Histone hyperacetylation disrupts core gene regulatory architecture in rhabdomyosarcoma

Core regulatory transcription factors (CR TFs) orchestrate the placement of super-enhancers (SEs) to activate transcription of cell-identity specifying gene networks, and are critical in promoting cancer. Here, we define the core regulatory circuitry of rhabdomyosarcoma and identify critical CR TF dependencies. These CR TFs build SEs that have the highest levels of histone acetylation, yet paradoxically the same SEs also harbor the greatest amounts of histone deacetylases. We find that hyperacetylation selectively halts CR TF transcription. To investigate the architectural determinants of this phenotype, we used absolute quantification of architecture (AQuA) HiChIP, which revealed erosion of native SE contacts, and aberrant spreading of contacts that involved histone acetylation. Hyperacetylation removes RNA polymerase II (RNA Pol II) from core regulatory genetic elements, and eliminates RNA Pol II but not BRD4 phase condensates. This study identifies an SE-specific requirement for balancing histone modification states to maintain SE architecture and CR TF transcription

Context Analysis of User Decision based on social learning

We analyzed the context mechanism of usersâ?? decision behaviors in social tagging, and revealed the nature of social learning among users in social contexts. To improve the results of social tagging, we can select corresponding contexts according to usersâ?? given knowledge backgrounds and preferences. On the other hand, we can also recognize usersâ?? knowledge backgrounds and preferences according to their annotation results in relevant contexts

A novel approach to the growth analysis of hamster secondary palate by histone 3 m RNA in situ hybridiration

A study was undertaken to determine the cell proliferation kinetics during the development of hamster vertical palatal shelf ad initium. Harnster embryo heads, obtained at different times between days 10 and 12 of gestation (which is the period of vertical shelf development) were processed and sectioned to localize histone 3 mRNA, a cell cycle specific gene, by in situ hybridization. Sense and antisense 35~-labelledh istone 3 riboprobes were used as hybridization probes. Percent labelled cells were determined. The results showed that a high rate of random proliferation of both epithelial and mesenchymal cells was a major component of early vertical palatal growth. Subsequently, during the latter half of vertical shelf development, the proliferation rates of the epithelial and mesenchymal cells were different in a region specific manner. It was suggested that the spatio-temporal changes in the distribution of cycling mesenchymal and epithelial cells during vertical palate development may indicate their heterogeneity for subsequent segregation into appropnate phenotypes

A dual-mode fluorescence "turn-on" biosensor based on an aggregation-induced emission luminogen

10.1039/c3tb21576hJournal of Materials Chemistry B2121717-1723JMCB

A unimolecular theranostic system with H2O2-specific response and AIE-activity for doxorubicin releasing and real-time tracking in living cells

10.1039/c8ra01185kRSC Advances82010975-1097