: Evidencing and benchmarking student learning outcomes in Chemistry (OLT ID14-3562)) has developed a diagnostic framework that will help you to determine whether your assessment items actually deliver reliable measures of student performance and provide evidence of achievement of the CTLOs. An additional outcome of the project will be a database of standards-based assessment items to be shared with the Chemistry community. Workshop Format We invite you to attend this half-day workshop where project team members will guide you through evaluating your assessment items for their ‘fitness for purpose’ in providing evidence of achievement of the CTLOs. Ideally you will bring along one of your 2nd or 3rd year Chemistry assessment items (in electronic format) so that the workshop team can guide you through an online submission and evaluation to determine: 1. Which CTLOs are explicitly demonstrated by students through successful completion of the item? 2. Is your task suited to a developing or graduate level understanding? 3. To what extent can your task be said to help confirm student attainment of the CTLOs? A critical aspect of this process is consideration of marking schemes and student work for the assessment items. We strongly encourage you to bring one or two pieces of marked pass level student work (de-identified) that can be used to evidence successful attainment of a particular CTLO. A central part of the workshop will be a ‘calibration’ exercise, which allows developing a mutual understanding of what constitutes demonstrating attainment of a particular CTLO. While the content of this workshop is Chemistry specific, the process is not. Therefore we are confident that all ACSME attendees will find this to be a valuable experience. All are invited. Attendance is free, however, registration is required for catering purposes. Proceedings of the Australian Conference on Science and Mathematics Education, The University of Queensland, Sept 28th to 30th, 2016, page X, ISBN Number 978-0-9871834-4-6

Family treatment of child anxiety: outcomes, limitations and future directions

Anxiety of childhood is a common and serious condition. The past decade has seen an increase in treatment-focussed research, with recent trials tending to give greater attention to parents in the treatment process. This review examines the efficacy of family-based cognitive behaviour therapy and attempts to delineate some of the factors that might have an impact on its efficacy. The choice and timing of outcome measure, age and gender of the child, level of parental anxiety, severity and type of child anxiety and treatment format and content are scrutinised. The main conclusions are necessarily tentative, but it seems likely that Family Cognitive Behaviour Therapy (FCBT) is superior to no treatment, and, for some outcome measures, also superior to Child Cognitive Behaviour Therapy (CCBT). Where FCBT is successful, the results are consistently maintained at follow-up. It appears that where a parent is anxious, and this is not addressed, outcomes are less good. However, for children of anxious parents, FCBT is probably more effective than CCBT. What is most clear is that large, well-designed studies, examining these factors alone and in combination, are now needed