10,250 research outputs found
Efeito da água residuária do café em plantas de milho.
O processamento do café por via úmida gera água residuária (ARC), contendo material orgânico, com potencial de poluir o meio aquático. Este trabalho teve como objetivo estudar o efeito da aplicação da ARC sobre o crescimento e teores de minerais em plantas de milho, na fase vegetativa. Foram estudados oito tratamentos: 0, 50, 100, 150, 200, 250, 300 e 350 mL de ARC por planta, aplicada sobre as folhas, em 3 ocasiões. A aplicação da ARC não provocou injúrias visíveis nas plantas de milho e nem alterou o peso da matéria seca das plantas. O teor de K aumentou e o teor de Mg diminuiu linearmente com o aumento das doses de ARC aplicadas, enquanto os teores de Ca e Na nas folhas de milho não foram afetados
Neuromorphometric characterization with shape functionals
This work presents a procedure to extract morphological information from
neuronal cells based on the variation of shape functionals as the cell geometry
undergoes a dilation through a wide interval of spatial scales. The targeted
shapes are alpha and beta cat retinal ganglion cells, which are characterized
by different ranges of dendritic field diameter. Image functionals are expected
to act as descriptors of the shape, gathering relevant geometric and
topological features of the complex cell form. We present a comparative study
of classification performance of additive shape descriptors, namely, Minkowski
functionals, and the nonadditive multiscale fractal. We found that the proposed
measures perform efficiently the task of identifying the two main classes alpha
and beta based solely on scale invariant information, while also providing
intraclass morphological assessment
Hyaluronan density influences adhesion, morphology and migration of cancer cells
Hyaluronan (HA) is a linear non-sulfated glycosaminoglycan present in the extracellular matrix and known to modulate cell-cell and cell-ECM interactions. In cancer, the synthesis, degradation and signaling of HA is altered. For instance, its main receptor, CD44, is overexpressed in several types of cancer and has been correlated with disease progression through cancer cell proliferation, migration and chemoresistance. Herein, we investigated the behavior of breast cancer cells with different CD44 expression and invasion profile on HA density gradients. These gradients were achieved by deposition of colloidal gold (Au) on amino-functionalized surfaces at different ionic strengths and following binding of end-on thiol modified HA on the Au. At low HA density, small number of adherent round cells were found for all studied cell lines. Cells adherent to the areas with high HA density presented a spindle-like morphology. The differences were more pronounced for cells overexpressing CD44. These cells also form long filopodia when adhered on areas with middle and high HA density. Of note, colocalization of CD44 and actin was observed at the filopodias edges. Cell motility was also affected by the gradient – at low densities cells presented higher motility, which decreased with the increase of HA density. Besides this common trend, we observed differences among the studied cells. CD44 cells had shorter persistent length displacement than CD44 and CD44cells. Upon CD44 blockage, all types of cells (CD44++, CD44+, and CD44-) behave similarly . These results suggest that cells recognize HA gradients through CD44 receptors and that the HA density can be used to sort cells with different expression of this receptor.info:eu-repo/semantics/publishedVersio
Comportamento de mudas enxertadas e crescimento in vitro do Fusarium oxysporum f. sp. passiflorae em diferentes pH do meio.
O maracujá amarelo é plantado em quase todas as regiões brasileiras. O Nordeste produz 76% da produção, com destaque para a Bahia, com 50% da produção do país. Apesar da posição de destaque, a vida útil do maracujazeiro vem sendo reduzida, principalmente, devido aos danos causados por doenças do sistema radicular com destaque para a fusariose (Fusarium oxysporum f. sp. Passiflorae ? Fop)
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